Construct: ORF TRCN0000465309
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014801.1_s317c1
- Derived from:
- ccsbBroadEn_16067
- DNA Barcode:
- ATGAAACTCTTAGAACAAGTGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAMTSL1 (92949)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465309
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | NM_052866.5 | 83% | 81.3% | (many diffs) |
| 2 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_017015314.1 | 28.7% | 27.9% | (many diffs) |
| 3 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | NM_001040272.6 | 24.7% | 24.4% | (many diffs) |
| 4 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_017015312.2 | 24% | 23.1% | (many diffs) |
| 5 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_011518064.3 | 23.8% | 22.9% | (many diffs) |
| 6 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_017015311.1 | 23.7% | 23% | (many diffs) |
| 7 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_017015310.1 | 23.5% | 22.9% | (many diffs) |
| 8 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_011518063.2 | 23.5% | 22.8% | (many diffs) |
| 9 | human | 92949 | ADAMTSL1 | ADAMTS like 1 | XM_017015313.1 | 16.2% | 15.9% | (many diffs) |
| 10 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | NM_029967.3 | 22.3% | 23.4% | (many diffs) |
| 11 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | XM_006538378.3 | 22% | 23.1% | (many diffs) |
| 12 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | XM_006538377.1 | 19.8% | 20.3% | (many diffs) |
| 13 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | XM_006538376.1 | 19.4% | 20.1% | (many diffs) |
| 14 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | XM_006538375.1 | 19.2% | 20% | (many diffs) |
| 15 | mouse | 77739 | Adamtsl1 | ADAMTS-like 1 | XM_006538374.1 | 19.2% | 19.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga atgctgccgt cgggcaactc ctggcacact gctcctcttt ctggctttcc 121 tgctcctgag ttccaggacc gcacgctccg aggaggaccg ggacggccta tgggatgcct 181 ggggcccatg gagtgaatgc tcacgcacct gcgggggagg ggcctcctac tctctgaggc 241 gctgcctgag cagcaagagc tgtgaaggaa gaaatatccg atacagaaca tgcagtaatg 301 tggactgccc accagaagca ggtgatttcc gagctcagca atgctcagct cataatgatg 361 tcaagcacca tggccagttt tatgaatggc ttcctgtgtc taatgaccct gacaacccat 421 gttcactcaa gtgccaagcc aaaggaacaa ccctggttgt tgaactagca cctaaggtct 481 tagatggtac gcgttgctat acagaatctt tggatatgtg catcagtggt ttatgccaaa 541 ttgttggctg cgatcaccag ctgggaagca ccgtcaagga agataactgt ggggtctgca 601 acggagatgg gtccacctgc cggctggtcc gagggcagta taaatcccag ctctccgcaa 661 ccaaatcgga tgatactgtg gttgcaattc cctatggaag tagacatatt cgccttgtct 721 taaaaggtcc tgatcactta tatctggaaa ccaaaaccct ccaggggact aaaggtgaaa 781 acagtctcag ctccacagga actttccttg tggacaattc tagtgtggac ttccagaaat 841 ttccagacaa agagatactg agaatggctg gaccactcac agcagatttc attgtcaaga 901 ttcgtaactc gggctccgct gacagtacag tccagttcat cttctatcaa cccatcatcc 961 accgatggag ggagacggat ttctttcctt gctcagcaac ctgtggagga ggttatcagc 1021 tgacatcggc tgagtgctac gatctgagga gcaaccgtgt ggttgctgac CAATACTGTC 1081 ACTATTACCC AGAGAACATC AAACCCAAAC CCAAGCTTCA GGAGTGCAAC TTGGATCCTT 1141 GTCCAGCCAG GTGGGAGGCC ACCCCATGGA CCGCGTGCTC CTCCTCGTGT GGGGGGGGCA 1201 TCCAGAGCCG GGCAGTTTCC TGTGTGGAGG AGGACATCCA GGGGCATGTC ACTTCAGTGG 1261 AAGAGTGGAA ATGCATGTAC ACCCCTAAGA TGCCCATCGC GCAGCCCTGC AACATTTTTG 1321 ACTGCCCTAA ATGGCTGGCA CAGGAGTGGT CTCCGGTAAC TGTGCCTTCT TTCTTTGTTC 1381 ATTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GAAACTCTTA GAACAAGTGT ACACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt