Construct: ORF TRCN0000469802

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006897.1_s317c1
Derived from:
ccsbBroadEn_02415
DNA Barcode:
TTTCCTGATTTATTTGACTTAATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB3 (10381)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469802
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 100% 100%
2 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 87.8% 91.1% (many diffs)
3 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 87.8% 90.8% (many diffs)
4 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 87.8% 90.8% (many diffs)
5 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 87.7% 91.5% (many diffs)
6 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 87.7% 91.5% (many diffs)
7 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 87.3% 91.3% (many diffs)
8 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 84% 91.7% (many diffs)
9 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 84% 84% 0_1ins216
10 human 203068 TUBB tubulin beta class I NM_178014.4 81.8% 91.3% (many diffs)
11 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 81.8% 84% (many diffs)
12 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 80% 85.3% (many diffs)
13 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 79.8% 82.6% (many diffs)
14 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 78.9% 81.6% (many diffs)
15 human 203068 TUBB tubulin beta class I NM_001293212.2 77.3% 84.4% (many diffs)
16 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 75.1% 77.1% (many diffs)
17 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 75.1% 77.1% (many diffs)
18 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 74.7% 77.3% (many diffs)
19 human 203068 TUBB tubulin beta class I NM_001293214.2 74.2% 83.1% (many diffs)
20 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 74% 71.9% (many diffs)
21 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 72.7% 73% (many diffs)
22 human 203068 TUBB tubulin beta class I NM_001293215.2 68.9% 76.8% (many diffs)
23 human 203068 TUBB tubulin beta class I NM_001293216.2 68.9% 76.8% (many diffs)
24 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 68.1% 72.5% (many diffs)
25 human 260334 TUBB8B tubulin beta 8B XM_024451145.1 67.6% 71.4% (many diffs)
26 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 60.8% 62% (many diffs)
27 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 60.8% 62% (many diffs)
28 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 60.5% 64.6% (many diffs)
29 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 60.5% 64.6% (many diffs)
30 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 59.9% 63.7% (many diffs)
31 human 203068 TUBB tubulin beta class I NM_001293213.2 44.9% 48.4% (many diffs)
32 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 90.7% 99.7% (many diffs)
33 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 86.8% 91.7% (many diffs)
34 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 84.2% 91.3% (many diffs)
35 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 83.6% 91.1% (many diffs)
36 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 82.9% 90.8% (many diffs)
37 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 82.6% 91.3% (many diffs)
38 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 81.7% 91.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1416
ORF length:
1350
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ggagatcgtg cacatccagg ccggccagtg cggcaaccag atcggggcca
121agttctggga agtcatcagt gatgagcatg gcatcgaccc cagcggcaac tacgtgggcg
181actcggactt gcagctggag cggatcagcg tctactacaa cgaggcctct tctcacaagt
241acgtgcctcg agccattctg gtggacctgg aacccggaac catggacagt gtccgctcag
301gggcctttgg acatctcttc aggcctgaca atttcatctt tggtcagagt ggggccggca
361acaactgggc caagggtcac tacacggagg gggcggagct ggtggattcg gtcctggatg
421tggtgcggaa ggagtgtgaa aactgcgact gcctgcaggg cttccagctg acccactcgc
481tggggggcgg cacgggctcc ggcatgggca cgttgctcat cagcaaggtg cgtgaggagt
541atcccgaccg catcatgaac accttcagcg tcgtgccctc acccaaggtg tcagacacgg
601tggtggagcc ctacaacgcc acgctgtcca tccaccagct ggtggagaac acggatgaga
661cctactgcat cgacaacgag gcgctctacg acatctgctt ccgcaccctc aagctggcca
721cgcccaccta cggggacctc aaccacctgg tatcggccac catgagcgga gtcaccacct
781ccttgcgctt cccgggccag ctcaacgctg acctgcgcaa gctggccgtc aacatggtgc
841ccttcccgcg cctgcacttc ttcatgcccg gcttcgcccc cctcacagcc cggggcagcc
901agcagtaccg ggccctgacc gtgcccgagc tcacccagca gatgttcgat gccaagaaca
961tgatggccgc cTGCGACCCG CGCCACGGCC GCTACCTGAC GGTGGCCACC GTGTTCCGGG
1021GCCGCATGTC CATGAAGGAG GTGGACGAGC AGATGCTGGC CATCCAGAGC AAGAACAGCA
1081GCTACTTCGT GGAGTGGATC CCCAACAACG TGAAGGTGGC CGTGTGTGAC ATCCCGCCCC
1141GCGGCCTCAA GATGTCCTCC ACCTTCATCG GGAACAGCAC GGCCATCCAG GAGCTGTTCA
1201AGCGCATCTC CGAGCAGTTC ACGGCCATGT TCCGGCGCAA GGCCTTCCTG CACTGGTACA
1261CGGGCGAGGG CATGGACGAG ATGGAGTTCA CCGAGGCCGA GAGCAACATG AACGACCTGG
1321TGTCCGAGTA CCAGCAGTAC CAGGACGCCA CGGCCGAGGA AGAGGGCGAG ATGTACGAAG
1381ACGACGAGGA GGAGTCGGAG GCCCAGGGCC CCAAGTGCCC AACTTTCTTG TACAAAGTGG
1441TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1501CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1561ATTTCCTGAT TTATTTGACT TAATTACGCG TTAAGTCgac aatcaacctc tggattacaa
1621aatttgtgaa agatt

Download FASTA (ORF) (Full)