Transcript: Human XM_017030275.2

PREDICTED: Homo sapiens killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 2 (KIR2DS2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KIR2DS2 (100132285)
Length:
1692
CDS:
153..1067

Additional Resources:

NCBI RefSeq record:
XM_017030275.2
NBCI Gene record:
KIR2DS2 (100132285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017030275.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243133 GGAGGTGTCATACGCATAATT pLKO_005 1049 CDS 100% 15.000 7.500 Y KIR3DS1 n/a
2 TRCN0000057029 CATCGTCATCACAGGTCTATA pLKO.1 509 CDS 100% 13.200 6.600 Y KIR2DS2 n/a
3 TRCN0000435229 GAGGTGTCATACGCATAATTG pLKO_005 1050 CDS 100% 13.200 6.600 Y KIR2DS4 n/a
4 TRCN0000428282 GCCTCTCTCTTGCTTACAAAT pLKO_005 1356 3UTR 100% 13.200 6.600 Y KIR2DL1 n/a
5 TRCN0000057032 GTCACAGGAAACCCTTCAAAT pLKO.1 810 CDS 100% 13.200 6.600 Y KIR2DS2 n/a
6 TRCN0000421852 TCCTTTGCTTAGCCCACAATT pLKO_005 1415 3UTR 100% 13.200 6.600 Y KIR2DS5 n/a
7 TRCN0000418745 AGGCATCAGTCTTCATCTTAG pLKO_005 1300 3UTR 100% 10.800 5.400 Y KIR2DS5 n/a
8 TRCN0000243132 CTATGACATGTACCATCTATC pLKO_005 614 CDS 100% 10.800 5.400 Y KIR3DS1 n/a
9 TRCN0000057030 CCTATGACATGTACCATCTAT pLKO.1 613 CDS 100% 5.625 2.813 Y KIR2DS2 n/a
10 TRCN0000057031 CCTGCAATGTTGGTCAGATGT pLKO.1 290 CDS 100% 4.950 2.475 Y KIR2DS2 n/a
11 TRCN0000056989 CTACAGATGCTTCGGCTCTTT pLKO.1 737 CDS 100% 4.950 2.475 Y KIR2DS1 n/a
12 TRCN0000056930 CTCCTCTTCTTTCTCCTTCAT pLKO.1 930 CDS 100% 4.950 2.475 Y KIR2DS5 n/a
13 TRCN0000056929 GCTTGTTTCTGTCACAGGAAA pLKO.1 800 CDS 100% 4.950 2.475 Y KIR2DS5 n/a
14 TRCN0000063691 TGCAGGGAACAGAACAGTGAA pLKO.1 998 CDS 100% 4.950 2.475 Y KIR2DL3 n/a
15 TRCN0000061717 TCAGGAGGTGTCATACGCATA pLKO.1 1046 CDS 100% 4.050 2.025 Y KIR2DS3 n/a
16 TRCN0000056931 CAGGTCTATATGAGAAACCTT pLKO.1 520 CDS 100% 3.000 1.500 Y KIR2DS5 n/a
17 TRCN0000056932 CCCTGGTGAAATCAGAAGAGA pLKO.1 262 CDS 100% 3.000 1.500 Y KIR2DS5 n/a
18 TRCN0000057028 GTCAGGTTTGAGCACTTCCTT pLKO.1 309 CDS 100% 3.000 1.500 Y KIR2DS2 n/a
19 TRCN0000056990 GATTCTGATGAACAAGACCAT pLKO.1 1026 CDS 100% 2.640 1.320 Y KIR2DS1 n/a
20 TRCN0000056758 CCTGCAATGTTGGTCAGATAT pLKO.1 290 CDS 100% 13.200 6.600 Y KIR3DL1 n/a
21 TRCN0000063023 CCTCCTCTTCTTTCTCCTTTA pLKO.1 929 CDS 100% 10.800 5.400 Y KIR3DL2 n/a
22 TRCN0000417192 TTGGGACCTCAGTGGTCAAAC pLKO_005 895 CDS 100% 10.800 5.400 Y KIR2DS5 n/a
23 TRCN0000061458 CCACTGCTTGTTTCTGTCATA pLKO.1 795 CDS 100% 4.950 2.475 Y KIR2DL2 n/a
24 TRCN0000056825 GCAATGTTGGTCAGATGTCAT pLKO.1 293 CDS 100% 4.950 2.475 Y KIR2DL1 n/a
25 TRCN0000149432 GCAATGTTGGTCAGATGTCAT pLKO.1 293 CDS 100% 4.950 2.475 Y KIR3DP1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017030275.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000479236 CTGTCAGATCCGTACCTGTCATTG pLX_317 52% 95.8% 79.9% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14687 pDONR223 73.4% 95.3% 78.9% None (many diffs) n/a
3 ccsbBroad304_14687 pLX_304 0% 95.3% 78.9% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000474077 AGACCGCGTCCAACGTCATACTTT pLX_317 34.7% 95.3% 78.9% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_13888 pDONR223 100% 95.7% 3% None (many diffs) n/a
6 ccsbBroad304_13888 pLX_304 0% 95.7% 3% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000479468 TCAGTAGCAAGTTATTCAATCTAG pLX_317 32.5% 95.7% 3% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_13754 pDONR223 100% 87.8% 84.3% None (many diffs) n/a
9 ccsbBroad304_13754 pLX_304 0% 87.8% 84.3% V5 (many diffs) n/a
10 TRCN0000471889 GAGCTCTCCAGTGTGATCTGCACG pLX_317 26.6% 87.8% 84.3% V5 (many diffs) n/a
11 ccsbBroadEn_06487 pDONR223 100% 86.9% 84.7% None (many diffs) n/a
12 ccsbBroad304_06487 pLX_304 0% 86.9% 84.7% V5 (many diffs) n/a
13 TRCN0000474884 GAAATACACGTCGTTCCGCTTCCC pLX_317 47.5% 86.9% 84.7% V5 (many diffs) n/a
14 ccsbBroadEn_10936 pDONR223 100% 76.6% 73.6% None (many diffs) n/a
15 ccsbBroad304_10936 pLX_304 0% 76.6% 73.6% V5 (many diffs) n/a
16 TRCN0000475707 TATTACGCGGTACACTTGCGGTTC pLX_317 26.1% 76.6% 73.6% V5 (many diffs) n/a
17 ccsbBroadEn_13889 pDONR223 100% 66.5% 38% None (many diffs) n/a
18 ccsbBroadEn_00908 pDONR223 100% 66.5% 57.5% None (many diffs) n/a
19 ccsbBroad304_00908 pLX_304 0% 66.5% 57.5% V5 (many diffs) n/a
20 TRCN0000472352 TATTAACAAGCCGTGATAGGACCA pLX_317 26.7% 66.5% 57.5% V5 (many diffs) n/a
21 ccsbBroadEn_06489 pDONR223 100% 63% 57.6% None (many diffs) n/a
22 ccsbBroad304_06489 pLX_304 0% 63% 57.6% V5 (many diffs) n/a
23 TRCN0000469534 TAGGTTCAGTACAATACTGACCCA pLX_317 32.5% 63% 57.6% V5 (many diffs) n/a
24 ccsbBroadEn_00907 pDONR223 100% 61.2% 56.4% None (many diffs) n/a
25 ccsbBroad304_00907 pLX_304 0% 61.2% 56.4% V5 (many diffs) n/a
26 TRCN0000492063 GACTAACCGAGACGTTGGGATCTG pLX_317 9.5% 61.2% 56.4% V5 (many diffs) n/a
27 ccsbBroadEn_09418 pDONR223 100% 59.5% 52.9% None (many diffs) n/a
28 ccsbBroad304_09418 pLX_304 0% 59.5% 52.9% V5 (many diffs) n/a
Download CSV