Transcript: Human XM_005266061.5

PREDICTED: Homo sapiens NMDA receptor synaptonuclear signaling and neuronal migration factor (NSMF), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NSMF (26012)
Length:
2884
CDS:
218..1714

Additional Resources:

NCBI RefSeq record:
XM_005266061.5
NBCI Gene record:
NSMF (26012)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005266061.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000165267 GTGTCTGACGACATCCCTATT pLKO.1 839 CDS 100% 10.800 15.120 N NSMF n/a
2 TRCN0000165544 GACGTACTTTGACTTCCGGTT pLKO.1 1633 CDS 100% 2.160 3.024 N NSMF n/a
3 TRCN0000165939 GCAGATGATCGAGACGTACTT pLKO.1 1621 CDS 100% 4.950 3.960 N NSMF n/a
4 TRCN0000165391 CCAGACAGCAACCACAACTAT pLKO.1 895 CDS 100% 5.625 3.938 N NSMF n/a
5 TRCN0000165511 GAGAATGATTCCGCGTCTGTA pLKO.1 962 CDS 100% 4.950 3.465 N NSMF n/a
6 TRCN0000165955 GCTGCTGTTGAGTTCAGCTTT pLKO.1 2495 3UTR 100% 4.950 3.465 N NSMF n/a
7 TRCN0000164926 GAAGCTGAACGTCTACCACAA pLKO.1 1300 CDS 100% 4.050 2.835 N NSMF n/a
8 TRCN0000161947 GCTTTCACTTACACTCCTCTT pLKO.1 2316 3UTR 100% 4.050 2.835 N NSMF n/a
9 TRCN0000165475 GAAGCAGATGATCGAGACGTA pLKO.1 1618 CDS 100% 2.640 1.848 N NSMF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005266061.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02903 pDONR223 100% 94.3% 94.3% None 825_826ins90 n/a
2 ccsbBroad304_02903 pLX_304 0% 94.3% 94.3% V5 825_826ins90 n/a
3 TRCN0000478064 GAGTCTTCTGTGTTCATACAGTCC pLX_317 2.4% 94.3% 94.3% V5 825_826ins90 n/a
4 ccsbBroadEn_02902 pDONR223 100% 89.9% 89.9% None 705_773del;825_826ins90 n/a
5 ccsbBroad304_02902 pLX_304 0% 89.9% 89.9% V5 705_773del;825_826ins90 n/a
6 TRCN0000465661 CGCGGCTGACCCGATGTCTGCTGA pLX_317 17% 89.9% 89.9% V5 705_773del;825_826ins90 n/a
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