Construct: ORF TRCN0000471778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015529.1_s317c1
- Derived from:
- ccsbBroadEn_12038
- DNA Barcode:
- CAAGTTTGACTAGCTTGAAGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAG3L1 (54441)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471778
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 442582 | STAG3L2 | stromal antigen 3-like 2 (p... | NR_040584.2 | 12.5% | 1_738del;771C>T;976_1874del | |
| 2 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956445.1 | 11.6% | (many diffs) | |
| 3 | human | 54441 | STAG3L1 | stromal antigen 3-like 1 (p... | NR_040583.2 | 11% | 1_994del;1232_2138del | |
| 4 | human | 442578 | STAG3L3 | stromal antigen 3-like 3 (p... | NR_040582.2 | 10.9% | 1_1013del;1251_2167del | |
| 5 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744735.1 | 10.9% | 1_646del;884_2172del | |
| 6 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744750.1 | 10.5% | 1_725del;963_2251del | |
| 7 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744736.1 | 10.4% | 1_734del;972_2260del | |
| 8 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744739.2 | 9.8% | 1_878del;1116_2404del | |
| 9 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744737.2 | 9.6% | 1_918del;1156_2444del | |
| 10 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282718.2 | 6.2% | 5.1% | (many diffs) |
| 11 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011687.1 | 6.1% | 5.1% | (many diffs) |
| 12 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282716.1 | 5.9% | 4.9% | (many diffs) |
| 13 | human | 10734 | STAG3 | stromal antigen 3 | NM_012447.4 | 5.9% | 4.9% | (many diffs) |
| 14 | human | 10734 | STAG3 | stromal antigen 3 | NM_001282717.1 | 5.9% | 4.9% | (many diffs) |
| 15 | human | 10734 | STAG3 | stromal antigen 3 | XM_011515742.1 | 5.9% | 4.9% | (many diffs) |
| 16 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011686.2 | 5.8% | 4.8% | (many diffs) |
| 17 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011683.2 | 5.8% | 4.8% | (many diffs) |
| 18 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011684.1 | 5.8% | 4.8% | (many diffs) |
| 19 | human | 10734 | STAG3 | stromal antigen 3 | XM_017011685.1 | 5.8% | 4.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 204
- ORF end:
- 441
- ORF length:
- 237
- Sequence:
-
1 GGAGTTTCCC CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA 61 ATTCTCCTTG GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC 121 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA 181 CAAGTTTGTA CAAAAAAGTT GGCATGGGTG GAAGAGAGCA ACGCCAGAGC CCAGGTGCCC 241 AGAGGACTTT CTTCCAGCTT CTGCTGTCCT TCTTTGTGGA GAGCAAGCTC CACGACCACG 301 CTGCTTACTT AGTAGACAAC CTGTGGGACT GTGCAGGGAC TCAGCTGAAG GACTGGGAGG 361 GTCTGACAAG CCTGCTGCTG GAGAAGGACC AGAGCACGTG CCACATGGAG CCAGGGCCAG 421 GGACCTTCCA CCTCCTAGGG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAG TTTGACTAGC 601 TTGAAGTGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt