Construct: ORF TRCN0000472194
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007765.1_s317c1
- Derived from:
- ccsbBroadEn_11765
- DNA Barcode:
- AAATCCCGAAGCATGGTTGGCACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D22A (25771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472194
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | NM_001284303.1 | 100% | 100% | |
| 2 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028745.2 | 95.8% | 95.8% | 63_119del |
| 3 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | NM_001284304.1 | 85.2% | 84.5% | (many diffs) |
| 4 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | NM_014346.5 | 84.9% | 84.9% | 63_119del;460_636del |
| 5 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | NM_001284305.1 | 82.5% | 82.5% | 0_1ins84;319_495del |
| 6 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028744.1 | 82.5% | 82.5% | 0_1ins84;319_495del |
| 7 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028742.2 | 79.9% | 79.9% | 63_119del;460_636del;1126_1221del |
| 8 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_024452198.1 | 79.2% | 76.9% | (many diffs) |
| 9 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028743.2 | 77.9% | 72.2% | (many diffs) |
| 10 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_011530099.2 | 77.1% | 77.5% | (many diffs) |
| 11 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_011530100.3 | 62.3% | 62.2% | 63_119del;460_636del;1202_1203delTCins350 |
| 12 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028746.2 | 59.5% | 58% | (many diffs) |
| 13 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_011530101.3 | 57.5% | 57.4% | 63_119del;460_636del;1128_1128delAins424 |
| 14 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_011530102.3 | 54.9% | 50% | (many diffs) |
| 15 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_005261496.2 | 54.3% | 51.4% | (many diffs) |
| 16 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XM_017028747.2 | 53% | 51.6% | (many diffs) |
| 17 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_002958682.1 | 34.9% | (many diffs) | |
| 18 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_001755206.2 | 34.8% | (many diffs) | |
| 19 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_001755203.2 | 33.6% | (many diffs) | |
| 20 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_001755202.2 | 33.2% | (many diffs) | |
| 21 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_001755204.1 | 33.2% | (many diffs) | |
| 22 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | XR_001755205.1 | 32.1% | (many diffs) | |
| 23 | human | 25771 | TBC1D22A | TBC1 domain family member 22A | NR_104292.1 | 29% | (many diffs) | |
| 24 | mouse | 223754 | Tbc1d22a | TBC1 domain family, member 22a | NM_145476.2 | 73.4% | 76.9% | (many diffs) |
| 25 | mouse | 223754 | Tbc1d22a | TBC1 domain family, member 22a | XM_006520833.2 | 68% | 71.3% | (many diffs) |
| 26 | mouse | 223754 | Tbc1d22a | TBC1 domain family, member 22a | XM_017316576.1 | 57.6% | 61.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcgacggg gccaggaagc aattctggaa gcgcagcaac agcaagctcc 121 cgggcagttt gctcaggtcc acggccaaga tgccgaccac accagtgaag gccaagaggg 181 tcagcacctt ccaggagttt gagagcaata ccagcgatgc ctgggacgct ggggaggacg 241 acgatgagct cctggccatg gcggcggaga gcctgaactc cgaggtggtc atggagacgg 301 ccaaccgtgt gctgcgtaac cacagccagc ggcaggggcg gcccacgctg caggaggggc 361 cagggcttca gcagaagccc aggcccgagg cagagccgcc ctcacccccc agcggcgacc 421 tccggctggt gaagtcggtc agtgagagcc acacgtcctg tcctgcagag gaattacgga 481 ggttgagctg gtccggaatc cctaagccag tgcgtccaat gacgtggaag ctcctctcag 541 gttaccttcc cgccaatgta gaccggagac cagccactct ccagagaaaa caaaaagaat 601 attttgcatt tattgagcac tattacgatt ctaggaacga cgaagttcac caggacacat 661 acaggcagat ccacatagac atccctcgca tgagccctga agcgttgatc ctgcagccca 721 aggtgacgga gatttttgaa aggatcttgt tcatatgggc gatccgccac ccagccagtg 781 gatacgttca gggtataaat gatctcgtca ctcctttctt tgtggtcttc atttgtgaat 841 acatagaggc agaggaggtg gacacggtgg acgtcTCCGG CGTGCCCGCA GAGGTGCTGT 901 GCAACATCGA GGCCGACACC TACTGGTGCA TGAGCAAGCT GCTGGATGGC ATTCAGGACA 961 ACTACACCTT TGCCCAACCT GGGATTCAAA TGAAAGTGAA AATGTTAGAA GAACTCGTGA 1021 GCCGGATTGA TGAGCAAGTG CACCGGCACC TGGACCAACA CGAAGTGAGA TACCTGCAGT 1081 TTGCCTTCCG CTGGATGAAC AACCTGCTGA TGAGGGAGGT GCCCCTGCGT TGTACCATCC 1141 GCCTGTGGGA CACCTACCAG TCTGAACCGG ACGGCTTTTC TCATTTCCAC TTGTACGTGT 1201 GCGCTGCTTT TCTCGTGAGA TGGAGGAAGG AAATACTAGA AGAAAAAGAT TTTCAAGAGC 1261 TGCTGCTCTT CCTCCAGAAC CTGCCCACAG CCCACTGGGA TGATGAGGAC ATCAGCCTGT 1321 TGCTGGCCGA GGCCTACCGC CTCAAGTTTG CTTTTGCCGA CGCCCCCAAT CACTACAAGA 1381 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAAA ATCCCGAAGC ATGGTTGGCA CCACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt