Transcript: Human XM_017016438.2

PREDICTED: Homo sapiens calcium/calmodulin dependent protein kinase ID (CAMK1D), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CAMK1D (57118)
Length:
2231
CDS:
863..1729

Additional Resources:

NCBI RefSeq record:
XM_017016438.2
NBCI Gene record:
CAMK1D (57118)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017016438.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000197282 GCCGTCCTGAGAAAGATTAAG pLKO.1 782 5UTR 100% 13.200 18.480 N CAMK1D n/a
2 TRCN0000352651 GCCGTCCTGAGAAAGATTAAG pLKO_005 782 5UTR 100% 13.200 18.480 N CAMK1D n/a
3 TRCN0000195531 CGGAGTGATTGCCTACATCTT pLKO.1 1192 CDS 100% 4.950 6.930 N CAMK1D n/a
4 TRCN0000342579 CGGAGTGATTGCCTACATCTT pLKO_005 1192 CDS 100% 4.950 6.930 N CAMK1D n/a
5 TRCN0000195715 CAGATCCTCAAGGCGGAATAT pLKO.1 1265 CDS 100% 13.200 9.240 N CAMK1D n/a
6 TRCN0000001756 TGATGGAGAAGGACCCGAATA pLKO.1 1347 CDS 100% 10.800 7.560 N CAMK1D n/a
7 TRCN0000342628 TGATGGAGAAGGACCCGAATA pLKO_005 1347 CDS 100% 10.800 7.560 N CAMK1D n/a
8 TRCN0000001755 AGAGCTGTTTGACCGGATAGT pLKO.1 883 CDS 100% 4.950 3.465 N CAMK1D n/a
9 TRCN0000342578 AGAGCTGTTTGACCGGATAGT pLKO_005 883 CDS 100% 4.950 3.465 N CAMK1D n/a
10 TRCN0000197125 GACTTCATTCGGAACCTGATG pLKO.1 1331 CDS 100% 4.050 2.835 N CAMK1D n/a
11 TRCN0000001752 TGCTGTGAAGTGTATCCCTAA pLKO.1 718 5UTR 100% 4.050 2.835 N CAMK1D n/a
12 TRCN0000001754 AGGCGGAATATGAGTTTGACT pLKO.1 1275 CDS 100% 3.000 2.100 N CAMK1D n/a
13 TRCN0000342580 AGGCGGAATATGAGTTTGACT pLKO_005 1275 CDS 100% 3.000 2.100 N CAMK1D n/a
14 TRCN0000001753 AGAATGAGATAGCCGTCCTGA pLKO.1 771 5UTR 100% 2.640 1.848 N CAMK1D n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017016438.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08703 pDONR223 100% 74.7% 74.8% None 0_1ins291;657C>G n/a
2 ccsbBroad304_08703 pLX_304 0% 74.7% 74.8% V5 0_1ins291;657C>G n/a
3 TRCN0000472635 AAAGGACCTGACCAGAGAAGTGCC pLX_317 33.2% 74.7% 74.8% V5 0_1ins291;657C>G n/a
4 ccsbBroadEn_15118 pDONR223 0% 74.7% 74.8% None 0_1ins291;657C>G n/a
5 ccsbBroad304_15118 pLX_304 0% 74.7% 74.8% V5 0_1ins291;657C>G n/a
6 TRCN0000468350 TATCTGAATCAGCCTGATTTTGGG pLX_317 33.2% 74.7% 74.8% V5 0_1ins291;657C>G n/a
7 TRCN0000489121 ACCATGACATTCTAAAGTACAACG pLX_317 28.3% 65.9% 64.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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