Construct: ORF TRCN0000468350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011437.2_s317c1
- Derived from:
- ccsbBroadEn_15118
- DNA Barcode:
- TATCTGAATCAGCCTGATTTTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK1D (57118)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_153498.4 | 99.9% | 100% | 948C>G |
| 2 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519591.3 | 94.6% | 89.6% | (many diffs) |
| 3 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_006717482.3 | 93.9% | 86.1% | 948C>G;995_996ins43;1086_1087ins26 |
| 4 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_006717483.4 | 91.4% | 90.6% | 948C>G;1040_1043delGTGC;1065_1066ins94 |
| 5 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_020397.4 | 90.8% | 90.3% | (many diffs) |
| 6 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519592.3 | 81.1% | 80.3% | (many diffs) |
| 7 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519593.3 | 80.6% | 80.5% | 0_1ins222;2T>A;726C>G |
| 8 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519595.3 | 74.7% | 74.8% | 0_1ins291;657C>G |
| 9 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_017016438.2 | 74.7% | 74.8% | 0_1ins291;657C>G |
| 10 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_024448087.1 | 74.7% | 74.8% | 0_1ins291;657C>G |
| 11 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_001351032.2 | 65.8% | 65.1% | (many diffs) |
| 12 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519594.3 | 64.3% | 64.4% | 0_1ins411;537C>G |
| 13 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_177343.4 | 90.6% | 97.6% | (many diffs) |
| 14 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_011238973.2 | 85.6% | 89% | (many diffs) |
| 15 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290375.1 | 85.3% | 85% | (many diffs) |
| 16 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290376.1 | 85.3% | 87.4% | (many diffs) |
| 17 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497470.3 | 83% | 89.6% | (many diffs) |
| 18 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497469.3 | 82.7% | 89.6% | (many diffs) |
| 19 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497471.3 | 80.7% | 86.2% | (many diffs) |
| 20 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290374.1 | 79.3% | 78.4% | (many diffs) |
| 21 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497473.3 | 58.5% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgggagaac ggcgagagca gctcctcctg gaaaaagcaa gctgaagaca 121 tcaagaagat cttcgagttc aaagagaccc tcggaaccgg ggccttttcc gaagtggttt 181 tagctgaaga gaaggcaact ggcaagctct ttgctgtgaa gtgtatccct aagaaggcgc 241 tgaagggcaa ggaaagcagc atagagaatg agatagccgt cctgagaaag attaagcatg 301 aaaatattgt tgccctggaa gacatttatg aaagcccaaa tcacctgtac ttggtcatgc 361 agctggtgtc cggtggagag ctgtttgacc ggatagtgga gaaggggttt tatacagaga 421 aggatgccag cactctgatc cgccaagtct tggacgccgt gtactatctc cacagaatgg 481 gcatcgtcca cagagacctc aagcccgaaa atctcttgta ctacagtcaa gatgaggagt 541 ccaaaataat gatcagtgac tttggattgt caaaaatgga gggcaaagga gatgtgatgt 601 ccactgcctg tggaactcca ggctatgtcg ctcctgaagt cctcgcccag aaaccttaca 661 gcaaagccgt tgactgctgg tccatcggag tgattgccta catcttgctc tgcggctacc 721 ctccttttta tgatgaaaat gactccaagc tctttgagca gatcctcaag gcggaatatg 781 agtttgactc tccctactgg gatgacatct ccgactctgc aaaagacttc attcggaacc 841 TGATGGAGAA GGACCCGAAT AAAAGATACA CGTGTGAGCA GGCAGCTCGG CACCCATGGA 901 TCGCTGGTGA CACAGCCCTC AACAAAAACA TCCACGAGTC CGTCAGCGCC CAGATCCGGA 961 AAAACTTTGC CAAGAGCAAA TGGAGACAAG CATTTAATGC CACGGCCGTC GTGAGACATA 1021 TGAGAAAACT ACACCTCGGC AGCAGCCTGG ACAGTTCAAA TGCAAGTGTT TCGAGCAGCC 1081 TCAGTTTGGC CAGCCAAAAA GACTGTCTGG CACCTTCCAC GCTCTGTAGT TTCATTTCTT 1141 CTTCGTCGGG GGTCTCAGGA GTTGGAGCCG AGCGGAGACC CAGGCCCACC ACTGTGACGG 1201 CAGTGCACTC TGGAAGCAAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATC TGAATCAGCC 1381 TGATTTTGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt