Construct: ORF TRCN0000471889

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018481.1_s317c1
Derived from:
ccsbBroadEn_13754
DNA Barcode:
GAGCTCTCCAGTGTGATCTGCACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIR2DS2 (100132285)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471889
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291695.1 99.8% 99.7% 11_12delTGinsCT
2 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_012312.4 87.8% 84.3% (many diffs)
3 human 100132285 KIR2DS2 killer cell immunoglobulin ... XM_017030275.2 87.8% 84.3% (many diffs)
4 human 3803 KIR2DL2 killer cell immunoglobulin ... NM_014219.2 87.6% 85.8% (many diffs)
5 human 3804 KIR2DL3 killer cell immunoglobulin ... NM_015868.3 86.2% 83.6% (many diffs)
6 human 3802 KIR2DL1 killer cell immunoglobulin ... NM_014218.3 85.4% 81.9% (many diffs)
7 human 112267881 LOC112267881 killer cell immunoglobulin-... NM_001368251.1 85.3% 81.3% (many diffs)
8 human 3806 KIR2DS1 killer cell immunoglobulin ... NM_014512.1 84.9% 79.4% (many diffs)
9 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_011547998.2 84.7% 79.1% (many diffs)
10 human 3808 KIR2DS3 killer cell immunoglobulin ... NM_012313.1 84.7% 77.1% (many diffs)
11 human 102725023 LOC102725023 killer cell immunoglobulin-... NM_001360171.1 84.2% 77.3% (many diffs)
12 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_012314.6 83.9% 77.1% (many diffs)
13 human 3810 KIR2DS5 killer cell immunoglobulin ... NM_014513.2 83.7% 77.1% (many diffs)
14 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291701.1 82.8% 79.4% (many diffs)
15 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282170.1 77.8% 67.4% (many diffs)
16 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452542.1 76.5% 66% (many diffs)
17 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282171.1 75.1% 65.4% (many diffs)
18 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026782.1 71.2% 67.7% (many diffs)
19 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026783.1 70.5% 64.3% (many diffs)
20 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452543.1 69.5% 64.3% (many diffs)
21 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452544.1 69.5% 64.3% (many diffs)
22 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452545.1 69.5% 64.3% (many diffs)
23 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_006737.4 68.2% 62.8% (many diffs)
24 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281971.2 66.6% 47.9% (many diffs)
25 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_001322168.1 66% 60.2% (many diffs)
26 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_013289.2 66% 60.2% (many diffs)
27 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_001242867.2 64.7% 59.5% (many diffs)
28 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_017030286.1 64.7% 60.6% (many diffs)
29 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_011526939.2 64.5% 60.3% (many diffs)
30 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281972.2 61.6% 45.5% (many diffs)
31 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_011526940.3 61.5% 56.7% (many diffs)
32 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001083539.2 61.1% 52.8% (many diffs)
33 human 112268355 LOC112268355 killer cell immunoglobulin-... NM_001368254.1 61.1% 52.8% (many diffs)
34 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291696.1 58.5% 55.3% (many diffs)
35 human 3811 KIR3DL1 killer cell immunoglobulin ... XM_017030274.1 58% 44.2% (many diffs)
36 human 115653 KIR3DL3 killer cell immunoglobulin ... NM_153443.4 57.4% 50.7% (many diffs)
37 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_017026784.2 51.8% 47.6% (many diffs)
38 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291700.1 25.9% 26.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1083
ORF length:
1014
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgctcact gtcgtcagca tggcgtgtgt tgggttcttc ttgctgcagg
121gggcctggcc acatgaggga gtccacagaa aaccttccct cctggcccac ccaggtcccc
181tggtgaaatc agaagagaca gtcatcctgc aatgttggtc agatgtcagg tttgagcact
241tccttctgca cagagagggg aagtataagg acactttgca cctcattgga gagcaccatg
301atggggtctc caaggccaac ttctccatcg gtcccatgat gcaagacctt gcagggacct
361acagatgcta cggttctgtt actcactccc cctatcagtt gtcagctccc agtgaccctc
421tggacatcgt catcacaggt ctatatgaga aaccttctct ctcagcccag ccgggcccca
481cggttttggc aggagagagc gtgaccttgt cctgcagctc ccggagctcc tatgacatgt
541accatctatc cagggagggg gaggcccatg aacgtaggtt ctctgcaggg cccaaggtca
601acggaacatt ccaggccgac tttcctctgg gccctgccac ccacggagga acctacagat
661gcttcggctc tttccgtgac tctccctatg agtggtcaaa ctcgagtgac ccactgcttg
721tttctgtcac aggaaaccct tcaaatagtt ggccttcacc cactgaacca agctccaaaa
781ccggtaaccc cagacacctg catgttctga ttgggacCTC AGTGGTCAAA ATCCCTTTCA
841CCATCCTCCT CTTCTTTCTC CTTCATCGCT GGTGCTCCAA CAAAAAAAAT GCTGCTGTAA
901TGGACCAAGA GCCTGCAGGG AACAGAACAG TGAACAGCGA GAGAGAAATC ACTCGCCCTT
961CTGAGAGGCC CAAGACACCC CCAACAGATA CCAGCATGTA CATAGAACTT CCAAATGCTG
1021AGCCCAGATC CAAAGTTGTC TTCTGTCCAC GAGCACCACA GTCAGGCCTT GAGGGGATCT
1081TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1141TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1201GGCTTTATAT ATCTTGTGGA AAGGACGAGA GCTCTCCAGT GTGATCTGCA CGACGCGTTA
1261AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)