Construct: ORF TRCN0000468663

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013077.1_s317c1
Derived from:
ccsbBroadEn_09779
DNA Barcode:
TTCTCTACAAAACGCCGTTGCAGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GLIS3 (169792)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468663
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 169792 GLIS3 GLIS family zinc finger 3 NM_152629.3 99.8% 99.4% (many diffs)
2 human 169792 GLIS3 GLIS family zinc finger 3 XM_005251386.4 99.8% 99.4% (many diffs)
3 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517766.2 99.8% 99.4% (many diffs)
4 human 169792 GLIS3 GLIS family zinc finger 3 XM_017014361.1 99.8% 99.4% (many diffs)
5 human 169792 GLIS3 GLIS family zinc finger 3 XM_005251387.4 91.1% 90.8% (many diffs)
6 human 169792 GLIS3 GLIS family zinc finger 3 XM_005251388.4 91.1% 90.8% (many diffs)
7 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517767.3 91.1% 90.8% (many diffs)
8 human 169792 GLIS3 GLIS family zinc finger 3 NM_001042413.2 83.1% 82.9% (many diffs)
9 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517763.2 83.1% 82.9% (many diffs)
10 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517764.2 83.1% 82.9% (many diffs)
11 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517765.2 75% 73.3% (many diffs)
12 human 169792 GLIS3 GLIS family zinc finger 3 XR_929206.2 46.4% (many diffs)
13 human 169792 GLIS3 GLIS family zinc finger 3 XM_011517769.2 45.7% 45% (many diffs)
14 human 169792 GLIS3 GLIS family zinc finger 3 XM_005251389.5 45.6% 44% (many diffs)
15 human 169792 GLIS3 GLIS family zinc finger 3 XM_006716731.3 44.6% 44.3% (many diffs)
16 mouse 226075 Glis3 GLIS family zinc finger 3 XM_006526985.3 83.9% 85.1% (many diffs)
17 mouse 226075 Glis3 GLIS family zinc finger 3 NM_001305671.1 71% 72.4% (many diffs)
18 mouse 226075 Glis3 GLIS family zinc finger 3 NM_175459.6 70% 71% (many diffs)
19 mouse 226075 Glis3 GLIS family zinc finger 3 XM_006526984.3 70% 71% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2391
ORF length:
2325
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat ggttcagcga ctgggactca tttcacctcc agcaagccag gtctctacag
121catgcaacca gatcagtcct agcttacaga gggcaatgaa tgcagccaac ctgaatatac
181ctccttcaga taccaggtcc cttatttcgc gtgagtcttt ggcgtccacg accttgagtc
241tgacggaaag tcagtcggcc tcaagcatga agcaggagtg gtcccagggc tacagggccc
301tcccttcgct ctccaaccac ggctctcaga atggccttga tctaggggat ctccttagcc
361ttcctcccgg gacatccatg tccagcaata gtgtctctaa ctcattacca tcctaccttt
421ttggcacgga aagtagccac tctccttacc ctagtcctcg gcactcatcc accaggtccc
481actcggcccg ctccaagaag agagcgctgt ccttgtcccc gctgtccgat ggcatcggga
541tagatttcaa taccatcatc cgcacgtcgc ccacgtcctt ggtggcctac atcaacgggt
601cgagggcttc gccggccaac ctgtccccgc agccggaggt ctacgggcat ttcctgggcg
661tgcgcggcag ctgcattccc cagccgcgcc cggtgcccgg cagccagaag ggcgtgctgg
721tggcccctgg aggcctggcg ctgccggcct acggcgagga cggggccctg gagcacgagc
781gcatgcaaca gctggagcac ggcggcctgc agccaggcct ggtcaaccac atggtggtgc
841agcatggcct gccgggcccc gacagccagc cggccggcct gttcaagacc gaacgcctgg
901aggagttccc gggcagcacc gtagacctac cccccgcgcc tccgctccct cctctgccgc
961cgccccaagg ccccccaccc ccttaccatg cccatgcgca ccttcaccac ccggagctcg
1021ggccccacgc ccagcagctg gccttgcccc aggccaccct ggacgacgac ggggagatgg
1081acggcatcgg gggcaagcat tgctaccgct ggatcgactg cagcgccctg tacgaccagc
1141aggaggagct cgtgcggcac atcgagaagg tccacatcga ccagcgcaaa ggggaggact
1201tcacttgctt ctgggccggt tgccctcgaa gatacaagcc cttcaacgcc cgctataaac
1261tgctgatcca catgagagtc cactctgggg agaagcccaa caagtgtacg tttgaaggtt
1321gcgagaaggc cttttcaagg cttgaaaatc tcaagatcca cttgcggagc cacacaggcg
1381agaagccgta tttgtgccag catccgggtt gtcagaaggc cttcagtaac tccagtgacc
1441gcgccaaaca ccagcggacg catctggaca ccaaacctta tgcttgtcaa attccaggat
1501gtaccaaacg ctacacagac ccaagttccc taagaaagca tgtgaaggca cattcttcca
1561aagagcaaca agcaaggaaa aagttgcggt ccagcacaga gctccatcca gacctgctca
1621cagattgcct caccgtgcag tccctgcagc cggccacttc ccctagagat gctgctgctg
1681aagggaccgt gggacgctcc cctggacccg ggcctgacct ctattcagct cccattttct
1741ccagcaatta ttcaagccga agtggaacag ctgctggggc cgtaccaccc ccacatcctg
1801tcagtcaccc ttctccagga cataatgtac aggggagccc tcacaacccc tcctcccagt
1861tacctccact cacagctgtg gacgcaggag ctgagaggtt tgcaccttct gctccatctc
1921ctcaccacat cagcccccgg agagttccag ctccttcTTC AATACTGCAA AGAACACAGC
1981CTCCCTATAC CCAGCAGCCA TCAGGTTCAC ACCTGAAGTC CTATCAGCCA GAAACAAACT
2041CTTCTTTTCA ACCAAATGGT ATCCATGTCC ATGGATTTTA TGGGCAGCTG CAGAAGTTCT
2101GTCCCCCACA CTACCCCGAT TCCCAGAGAA TTGTGCCGCC TGTCAGCTCC TGCAGTGTGG
2161TGCCTTCGTT TGAGGACTGC CTAGTCCCTA CATCCATGGG CCAGGCCAGT TTTGATGTTT
2221TCCACAGAGC CTTCTCGACT CACTCGGGCA TTACAGTGTA TGATTTACCT TCAAGTTCCT
2281CGAGCCTCTT TGGGGAGTCT CTCCGCAGCG GGGCTGAAGA TGCTACCTTC TTGCAGATCA
2341GCACCGTGGA CCGCTGTCCT AGCCAGCTCT CCTCTGTCTG CACCGAAGGC TACCCAACTT
2401TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2461CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2521CTTGTGGAAA GGACGATTCT CTACAAAACG CCGTTGCAGT ACGCGTTAAG TCgacaatca
2581acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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