Construct: ORF TRCN0000465557

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000907.1_s317c1
Derived from:
ccsbBroadEn_12945
DNA Barcode:
AGCCCGCTCACATCTTTTAGGTCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ANGEL2 (90806)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465557
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 90806 ANGEL2 angel homolog 2 NM_001300757.1 76.2% 76.2% 859_1125del
2 human 90806 ANGEL2 angel homolog 2 NM_001300758.1 76.2% 76.2% 859_1125del
3 human 90806 ANGEL2 angel homolog 2 NM_001300753.1 68.4% 68.4% 1_129del;988_1254del
4 human 90806 ANGEL2 angel homolog 2 NM_001300755.1 68.4% 68.4% 1_129del;988_1254del
5 human 90806 ANGEL2 angel homolog 2 XM_017002775.1 68.4% 68.4% 1_129del;988_1254del
6 human 90806 ANGEL2 angel homolog 2 XM_017002776.1 68.4% 68.4% 1_129del;988_1254del
7 human 90806 ANGEL2 angel homolog 2 XM_017002777.1 68.4% 68.4% 1_129del;988_1254del
8 human 90806 ANGEL2 angel homolog 2 XM_017002778.1 68.4% 68.4% 1_129del;988_1254del
9 human 90806 ANGEL2 angel homolog 2 XM_005273347.3 61.5% 61.5% 1_270del;1129_1395del
10 human 90806 ANGEL2 angel homolog 2 XM_005273344.1 54.7% 54.7% 1_441del;1300_1566del
11 human 90806 ANGEL2 angel homolog 2 XM_005273345.1 54.7% 54.7% 1_441del;1300_1566del
12 human 90806 ANGEL2 angel homolog 2 XM_005273346.2 54.7% 54.7% 1_441del;1300_1566del
13 human 90806 ANGEL2 angel homolog 2 XM_017002774.1 54.7% 54.7% 1_441del;1300_1566del
14 human 90806 ANGEL2 angel homolog 2 NM_144567.5 52.5% 52.5% 1_507del;1366_1632del
15 human 90806 ANGEL2 angel homolog 2 XR_001737527.1 17.2% 1_521del;1146_1147ins127;1253_4102del
16 human 90806 ANGEL2 angel homolog 2 XR_247045.3 16.8% 1_631del;1256_1257ins127;1363_4212del
17 human 90806 ANGEL2 angel homolog 2 XR_001737528.1 16.6% 1_399del;1024_1025ins127;1131_4259del
18 human 90806 ANGEL2 angel homolog 2 NR_125333.1 16.6% 1_385del;1010_1011ins127;1117_4264del
19 human 90806 ANGEL2 angel homolog 2 XR_001737530.1 15.7% 1_660del;1285_1286ins127;1392_4520del
20 human 90806 ANGEL2 angel homolog 2 XR_001737529.2 15.4% 1_732del;1357_1358ins127;1464_4592del
21 human 90806 ANGEL2 angel homolog 2 XR_001737532.1 14.8% 1_951del;1576_1577ins127;1683_4811del
22 human 90806 ANGEL2 angel homolog 2 XR_001737531.1 14.8% 1_952del;1577_1578ins127;1684_4812del
23 mouse 52477 Angel2 angel homolog 2 XM_006497186.2 58.6% 61.2% (many diffs)
24 mouse 52477 Angel2 angel homolog 2 XM_017321690.1 47.8% 48.5% (many diffs)
25 mouse 52477 Angel2 angel homolog 2 NM_001199020.1 46.9% 49% (many diffs)
26 mouse 52477 Angel2 angel homolog 2 XM_006497183.3 46.9% 49% (many diffs)
27 mouse 52477 Angel2 angel homolog 2 XM_006497184.3 46.9% 49% (many diffs)
28 mouse 52477 Angel2 angel homolog 2 XM_006497185.1 46.9% 49% (many diffs)
29 mouse 52477 Angel2 angel homolog 2 NM_021421.4 45% 47% (many diffs)
30 mouse 52477 Angel2 angel homolog 2 NR_037579.1 25.3% (many diffs)
31 mouse 52477 Angel2 angel homolog 2 NR_037578.1 23% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
924
ORF length:
858
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ctataatata ctttcacaag atttactgga agataactct cacctttata
121gacattgccg gcggccagta ttacactgga gttttaggtt tcccaatatt ctgaaagaaa
181ttaaacattt tgatgcagac gtactttgtt tgcaagaagt tcaagaagat cattatggag
241cagagatcag gccaagtttg gaatcactgg gttatcactg tgaatataag atgcggacag
301gaaggaaacc tgatggctgt gctatttgct tcaaacattc caaattttca ctcttgtcag
361tgaacccagt ggaattcttc cgccctgata tttctctgtt ggacagagac aatgttggat
421tagttttact cttacagccc aaaattccat atgctgcctg ccctgcaatc tgcgtagcaa
481atacgcatct gttgtataat cCAAGGCGAG GTGATATTAA GCTGACGCAA TTGGCAATGC
541TACTGGCAGA GATTTCCAGT GTTGCCCACC AGAAAGATGG CAGCTTCTGC CCTATTGTTA
601TGTGTGGTGA CTTTAATTCT GTTCCTGGTT CTCCACTATA TAGTTTCATA AAGGAAGGAA
661AATTGAATTA TGAAGGACTT CCCATAGGAA AGGTATCTGG CCAGGAACAG TCTTCACGGG
721GACAAAGAAT TTTATCTATT CCAATTTGGC CCCCAAACCT AGGTATCTCA CAGAACTGTG
781TGTATGAGGT ACAGCAGGTA CCAAAAGTAG AAAAGACAGA CAGTGATCTG ACACAAACAC
841AGCTGAAGCA AACAGAGGTC CTAGTGACAG CTGAAAAATT GTCTTCAAAT TTACAGCACC
901ATTTCAGTTT GTCATCTGTT TATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
961AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1021TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GCCCGCTCAC
1081ATCTTTTAGG TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1141att

Download FASTA (ORF) (Full)