Construct: ORF TRCN0000465673
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014260.1_s317c1
- Derived from:
- ccsbBroadEn_03283
- DNA Barcode:
- AAAGGGTATCAGTCACCATAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSRC1 (51319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465673
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51319 | RSRC1 | arginine and serine rich co... | NM_001271838.2 | 100% | 100% | |
| 2 | human | 51319 | RSRC1 | arginine and serine rich co... | NM_016625.3 | 100% | 100% | |
| 3 | human | 51319 | RSRC1 | arginine and serine rich co... | NM_001271834.1 | 82.6% | 82.6% | 319_320ins174 |
| 4 | human | 51319 | RSRC1 | arginine and serine rich co... | XM_024453560.1 | 68.8% | 63.7% | (many diffs) |
| 5 | human | 51319 | RSRC1 | arginine and serine rich co... | XM_024453562.1 | 51.4% | 46.7% | (many diffs) |
| 6 | human | 51319 | RSRC1 | arginine and serine rich co... | XM_024453563.1 | 51.4% | 46.7% | (many diffs) |
| 7 | human | 51319 | RSRC1 | arginine and serine rich co... | XR_002959540.1 | 13.3% | (many diffs) | |
| 8 | mouse | 66880 | Rsrc1 | arginine/serine-rich coiled... | NM_025822.3 | 86.8% | 91.9% | (many diffs) |
| 9 | mouse | 66880 | Rsrc1 | arginine/serine-rich coiled... | XM_017319695.1 | 85.7% | 90.8% | (many diffs) |
| 10 | mouse | 66880 | Rsrc1 | arginine/serine-rich coiled... | XM_017319692.1 | 57% | 60.4% | (many diffs) |
| 11 | mouse | 66880 | Rsrc1 | arginine/serine-rich coiled... | XM_017319693.1 | 57% | 60.4% | (many diffs) |
| 12 | mouse | 66880 | Rsrc1 | arginine/serine-rich coiled... | XM_017319694.1 | 57% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1068
- ORF length:
- 1002
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acgtcggtca tcagatactg aagaagaaag cagaagcaag agaaaaaaga 121 aacaccgtag acggtcctcc tcgagcagtt cttcagatag tagaacatac agccgaaaga 181 aaggaggaag gaaatcaaga tcaaagtcaa gatcttggtc cagagatctt cagcctcgtt 241 cacattctta tgatagaaga cgcaggcatc gatcaagcag tagctcttct tatggctcca 301 gaaggaaacg aagtcgaagt cgttcaaggg gtcgagggaa atcctataga gttcagaggt 361 ctaggtcaaa aagcagaaca agaaggtcca ggtcaagacc tcgtctccgt tctcatagtc 421 gtagcagtga aaggtccagt cacagaagaa cgcgtagtcg gtctcgggat agagaacgac 481 gtaagggcag agataaagag aaaagagaaa aggagaagga taaagggaag gacaaggaat 541 tacataacat caaacgtggg gaatctggaa acatcaaagc tggattagaa catctgccac 601 cagctgaaca ggccaaagcc agactacagc tggttcttga agctgctgca aaagctgatg 661 aagcattgaa agccaaagaa agaaatgagg aagaagcaaa gagaagaaag gaggaagacc 721 aagccaccct ggtagaacaa gtaaaaagag taaaagaaat tgaagctatt gaaagtgatT 781 CTTTTGTTCA GCAGACATTC AGATCAAGTA AAGAAGTCAA AAAGTCAGTG GAACCTAGTG 841 AAGTGAAACA AGCAACTTCA ACATCAGGAC CAGCATCAGC AGTTGCTGAT CCACCCAGTA 901 CTGAAAAAGA AATAGATCCT ACCAGCATCC CTACTGCTAT CAAGTACCAA GATGACAATT 961 CCCTGGCCCA TCCAAATTTA TTTATCGAGA AAGCTGATGC TGAGGAAAAA TGGTTCAAGA 1021 GATTAATTGC TCTCCGACAA GAAAGACTAA TGGGCAGTCC TGTGGCCTAC CCAACTTTCT 1081 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1141 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1201 GTGGAAAGGA CGAAAAGGGT ATCAGTCACC ATAACAAACG CGTTAAGTCg acaatcaacc 1261 tctggattac aaaatttgtg aaagatt