Construct: ORF TRCN0000470264

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018975.3_s317c1
Derived from:
ccsbBroadEn_14950
DNA Barcode:
CTGCTGTTGCGGCCGTAACAACGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKT3 (10000)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470264
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001370074.1 99% 97.5% (many diffs)
2 human 10000 AKT3 AKT serine/threonine kinase 3 NM_005465.7 99% 97.5% (many diffs)
3 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001206729.1 94.9% 91.8% (many diffs)
4 human 10000 AKT3 AKT serine/threonine kinase 3 NM_181690.2 94.9% 91.8% (many diffs)
5 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446000.1 88.5% 86.4% (many diffs)
6 human 10000 AKT3 AKT serine/threonine kinase 3 XM_016999985.1 86.4% 85% (many diffs)
7 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446892.1 59.5% 55.6% (many diffs)
8 human 10000 AKT3 AKT serine/threonine kinase 3 XM_011544014.2 50% 44.6% (many diffs)
9 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024447938.1 49.8% 47.7% (many diffs)
10 mouse 23797 Akt3 thymoma viral proto-oncogene 3 NM_011785.3 93.1% 97% (many diffs)
11 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496818.3 93.1% 97% (many diffs)
12 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496819.3 93.1% 97% (many diffs)
13 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_011238805.2 83.5% 83.2% (many diffs)
14 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_017320418.1 80.9% 84.5% (many diffs)
15 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496821.3 53.7% 51.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1506
ORF length:
1440
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag agatgttacc attgtgaaag aaggttgggt tcagaagagg ggagaatata
121taaaaaactg gaggccaaga tacttccttt tgaagacaga tggctcattc ataggatata
181aagagaaacc tcaagatgtg gatttacctt atcccctcaa caacttttca gtggcaaaat
241gccagttaat gaaaacagaa cgaccaaagc caaacacatt tataatcaga tgtctccagt
301ggactactgt tatagagaga acatttcatg tagatactcc agaggaaagg gaagaatgga
361cagaagctat ccaggctgta gcagacagac tgcagaggca agaagaggag agaatgaatt
421gtagtccaac ttcacaaatt gataatatag gagaggaaga gatggatgcc tctacaaccc
481atcataaaag aaagacaatg aatgattttg actatttgaa actactaggt aaaggcactt
541ttgggaaagt tattttggtt cgagagaagg caagtggaaa atactatgct atgaagattc
601tgaagaaaga agtcattatt gcaaaggatg aagtggcaca cactctaact gaaagcagag
661tattaaagaa cactagacat ccctttttaa catccttgaa ntatncntnc caganaaaag
721acngtttgtg ttttgtgatg gaatatgtta nngggggcga gctgtttttc catttgtcga
781gagagcgggt gttctctgag gaccgcacac gtttctatgg tgcagaaatt gtctctgcct
841tggactatct acattcccgg naagatttgt ggtaccgtga tctcaagttg gagaatctaa
901tgctggacaa agatggccac ataaaaatta cagattttgg actttgcaaa gaagggatca
961cagatgcagc caccatgaag acattctgtg gcactccaga atatctggca ccagaggtgt
1021tagaagataa tgactatggc cgagcagtag actggtgggg cctaggggtt gtcatgtatg
1081aaatgatgtg tgggaggtta cctttctaca accaggacca tgagaaactt tttgaattaa
1141tattaatgga agacattaaa tttcctcgaa cactctcttc agatgcaaaa tcattgcttt
1201cagggctctt gataaaggat ccaaataaac gccttGGTGG AGGACCAGAT GATGCAAAAG
1261AAATTATGAG ACACAGTTTC TTCTCTGGAG TAAACTGGCA AGATGTATAT GATAAAAAGC
1321TTGTACCTCC TTTTAAACCT CAAGTAACAT CTGAGACAGA TACTAGATAT TTTGATGAAG
1381AATTTACAGC TCAGACTATT ACAATAACAC CACCTGAAAA ATATGATGAG GATGGTATGG
1441ACTGCATGGA CAATGAGAGG CGGCCGCATT TCCCTCAATT TTCCTACTCT GCAAGTGGAC
1501GAGAATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1561TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1621CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGCTGTTG CGGCCGTAAC AACGTACGCG
1681TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)