Construct: ORF TRCN0000468663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013077.1_s317c1
- Derived from:
- ccsbBroadEn_09779
- DNA Barcode:
- TTCTCTACAAAACGCCGTTGCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLIS3 (169792)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | NM_152629.3 | 99.8% | 99.4% | (many diffs) |
| 2 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_005251386.4 | 99.8% | 99.4% | (many diffs) |
| 3 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517766.2 | 99.8% | 99.4% | (many diffs) |
| 4 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_017014361.1 | 99.8% | 99.4% | (many diffs) |
| 5 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_005251387.4 | 91.1% | 90.8% | (many diffs) |
| 6 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_005251388.4 | 91.1% | 90.8% | (many diffs) |
| 7 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517767.3 | 91.1% | 90.8% | (many diffs) |
| 8 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | NM_001042413.2 | 83.1% | 82.9% | (many diffs) |
| 9 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517763.2 | 83.1% | 82.9% | (many diffs) |
| 10 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517764.2 | 83.1% | 82.9% | (many diffs) |
| 11 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517765.2 | 75% | 73.3% | (many diffs) |
| 12 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XR_929206.2 | 46.4% | (many diffs) | |
| 13 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_011517769.2 | 45.7% | 45% | (many diffs) |
| 14 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_005251389.5 | 45.6% | 44% | (many diffs) |
| 15 | human | 169792 | GLIS3 | GLIS family zinc finger 3 | XM_006716731.3 | 44.6% | 44.3% | (many diffs) |
| 16 | mouse | 226075 | Glis3 | GLIS family zinc finger 3 | XM_006526985.3 | 83.9% | 85.1% | (many diffs) |
| 17 | mouse | 226075 | Glis3 | GLIS family zinc finger 3 | NM_001305671.1 | 71% | 72.4% | (many diffs) |
| 18 | mouse | 226075 | Glis3 | GLIS family zinc finger 3 | NM_175459.6 | 70% | 71% | (many diffs) |
| 19 | mouse | 226075 | Glis3 | GLIS family zinc finger 3 | XM_006526984.3 | 70% | 71% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2391
- ORF length:
- 2325
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggttcagcga ctgggactca tttcacctcc agcaagccag gtctctacag 121 catgcaacca gatcagtcct agcttacaga gggcaatgaa tgcagccaac ctgaatatac 181 ctccttcaga taccaggtcc cttatttcgc gtgagtcttt ggcgtccacg accttgagtc 241 tgacggaaag tcagtcggcc tcaagcatga agcaggagtg gtcccagggc tacagggccc 301 tcccttcgct ctccaaccac ggctctcaga atggccttga tctaggggat ctccttagcc 361 ttcctcccgg gacatccatg tccagcaata gtgtctctaa ctcattacca tcctaccttt 421 ttggcacgga aagtagccac tctccttacc ctagtcctcg gcactcatcc accaggtccc 481 actcggcccg ctccaagaag agagcgctgt ccttgtcccc gctgtccgat ggcatcggga 541 tagatttcaa taccatcatc cgcacgtcgc ccacgtcctt ggtggcctac atcaacgggt 601 cgagggcttc gccggccaac ctgtccccgc agccggaggt ctacgggcat ttcctgggcg 661 tgcgcggcag ctgcattccc cagccgcgcc cggtgcccgg cagccagaag ggcgtgctgg 721 tggcccctgg aggcctggcg ctgccggcct acggcgagga cggggccctg gagcacgagc 781 gcatgcaaca gctggagcac ggcggcctgc agccaggcct ggtcaaccac atggtggtgc 841 agcatggcct gccgggcccc gacagccagc cggccggcct gttcaagacc gaacgcctgg 901 aggagttccc gggcagcacc gtagacctac cccccgcgcc tccgctccct cctctgccgc 961 cgccccaagg ccccccaccc ccttaccatg cccatgcgca ccttcaccac ccggagctcg 1021 ggccccacgc ccagcagctg gccttgcccc aggccaccct ggacgacgac ggggagatgg 1081 acggcatcgg gggcaagcat tgctaccgct ggatcgactg cagcgccctg tacgaccagc 1141 aggaggagct cgtgcggcac atcgagaagg tccacatcga ccagcgcaaa ggggaggact 1201 tcacttgctt ctgggccggt tgccctcgaa gatacaagcc cttcaacgcc cgctataaac 1261 tgctgatcca catgagagtc cactctgggg agaagcccaa caagtgtacg tttgaaggtt 1321 gcgagaaggc cttttcaagg cttgaaaatc tcaagatcca cttgcggagc cacacaggcg 1381 agaagccgta tttgtgccag catccgggtt gtcagaaggc cttcagtaac tccagtgacc 1441 gcgccaaaca ccagcggacg catctggaca ccaaacctta tgcttgtcaa attccaggat 1501 gtaccaaacg ctacacagac ccaagttccc taagaaagca tgtgaaggca cattcttcca 1561 aagagcaaca agcaaggaaa aagttgcggt ccagcacaga gctccatcca gacctgctca 1621 cagattgcct caccgtgcag tccctgcagc cggccacttc ccctagagat gctgctgctg 1681 aagggaccgt gggacgctcc cctggacccg ggcctgacct ctattcagct cccattttct 1741 ccagcaatta ttcaagccga agtggaacag ctgctggggc cgtaccaccc ccacatcctg 1801 tcagtcaccc ttctccagga cataatgtac aggggagccc tcacaacccc tcctcccagt 1861 tacctccact cacagctgtg gacgcaggag ctgagaggtt tgcaccttct gctccatctc 1921 ctcaccacat cagcccccgg agagttccag ctccttcTTC AATACTGCAA AGAACACAGC 1981 CTCCCTATAC CCAGCAGCCA TCAGGTTCAC ACCTGAAGTC CTATCAGCCA GAAACAAACT 2041 CTTCTTTTCA ACCAAATGGT ATCCATGTCC ATGGATTTTA TGGGCAGCTG CAGAAGTTCT 2101 GTCCCCCACA CTACCCCGAT TCCCAGAGAA TTGTGCCGCC TGTCAGCTCC TGCAGTGTGG 2161 TGCCTTCGTT TGAGGACTGC CTAGTCCCTA CATCCATGGG CCAGGCCAGT TTTGATGTTT 2221 TCCACAGAGC CTTCTCGACT CACTCGGGCA TTACAGTGTA TGATTTACCT TCAAGTTCCT 2281 CGAGCCTCTT TGGGGAGTCT CTCCGCAGCG GGGCTGAAGA TGCTACCTTC TTGCAGATCA 2341 GCACCGTGGA CCGCTGTCCT AGCCAGCTCT CCTCTGTCTG CACCGAAGGC TACCCAACTT 2401 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2461 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2521 CTTGTGGAAA GGACGATTCT CTACAAAACG CCGTTGCAGT ACGCGTTAAG TCgacaatca 2581 acctctggat tacaaaattt gtgaaagatt