Construct: ORF TRCN0000469802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006897.1_s317c1
- Derived from:
- ccsbBroadEn_02415
- DNA Barcode:
- TTTCCTGATTTATTTGACTTAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBB3 (10381)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469802
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_006086.4 | 100% | 100% | |
| 2 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001069.3 | 87.8% | 91.1% | (many diffs) |
| 3 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289129.1 | 87.8% | 90.8% | (many diffs) |
| 4 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_006087.4 | 87.8% | 90.8% | (many diffs) |
| 5 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303524.1 | 87.7% | 91.5% | (many diffs) |
| 6 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_032525.3 | 87.7% | 91.5% | (many diffs) |
| 7 | human | 347733 | TUBB2B | tubulin beta 2B class IIb | NM_178012.5 | 87.3% | 91.3% | (many diffs) |
| 8 | human | 10383 | TUBB4B | tubulin beta 4B class IVb | NM_006088.6 | 84% | 91.7% | (many diffs) |
| 9 | human | 10381 | TUBB3 | tubulin beta 3 class III | NM_001197181.2 | 84% | 84% | 0_1ins216 |
| 10 | human | 203068 | TUBB | tubulin beta class I | NM_178014.4 | 81.8% | 91.3% | (many diffs) |
| 11 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303526.2 | 81.8% | 84% | (many diffs) |
| 12 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | NM_177987.2 | 80% | 85.3% | (many diffs) |
| 13 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289127.1 | 79.8% | 82.6% | (many diffs) |
| 14 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289123.1 | 78.9% | 81.6% | (many diffs) |
| 15 | human | 203068 | TUBB | tubulin beta class I | NM_001293212.2 | 77.3% | 84.4% | (many diffs) |
| 16 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289130.1 | 75.1% | 77.1% | (many diffs) |
| 17 | human | 10382 | TUBB4A | tubulin beta 4A class IVa | NM_001289131.1 | 75.1% | 77.1% | (many diffs) |
| 18 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303527.2 | 74.7% | 77.3% | (many diffs) |
| 19 | human | 203068 | TUBB | tubulin beta class I | NM_001293214.2 | 74.2% | 83.1% | (many diffs) |
| 20 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303528.2 | 74% | 71.9% | (many diffs) |
| 21 | human | 7280 | TUBB2A | tubulin beta 2A class IIa | NM_001310315.2 | 72.7% | 73% | (many diffs) |
| 22 | human | 203068 | TUBB | tubulin beta class I | NM_001293215.2 | 68.9% | 76.8% | (many diffs) |
| 23 | human | 203068 | TUBB | tubulin beta class I | NM_001293216.2 | 68.9% | 76.8% | (many diffs) |
| 24 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_011519459.3 | 68.1% | 72.5% | (many diffs) |
| 25 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451145.1 | 67.6% | 71.4% | (many diffs) |
| 26 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303529.2 | 60.8% | 62% | (many diffs) |
| 27 | human | 84617 | TUBB6 | tubulin beta 6 class V | NM_001303530.2 | 60.8% | 62% | (many diffs) |
| 28 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016192.2 | 60.5% | 64.6% | (many diffs) |
| 29 | human | 347688 | TUBB8 | tubulin beta 8 class VIII | XM_017016193.2 | 60.5% | 64.6% | (many diffs) |
| 30 | human | 260334 | TUBB8B | tubulin beta 8B | XM_024451147.1 | 59.9% | 63.7% | (many diffs) |
| 31 | human | 203068 | TUBB | tubulin beta class I | NM_001293213.2 | 44.9% | 48.4% | (many diffs) |
| 32 | mouse | 22152 | Tubb3 | tubulin, beta 3 class III | NM_023279.2 | 90.7% | 99.7% | (many diffs) |
| 33 | mouse | 67951 | Tubb6 | tubulin, beta 6 class V | NM_026473.2 | 86.8% | 91.7% | (many diffs) |
| 34 | mouse | 73710 | Tubb2b | tubulin, beta 2B class IIB | NM_023716.2 | 84.2% | 91.3% | (many diffs) |
| 35 | mouse | 22151 | Tubb2a | tubulin, beta 2A class IIA | NM_009450.2 | 83.6% | 91.1% | (many diffs) |
| 36 | mouse | 22153 | Tubb4a | tubulin, beta 4A class IVA | NM_009451.3 | 82.9% | 90.8% | (many diffs) |
| 37 | mouse | 22154 | Tubb5 | tubulin, beta 5 class I | NM_011655.5 | 82.6% | 91.3% | (many diffs) |
| 38 | mouse | 227613 | Tubb4b | tubulin, beta 4B class IVB | NM_146116.2 | 81.7% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggagatcgtg cacatccagg ccggccagtg cggcaaccag atcggggcca 121 agttctggga agtcatcagt gatgagcatg gcatcgaccc cagcggcaac tacgtgggcg 181 actcggactt gcagctggag cggatcagcg tctactacaa cgaggcctct tctcacaagt 241 acgtgcctcg agccattctg gtggacctgg aacccggaac catggacagt gtccgctcag 301 gggcctttgg acatctcttc aggcctgaca atttcatctt tggtcagagt ggggccggca 361 acaactgggc caagggtcac tacacggagg gggcggagct ggtggattcg gtcctggatg 421 tggtgcggaa ggagtgtgaa aactgcgact gcctgcaggg cttccagctg acccactcgc 481 tggggggcgg cacgggctcc ggcatgggca cgttgctcat cagcaaggtg cgtgaggagt 541 atcccgaccg catcatgaac accttcagcg tcgtgccctc acccaaggtg tcagacacgg 601 tggtggagcc ctacaacgcc acgctgtcca tccaccagct ggtggagaac acggatgaga 661 cctactgcat cgacaacgag gcgctctacg acatctgctt ccgcaccctc aagctggcca 721 cgcccaccta cggggacctc aaccacctgg tatcggccac catgagcgga gtcaccacct 781 ccttgcgctt cccgggccag ctcaacgctg acctgcgcaa gctggccgtc aacatggtgc 841 ccttcccgcg cctgcacttc ttcatgcccg gcttcgcccc cctcacagcc cggggcagcc 901 agcagtaccg ggccctgacc gtgcccgagc tcacccagca gatgttcgat gccaagaaca 961 tgatggccgc cTGCGACCCG CGCCACGGCC GCTACCTGAC GGTGGCCACC GTGTTCCGGG 1021 GCCGCATGTC CATGAAGGAG GTGGACGAGC AGATGCTGGC CATCCAGAGC AAGAACAGCA 1081 GCTACTTCGT GGAGTGGATC CCCAACAACG TGAAGGTGGC CGTGTGTGAC ATCCCGCCCC 1141 GCGGCCTCAA GATGTCCTCC ACCTTCATCG GGAACAGCAC GGCCATCCAG GAGCTGTTCA 1201 AGCGCATCTC CGAGCAGTTC ACGGCCATGT TCCGGCGCAA GGCCTTCCTG CACTGGTACA 1261 CGGGCGAGGG CATGGACGAG ATGGAGTTCA CCGAGGCCGA GAGCAACATG AACGACCTGG 1321 TGTCCGAGTA CCAGCAGTAC CAGGACGCCA CGGCCGAGGA AGAGGGCGAG ATGTACGAAG 1381 ACGACGAGGA GGAGTCGGAG GCCCAGGGCC CCAAGTGCCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 ATTTCCTGAT TTATTTGACT TAATTACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt