Construct: ORF TRCN0000465661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013700.1_s317c1
- Derived from:
- ccsbBroadEn_02902
- DNA Barcode:
- CGCGGCTGACCCGATGTCTGCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSMF (26012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130971.1 | 100% | 100% | |
| 2 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130970.1 | 99.6% | 99.6% | 704_709delCCATCT |
| 3 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_015537.4 | 95.6% | 95.6% | 705_773del |
| 4 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001130969.1 | 95.2% | 95.2% | 705_779del |
| 5 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_005266062.5 | 94% | 94% | 756_757ins90 |
| 6 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_011518496.3 | 93.6% | 93.6% | 704_709delCCATCT;762_763ins90 |
| 7 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_005266061.5 | 89.9% | 89.9% | 705_773del;825_826ins90 |
| 8 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | NM_001178064.1 | 89.6% | 89.6% | 705_779del;831_832ins90 |
| 9 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_017014597.2 | 62% | 47.1% | (many diffs) |
| 10 | human | 26012 | NSMF | NMDA receptor synaptonuclea... | XM_011518497.2 | 52% | 52% | 0_1ins726 |
| 11 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498200.1 | 88.9% | 93.6% | (many diffs) |
| 12 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_020276.3 | 88.5% | 93.3% | (many diffs) |
| 13 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001039387.1 | 85.1% | 89.6% | (many diffs) |
| 14 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001039386.1 | 84.7% | 89.2% | (many diffs) |
| 15 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001177654.1 | 84.6% | 89% | (many diffs) |
| 16 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498203.3 | 83.2% | 87.7% | (many diffs) |
| 17 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498202.3 | 82.9% | 87.4% | (many diffs) |
| 18 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319143.1 | 79.8% | 84% | (many diffs) |
| 19 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | NM_001177655.1 | 79.6% | 83.9% | (many diffs) |
| 20 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319142.1 | 79.5% | 83.7% | (many diffs) |
| 21 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_006498201.1 | 79.3% | 83.6% | (many diffs) |
| 22 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319141.1 | 76.7% | 80.7% | (many diffs) |
| 23 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319140.1 | 76.4% | 80.5% | (many diffs) |
| 24 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319144.1 | 58.5% | 56.4% | (many diffs) |
| 25 | mouse | 56876 | Nsmf | NMDA receptor synaptonuclea... | XM_017319145.1 | 55.3% | 58.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1584
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgccgcc gcctccagga ggagggcgct gaggagcgag gccatgtcct 121 cggtggcggc caaagtgcga gcagcccgag cgtttggaga gtacctgtcc cagagtcacc 181 ctgagaaccg caacggcgca gatcacctgc tggctgatgc ctactctggc cacgacgggt 241 cccccgagat gcagccggcc ccccagaaca agcgccgcct gtccctcgtc tccaacggct 301 gctacgaggg cagcctctca gaggagccca gcattaggaa gcccgcaggc gagggccctc 361 agcctcgagt gtacaccatc tctggggagc ctgccctgct gcccagccct gaggcggagg 421 ccattgagct ggcggtggtg aaggggcggc ggcagcggca ccctcaccat cacagccagc 481 ccctgcgcgc cagccctggt ggcagccggg aggacgtcag caggccctgc cagagctggg 541 cgggcagccg ccagggctcc aaggagtgcc ccggatgtgc ccagctggct cctggcccca 601 cccctcgggc ctttgggctg gaccagccac ctctgcctga gacctccggt cgccgcaaga 661 agctggagag gatgtacagc gttgaccgtg tgtctgacga catccctatt cgtacctggt 721 tccccaagga aaatcttttc agcttccaga cagcaaccac aactatgcaa gcgaacttcc 781 gcaaacacct gcgcatggtc ggcagccgga gggtgaaggc ccagacgttc gctgagcggc 841 gcgagcggag cttcagccgg tcctggagcg accccacccc catgaaagcc gacacttccc 901 acgactcccg agacagcagt gacctgcaga gctcccactg cacgctggac gaggccttcg 961 aggacctgga ctgggacact gagaagggcc tggaggctgt ggcctgcgac accgaaggct 1021 tcgtgccacc aaaggtcatg ctcatttcct ccaaggtgcc caaggctgag tacatcccca 1081 ctatcatccg ccgggatgac ccctccatca tccccatcct ctacgaccat gagcacgcaa 1141 ccttcgagga catccttgag gagatagaga ggaagctgaa cgtctaccac aagggagcca 1201 agatctggaa aatgctgatt ttctgccagg gaggtcctgg acacctctat ctcctcaaga 1261 acaaggtggc cacctttgcc aaagtggaga aggaagagga catgattcac ttctggaagc 1321 ggctgagccg ccTGATGAGC AAAGTGAACC CAGAGCCGAA CGTCATCCAC ATCATGGGCT 1381 GCTACATTCT GGGGAACCCC AATGGAGAGA AGCTGTTCCA GAACCTCAGG ACCCTCATGA 1441 CTCCTTATAG GGTCACCTTC GAGTCACCCC TGGAGCTCTC AGCCCAAGGG AAGCAGATGA 1501 TCGAGACGTA CTTTGACTTC CGGTTGTATC GCCTGTGGAA GAGCCGCCAG CACTCGAAGC 1561 TGCTGGACTT TGACGACGTC CTGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GCGGCTGACC 1741 CGATGTCTGC TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att