Construct: ORF TRCN0000470264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018975.3_s317c1
- Derived from:
- ccsbBroadEn_14950
- DNA Barcode:
- CTGCTGTTGCGGCCGTAACAACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT3 (10000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470264
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001370074.1 | 99% | 97.5% | (many diffs) |
| 2 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_005465.7 | 99% | 97.5% | (many diffs) |
| 3 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001206729.1 | 94.9% | 91.8% | (many diffs) |
| 4 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_181690.2 | 94.9% | 91.8% | (many diffs) |
| 5 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446000.1 | 88.5% | 86.4% | (many diffs) |
| 6 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_016999985.1 | 86.4% | 85% | (many diffs) |
| 7 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446892.1 | 59.5% | 55.6% | (many diffs) |
| 8 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_011544014.2 | 50% | 44.6% | (many diffs) |
| 9 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024447938.1 | 49.8% | 47.7% | (many diffs) |
| 10 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | NM_011785.3 | 93.1% | 97% | (many diffs) |
| 11 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496818.3 | 93.1% | 97% | (many diffs) |
| 12 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496819.3 | 93.1% | 97% | (many diffs) |
| 13 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_011238805.2 | 83.5% | 83.2% | (many diffs) |
| 14 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_017320418.1 | 80.9% | 84.5% | (many diffs) |
| 15 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496821.3 | 53.7% | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agatgttacc attgtgaaag aaggttgggt tcagaagagg ggagaatata 121 taaaaaactg gaggccaaga tacttccttt tgaagacaga tggctcattc ataggatata 181 aagagaaacc tcaagatgtg gatttacctt atcccctcaa caacttttca gtggcaaaat 241 gccagttaat gaaaacagaa cgaccaaagc caaacacatt tataatcaga tgtctccagt 301 ggactactgt tatagagaga acatttcatg tagatactcc agaggaaagg gaagaatgga 361 cagaagctat ccaggctgta gcagacagac tgcagaggca agaagaggag agaatgaatt 421 gtagtccaac ttcacaaatt gataatatag gagaggaaga gatggatgcc tctacaaccc 481 atcataaaag aaagacaatg aatgattttg actatttgaa actactaggt aaaggcactt 541 ttgggaaagt tattttggtt cgagagaagg caagtggaaa atactatgct atgaagattc 601 tgaagaaaga agtcattatt gcaaaggatg aagtggcaca cactctaact gaaagcagag 661 tattaaagaa cactagacat ccctttttaa catccttgaa ntatncntnc caganaaaag 721 acngtttgtg ttttgtgatg gaatatgtta nngggggcga gctgtttttc catttgtcga 781 gagagcgggt gttctctgag gaccgcacac gtttctatgg tgcagaaatt gtctctgcct 841 tggactatct acattcccgg naagatttgt ggtaccgtga tctcaagttg gagaatctaa 901 tgctggacaa agatggccac ataaaaatta cagattttgg actttgcaaa gaagggatca 961 cagatgcagc caccatgaag acattctgtg gcactccaga atatctggca ccagaggtgt 1021 tagaagataa tgactatggc cgagcagtag actggtgggg cctaggggtt gtcatgtatg 1081 aaatgatgtg tgggaggtta cctttctaca accaggacca tgagaaactt tttgaattaa 1141 tattaatgga agacattaaa tttcctcgaa cactctcttc agatgcaaaa tcattgcttt 1201 cagggctctt gataaaggat ccaaataaac gccttGGTGG AGGACCAGAT GATGCAAAAG 1261 AAATTATGAG ACACAGTTTC TTCTCTGGAG TAAACTGGCA AGATGTATAT GATAAAAAGC 1321 TTGTACCTCC TTTTAAACCT CAAGTAACAT CTGAGACAGA TACTAGATAT TTTGATGAAG 1381 AATTTACAGC TCAGACTATT ACAATAACAC CACCTGAAAA ATATGATGAG GATGGTATGG 1441 ACTGCATGGA CAATGAGAGG CGGCCGCATT TCCCTCAATT TTCCTACTCT GCAAGTGGAC 1501 GAGAATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGCTGTTG CGGCCGTAAC AACGTACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt