Construct: ORF TRCN0000466902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007708.1_s317c1
- Derived from:
- ccsbBroadEn_13398
- DNA Barcode:
- ACCACAGTACACCCTTGCTTCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBB (203068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203068 | TUBB | tubulin beta class I | NM_001293213.2 | 51.1% | 50% | 1_344delinsA;350_352delTGG;355_362delGTGGTACG |
| 2 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519460.2 | 35.4% | 36.9% | (many diffs) |
| 3 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303529.2 | 34.6% | 36% | (many diffs) |
| 4 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303530.2 | 34.6% | 36% | (many diffs) |
| 5 | human | 203068 | TUBB | tubulin beta class I | NM_001293215.2 | 33.3% | 33.3% | 1_744del |
| 6 | human | 203068 | TUBB | tubulin beta class I | NM_001293216.2 | 33.3% | 33.3% | 1_744del |
| 7 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016192.2 | 31.1% | 32.4% | (many diffs) |
| 8 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016193.2 | 31.1% | 32.4% | (many diffs) |
| 9 | human | 203068 | TUBB | tubulin beta class I | NM_001293214.2 | 31% | 31% | 1_828del |
| 10 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001310315.2 | 30.1% | 31.9% | (many diffs) |
| 11 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289130.1 | 28.9% | 31.3% | (many diffs) |
| 12 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289131.1 | 28.9% | 31.3% | (many diffs) |
| 13 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_001197181.2 | 27.9% | 29.3% | (many diffs) |
| 14 | human | 203068 | TUBB | tubulin beta class I | NM_178014.4 | 27.9% | 27.9% | 1_960del |
| 15 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303527.2 | 27.8% | 28.8% | (many diffs) |
| 16 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519459.3 | 27.8% | 28.9% | (many diffs) |
| 17 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303528.2 | 27.3% | 28.3% | (many diffs) |
| 18 | human | 203068 | TUBB | tubulin beta class I | NM_001293212.2 | 26.7% | 26.7% | 1_1020del |
| 19 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303526.2 | 25.4% | 26.4% | (many diffs) |
| 20 | human | 10383 | TUBB4B | tubulin beta 4B class IVb | NM_006088.6 | 24.4% | 26.5% | (many diffs) |
| 21 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001069.3 | 24.4% | 25.8% | (many diffs) |
| 22 | human | 347733 | TUBB2B | tubulin beta 2B class IIb | NM_178012.5 | 24.4% | 25.8% | (many diffs) |
| 23 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289129.1 | 24.2% | 26.2% | (many diffs) |
| 24 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_006087.4 | 24.2% | 26.2% | (many diffs) |
| 25 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_006086.4 | 23.5% | 24.6% | (many diffs) |
| 26 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303524.1 | 23.3% | 24.2% | (many diffs) |
| 27 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_032525.3 | 23.3% | 24.2% | (many diffs) |
| 28 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | NM_177987.2 | 23.3% | 24.2% | (many diffs) |
| 29 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289127.1 | 22% | 23.8% | (many diffs) |
| 30 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289123.1 | 21.7% | 23.5% | (many diffs) |
| 31 | human | 203068 | TUBB | tubulin beta class I | NR_120608.2 | 19.4% | 1_516del;889_1916del | |
| 32 | mouse | 22154 | Tubb5 | tubulin, beta 5 class I | NM_011655.5 | 26.2% | 27.9% | (many diffs) |
| 33 | mouse | 22151 | Tubb2a | tubulin, beta 2A class IIA | NM_009450.2 | 25.1% | 25.8% | (many diffs) |
| 34 | mouse | 73710 | Tubb2b | tubulin, beta 2B class IIB | NM_023716.2 | 25% | 25.8% | (many diffs) |
| 35 | mouse | 22153 | Tubb4a | tubulin, beta 4A class IVA | NM_009451.3 | 24.4% | 26.2% | (many diffs) |
| 36 | mouse | 67951 | Tubb6 | tubulin, beta 6 class V | NM_026473.2 | 23.9% | 24.6% | (many diffs) |
| 37 | mouse | 22152 | Tubb3 | tubulin, beta 3 class III | NM_023279.2 | 23.8% | 24.4% | (many diffs) |
| 38 | mouse | 227613 | Tubb4b | tubulin, beta 4B class IVB | NM_146116.2 | 23.8% | 26.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 438
- ORF length:
- 372
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC CATGAAGGAG GTCGATGAGC AGATGCTTAA CGTGCAGAAC AAGAACAGCA 121 GCTACTTTGT GGAATGGATC CCCAACAATG TCAAGACAGC CGTCTGTGAC ATCCCACCTC 181 GTGGCCTCAA GATGGCAGTC ACCTTCATTG GCAATAGCAC AGCCATCCAG GAGCTCTTCA 241 AGCGCATCTC GGAGCAGTTC ACTGCCATGT TCCGCCGGAA GGCCTTCCTC CACTGGTACA 301 CAGGCGAGGG CATGGACGAG ATGGAGTTCA CCGAGGCTGA GAGCAACATG AACGACCTCG 361 TCTCTGAGTA TCAGCAGTAC CAGGATGCCA CCGCAGAAGA GGAGGAGGAT TTCGGTGAGG 421 AGGCCGAAGA GGAGGCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCACAG TACACCCTTG 601 CTTCGCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt