Transcript: Human NM_013289.2

Homo sapiens killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1 (KIR3DL1), transcript variant 1 (reference allele), mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
KIR3DL1 (3811)
Length:
1986
CDS:
64..1398

Additional Resources:

NCBI RefSeq record:
NM_013289.2
NBCI Gene record:
KIR3DL1 (3811)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_013289.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000056759 CCCTACAGATACCATCTTGTA pLKO.1 1326 CDS 100% 4.950 6.930 N KIR3DL1 n/a
2 TRCN0000418077 GCCAACAGCGAGGACTCTGAT pLKO_005 1210 CDS 100% 1.650 1.155 N KIR3DL1 n/a
3 TRCN0000425799 GCTTACAAATGTCTAGGTCCC pLKO_005 1560 3UTR 100% 1.200 0.720 N KIR3DL1 n/a
4 TRCN0000056758 CCTGCAATGTTGGTCAGATAT pLKO.1 486 CDS 100% 13.200 6.600 Y KIR3DL1 n/a
5 TRCN0000428282 GCCTCTCTCTTGCTTACAAAT pLKO_005 1549 3UTR 100% 13.200 6.600 Y KIR2DL1 n/a
6 TRCN0000056760 CCACAGAACCAAGCTCCAAAT pLKO.1 1040 CDS 100% 10.800 5.400 Y KIR3DL1 n/a
7 TRCN0000243132 CTATGACATGTACCATCTATC pLKO_005 810 CDS 100% 10.800 5.400 Y KIR3DS1 n/a
8 TRCN0000243131 TCGGTGTCACTATCGTCATAG pLKO_005 204 CDS 100% 10.800 5.400 Y KIR3DS1 n/a
9 TRCN0000057030 CCTATGACATGTACCATCTAT pLKO.1 809 CDS 100% 5.625 2.813 Y KIR2DS2 n/a
10 TRCN0000056762 GCGCAAGGTCAACAGAACATT pLKO.1 870 CDS 100% 5.625 2.813 Y KIR3DL1 n/a
11 TRCN0000056989 CTACAGATGCTTCGGCTCTTT pLKO.1 933 CDS 100% 4.950 2.475 Y KIR2DS1 n/a
12 TRCN0000056930 CTCCTCTTCTTTCTCCTTCAT pLKO.1 1126 CDS 100% 4.950 2.475 Y KIR2DS5 n/a
13 TRCN0000056761 GCTATACAAAGAAGACAGAAT pLKO.1 240 CDS 100% 4.950 2.475 Y KIR3DL1 n/a
14 TRCN0000056929 GCTTGTTTCTGTCACAGGAAA pLKO.1 996 CDS 100% 4.950 2.475 Y KIR2DS5 n/a
15 TRCN0000057032 GTCACAGGAAACCCTTCAAAT pLKO.1 1006 CDS 100% 13.200 6.600 Y KIR2DS2 n/a
16 TRCN0000063023 CCTCCTCTTCTTTCTCCTTTA pLKO.1 1125 CDS 100% 10.800 5.400 Y KIR3DL2 n/a
17 TRCN0000061458 CCACTGCTTGTTTCTGTCATA pLKO.1 991 CDS 100% 4.950 2.475 Y KIR2DL2 n/a
18 TRCN0000057031 CCTGCAATGTTGGTCAGATGT pLKO.1 486 CDS 100% 4.950 2.475 Y KIR2DS2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013289.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06489 pDONR223 100% 99.6% 99.3% None (many diffs) n/a
2 ccsbBroad304_06489 pLX_304 0% 99.6% 99.3% V5 (many diffs) n/a
3 TRCN0000469534 TAGGTTCAGTACAATACTGACCCA pLX_317 32.5% 99.6% 99.3% V5 (many diffs) n/a
4 ccsbBroadEn_00907 pDONR223 100% 90.9% 83.5% None (many diffs) n/a
5 ccsbBroad304_00907 pLX_304 0% 90.9% 83.5% V5 (many diffs) n/a
6 TRCN0000492063 GACTAACCGAGACGTTGGGATCTG pLX_317 9.5% 90.9% 83.5% V5 (many diffs) n/a
7 ccsbBroadEn_00908 pDONR223 100% 84.4% 79.1% None (many diffs) n/a
8 ccsbBroad304_00908 pLX_304 0% 84.4% 79.1% V5 (many diffs) n/a
9 TRCN0000472352 TATTAACAAGCCGTGATAGGACCA pLX_317 26.7% 84.4% 79.1% V5 (many diffs) n/a
10 ccsbBroadEn_13889 pDONR223 100% 84.3% 62.3% None (many diffs) n/a
11 ccsbBroadEn_09418 pDONR223 100% 80.2% 70% None (many diffs) n/a
12 ccsbBroad304_09418 pLX_304 0% 80.2% 70% V5 (many diffs) n/a
13 ccsbBroadEn_06487 pDONR223 100% 70.8% 64.4% None (many diffs) n/a
14 ccsbBroad304_06487 pLX_304 0% 70.8% 64.4% V5 (many diffs) n/a
15 TRCN0000474884 GAAATACACGTCGTTCCGCTTCCC pLX_317 47.5% 70.8% 64.4% V5 (many diffs) n/a
16 ccsbBroadEn_10936 pDONR223 100% 68.2% 62.3% None (many diffs) n/a
17 ccsbBroad304_10936 pLX_304 0% 68.2% 62.3% V5 (many diffs) n/a
18 TRCN0000475707 TATTACGCGGTACACTTGCGGTTC pLX_317 26.1% 68.2% 62.3% V5 (many diffs) n/a
19 ccsbBroadEn_13754 pDONR223 100% 66% 60.2% None (many diffs) n/a
20 ccsbBroad304_13754 pLX_304 0% 66% 60.2% V5 (many diffs) n/a
21 TRCN0000471889 GAGCTCTCCAGTGTGATCTGCACG pLX_317 26.6% 66% 60.2% V5 (many diffs) n/a
22 ccsbBroadEn_06488 pDONR223 100% 62.1% 52% None (many diffs) n/a
23 ccsbBroad304_06488 pLX_304 0% 62.1% 52% V5 (many diffs) n/a
24 TRCN0000480702 ACTCACGGACTGCAGTACACGCGC pLX_317 26.5% 62.1% 52% V5 (many diffs) n/a
25 ccsbBroadEn_13888 pDONR223 100% 61.9% 2.1% None (many diffs) n/a
26 ccsbBroad304_13888 pLX_304 0% 61.9% 2.1% V5 (not translated due to prior stop codon) (many diffs) n/a
27 TRCN0000479468 TCAGTAGCAAGTTATTCAATCTAG pLX_317 32.5% 61.9% 2.1% V5 (not translated due to prior stop codon) (many diffs) n/a
28 TRCN0000479236 CTGTCAGATCCGTACCTGTCATTG pLX_317 52% 61.7% 48.4% V5 (not translated due to prior stop codon) (many diffs) n/a
29 TRCN0000474077 AGACCGCGTCCAACGTCATACTTT pLX_317 34.7% 61.7% 48.4% V5 (not translated due to prior stop codon) (many diffs) n/a
30 ccsbBroadEn_14687 pDONR223 73.4% 61.7% 48.1% None (many diffs) n/a
31 ccsbBroad304_14687 pLX_304 0% 61.7% 48.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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