Construct: ORF TRCN0000469377
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005867.1_s317c1
- Derived from:
- ccsbBroadEn_01376
- DNA Barcode:
- CGCATTGTATTTCGACTGGATGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RARB (5915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469377
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5915 | RARB | retinoic acid receptor beta | NM_000965.4 | 100% | 100% | |
| 2 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290216.2 | 90.6% | 84.8% | (many diffs) |
| 3 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290277.1 | 89.7% | 89.7% | 785_786ins138 |
| 4 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290300.1 | 89.5% | 88.3% | (many diffs) |
| 5 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290266.1 | 88.6% | 88.1% | 0_1ins148;4_6delGAAinsCTC;10delG |
| 6 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290217.1 | 75% | 75% | 0_1ins336 |
| 7 | human | 5915 | RARB | retinoic acid receptor beta | NM_001290276.1 | 75% | 75% | 0_1ins336 |
| 8 | human | 5915 | RARB | retinoic acid receptor beta | NM_016152.3 | 75% | 75% | 0_1ins336 |
| 9 | human | 5915 | RARB | retinoic acid receptor beta | NR_110892.1 | 36.7% | 1_469del;917_918ins161;1653_3061del | |
| 10 | human | 5915 | RARB | retinoic acid receptor beta | NR_110893.1 | 35.3% | 1_469del;1254_1255ins205;1609_3017del | |
| 11 | mouse | 218772 | Rarb | retinoic acid receptor, beta | NM_011243.2 | 91% | 98.4% | (many diffs) |
| 12 | mouse | 218772 | Rarb | retinoic acid receptor, beta | NM_001289761.1 | 81.5% | 84% | (many diffs) |
| 13 | mouse | 218772 | Rarb | retinoic acid receptor, beta | NM_001289762.1 | 80.9% | 87.2% | (many diffs) |
| 14 | mouse | 218772 | Rarb | retinoic acid receptor, beta | NM_001289760.1 | 79.8% | 84% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgactgtatg gatgttctgt cagtgagtcc tgggcaaatc ctggatttct 121 acactgcgag tccgtcttcc tgcatgctcc aggagaaagc tctcaaagca tgcttcagtg 181 gattgaccca aaccgaatgg cagcatcggc acactgctca atcaattgaa acacagagca 241 ccagctctga ggaactcgtc ccaagccccc catctccact tcctccccct cgagtgtaca 301 aaccctgctt cgtctgccag gacaaatcat cagggtacca ctatggggtc agcgcctgtg 361 agggatgtaa gggctttttc cgcagaagta ttcagaagaa tatgatttac acttgtcacc 421 gagataagaa ctgtgttatt aataaagtca ccaggaatcg atgccaatac tgtcgactcc 481 agaagtgctt tgaagtggga atgtccaaag aatctgtcag gaatgacagg aacaagaaaa 541 agaaggagac ttcgaagcaa gaatgcacag agagctatga aatgacagct gagttggacg 601 atctcacaga gaagatccga aaagctcacc aggaaacttt cccttcactc tgccagctgg 661 gtaaatacac cacgaattcc agtgctgacc atcgagtccg actggacctg ggcctctggg 721 acaaattcag tgaactggcc accaagtgca ttattaagat cgtggagttt gctaaacgtc 781 tgcctggttt cactggcttg accatcgcag accaaattac cctgctgaag gccgcctgcc 841 tggacatcct gattcttaga atttgcacca ggtatacccc agaacaagac accatgactt 901 tctcagacgg ccttacccta aatcgaactc agatgcacaa tgctggattt ggtccTCTGA 961 CTGACCTTGT GTTCACCTTT GCCAACCAGC TCCTGCCTTT GGAAATGGAT GACACAGAAA 1021 CAGGCCTTCT CAGTGCCATC TGCTTAATCT GTGGAGACCG CCAGGACCTT GAGGAACCGA 1081 CAAAAGTAGA TAAGCTACAA GAACCATTGC TGGAAGCACT AAAAATTTAT ATCAGAAAAA 1141 GACGACCCAG CAAGCCTCAC ATGTTTCCAA AGATCTTAAT GAAAATCACA GATCTCCGTA 1201 GCATCAGTGC TAAAGGTGCA GAGCGTGTAA TTACCTTGAA AATGGAAATT CCTGGATCAA 1261 TGCCACCTCT CATTCAAGAA ATGCTGGAGA ATTCTGAAGG ACATGAACCC TTGACCCCAA 1321 GTTCAAGTGG GAACACAGCA GAGCACAGTC CTAGCATCTC ACCCAGCTCA GTGGAAAACA 1381 GTGGGGTCAG TCAGTCACCA CTCGTGCAAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCAT 1561 TGTATTTCGA CTGGATGGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt