Transcript: Human XM_011518063.2

PREDICTED: Homo sapiens ADAMTS like 1 (ADAMTSL1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ADAMTSL1 (92949)
Length:
7981
CDS:
93..5579

Additional Resources:

NCBI RefSeq record:
XM_011518063.2
NBCI Gene record:
ADAMTSL1 (92949)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011518063.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000118534 CCGCAACCAAATCGGATGATA pLKO.1 880 CDS 100% 5.625 7.875 N ADAMTSL1 n/a
2 TRCN0000118533 CGCGTTGCTATACAGAATCTT pLKO.1 715 CDS 100% 5.625 7.875 N ADAMTSL1 n/a
3 TRCN0000118532 GCGTTGCTATACAGAATCTTT pLKO.1 716 CDS 100% 5.625 7.875 N ADAMTSL1 n/a
4 TRCN0000140380 GCTGGACAGATCCTTCAAGTT pLKO.1 4638 CDS 100% 4.950 3.960 N ADAMTSL1 n/a
5 TRCN0000118536 GCAGATTTCATTGTCAAGATT pLKO.1 1107 CDS 100% 5.625 3.938 N ADAMTSL1 n/a
6 TRCN0000140700 GCTCATGCAGAAGGCATCTTT pLKO.1 4721 CDS 100% 5.625 3.938 N ADAMTSL1 n/a
7 TRCN0000118535 CCGATACAGAACATGCAGTAA pLKO.1 503 CDS 100% 4.950 3.465 N ADAMTSL1 n/a
8 TRCN0000143637 GAAGTCACTTGGTTCAGGAAT pLKO.1 4239 CDS 100% 4.950 3.465 N ADAMTSL1 n/a
9 TRCN0000143687 GCAGAAGGCATCTTTAGTGAT pLKO.1 4727 CDS 100% 4.950 3.465 N ADAMTSL1 n/a
10 TRCN0000080459 GCCAGATGATTCCTTACAGAT pLKO.1 3980 CDS 100% 4.950 3.465 N Adamtsl1 n/a
11 TRCN0000140786 GCCAGAAGGTTTGTAGCCTAT pLKO.1 5929 3UTR 100% 4.050 2.835 N ADAMTSL1 n/a
12 TRCN0000143299 GAAGGCATCTTTAGTGATCCA pLKO.1 4730 CDS 100% 2.640 1.848 N ADAMTSL1 n/a
13 TRCN0000139720 CCCAGAACTCACTCAGTTCTT pLKO.1 6107 3UTR 100% 0.495 0.347 N ADAMTSL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011518063.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12984 pDONR223 100% 24.8% 24.5% None (many diffs) n/a
2 ccsbBroad304_12984 pLX_304 0% 24.8% 24.5% V5 (many diffs) n/a
3 TRCN0000469304 CTCCTGCAATCATGGACCCCAGTC pLX_317 27.8% 24.8% 24.5% V5 (many diffs) n/a
4 ccsbBroadEn_16067 pDONR223 0% 23.5% 22.8% None (many diffs) n/a
5 ccsbBroad304_16067 pLX_304 0% 23.5% 22.8% V5 (many diffs) n/a
6 TRCN0000465309 ATGAAACTCTTAGAACAAGTGTAC pLX_317 24.1% 23.5% 22.8% V5 (many diffs) n/a
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