Transcript: Human XM_024447314.1

PREDICTED: Homo sapiens family with sequence similarity 86 member B1 (FAM86B1), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM86B1 (85002)
Length:
2989
CDS:
584..1432

Additional Resources:

NCBI RefSeq record:
XM_024447314.1
NBCI Gene record:
FAM86B1 (85002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024447314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000230959 ATGGAGAGGAGGCTAGGATAA pLKO_005 259 5UTR 100% 10.800 7.560 N FAM86B2 n/a
2 TRCN0000172312 CGAGGGAATGTCCTTCTCAAT pLKO.1 950 CDS 100% 4.950 2.475 Y EEF2KMT n/a
3 TRCN0000128827 GCTTTCTCTCAGAACTCATCA pLKO.1 593 CDS 100% 4.950 2.475 Y FAM86B1 n/a
4 TRCN0000129161 GTGCTTTCTCTCAGAACTCAT pLKO.1 591 CDS 100% 4.950 2.475 Y FAM86B1 n/a
5 TRCN0000130496 GACCAGAAACTGTTTCCCTAT pLKO.1 1431 CDS 100% 4.050 2.025 Y FAM86B1 n/a
6 TRCN0000168609 GATGTTGTCATTGCAGCAGAT pLKO.1 1082 CDS 100% 4.050 2.025 Y EEF2KMT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024447314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488399 TACCTGCGCGCCTAACAATCATGA pLX_317 37.3% 54.4% 40.7% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_09791 pDONR223 100% 54.3% 40.5% None (many diffs) n/a
3 ccsbBroad304_09791 pLX_304 0% 54.3% 40.5% V5 (many diffs) n/a
4 TRCN0000466174 GGAGAGATGACCTTCCATCATCTG pLX_317 21.3% 54.3% 40.5% V5 (many diffs) n/a
5 TRCN0000488297 ACTAGACGAAAAATGAGGCATGGA pLX_317 30.4% 54.3% 40.5% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000491961 CCGTTCATGATGGCGTAAGTACGC pLX_317 33.3% 47.3% 39.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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