Construct: ORF TRCN0000474077
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006828.1_s317c1
- Derived from:
- ccsbBroadEn_14687
- DNA Barcode:
- AGACCGCGTCCAACGTCATACTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KIR2DS4 (3809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474077
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_012314.6 | 98% | 83.5% | (many diffs) |
| 2 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | NM_014512.1 | 97% | 80.9% | (many diffs) |
| 3 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_011547998.2 | 97% | 80.9% | (many diffs) |
| 4 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_012312.4 | 95.3% | 78.9% | (many diffs) |
| 5 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | XM_017030275.2 | 95.3% | 78.9% | (many diffs) |
| 6 | human | 3810 | KIR2DS5 | killer cell immunoglobulin ... | NM_014513.2 | 94.5% | 77.3% | (many diffs) |
| 7 | human | 102725023 | LOC102725023 | killer cell immunoglobulin-... | NM_001360171.1 | 94.1% | 76.6% | (many diffs) |
| 8 | human | 3808 | KIR2DS3 | killer cell immunoglobulin ... | NM_012313.1 | 94.1% | 76.6% | (many diffs) |
| 9 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291701.1 | 89.9% | 73.6% | (many diffs) |
| 10 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001282170.1 | 85.2% | 74.9% | (many diffs) |
| 11 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291695.1 | 83.9% | 71% | (many diffs) |
| 12 | human | 3804 | KIR2DL3 | killer cell immunoglobulin ... | NM_015868.3 | 83.9% | 69.2% | (many diffs) |
| 13 | human | 112267881 | LOC112267881 | killer cell immunoglobulin-... | NM_001368251.1 | 82.3% | 69% | (many diffs) |
| 14 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001282171.1 | 82.2% | 72.8% | (many diffs) |
| 15 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452542.1 | 82% | 77% | (many diffs) |
| 16 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | NM_014218.3 | 81.8% | 67.9% | (many diffs) |
| 17 | human | 3803 | KIR2DL2 | killer cell immunoglobulin ... | NM_014219.2 | 81.3% | 67.6% | (many diffs) |
| 18 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452543.1 | 80.3% | 65.7% | (many diffs) |
| 19 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452544.1 | 80.3% | 65.7% | (many diffs) |
| 20 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452545.1 | 80.3% | 65.7% | (many diffs) |
| 21 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_001281971.2 | 76.8% | 57.3% | (many diffs) |
| 22 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_017026783.1 | 76.1% | 76.6% | (many diffs) |
| 23 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_017030286.1 | 72.5% | 73.9% | (many diffs) |
| 24 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_011526939.2 | 71.8% | 72.4% | (many diffs) |
| 25 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_001281972.2 | 71.3% | 60% | (many diffs) |
| 26 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_017026782.1 | 67.4% | 54.7% | (many diffs) |
| 27 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001083539.2 | 65.1% | 57% | (many diffs) |
| 28 | human | 112268355 | LOC112268355 | killer cell immunoglobulin-... | NM_001368254.1 | 65.1% | 57% | (many diffs) |
| 29 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291696.1 | 64.3% | 49.6% | (many diffs) |
| 30 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | NM_001322168.1 | 61.9% | 48.8% | (many diffs) |
| 31 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | NM_013289.2 | 61.7% | 48.4% | (many diffs) |
| 32 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | NM_006737.4 | 60.6% | 48.1% | (many diffs) |
| 33 | human | 115653 | KIR3DL3 | killer cell immunoglobulin ... | NM_153443.4 | 59.2% | 44% | (many diffs) |
| 34 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | XM_017030274.1 | 57.3% | 49.1% | (many diffs) |
| 35 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | NM_001242867.2 | 57.2% | 45% | (many diffs) |
| 36 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | XM_017026784.2 | 55.9% | 54.3% | (many diffs) |
| 37 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | XM_011526940.3 | 54.2% | 49.5% | (many diffs) |
| 38 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291700.1 | 32% | 20.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 897
- ORF length:
- 828
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgctcatg gtcatcagca tggcgtgtgt tgggttcttc ttgctgcagg 121 gggcctggcc acaggaggga gtccacagaa aaccttcctt cctggccctc ccaggtcacc 181 tggtgaaatc agaagagaca gtcatcctgc aatgttggtc agatgtcatg tttgaacact 241 tccttctgca cagagagggg atgtttaacg acactttgca cctcattgga gagcaccatg 301 atggggtttc caaggccaac ttctccattg gtcccatgat gcctgtcctt gcaggaacct 361 acagatgcta cggttctgtt cctcactccc cctatcagtt gtcagctccc agtgaccctc 421 tggacatcgt gatcataggt ctatatgaga aaccttctct ctcagcccag ccgggcccca 481 cggttctggc aggagagaat gtgaccttgt cctgcagctc ccggagctcc tatgacatgt 541 accatctatc cagggaaggg gaggcccatg aacgtaggct ccctgcagtg cgcagcatca 601 acggaacatt ccaggccaac tttcctctgg gccctgccac ccatggaggg acctacagat 661 gcttcGGCTC TTTCCGTGAC TCTCCATACG AGTGGTCAAA GTCAAGTGAC CCACTGCTTG 721 TTTCTGTCAC AGGAAACCCT TCAAATAGTT GGCCTTCACC CACTGAACCA AGCTCCGAAA 781 CCGGTAACCC CAGACACCTA CATGTTCTGA TTGGGACCTC AGTGGTCAAA ATCCCTTTCA 841 CCATCCTCCT CTCTTTCTCC TTCATCGCTG GTGCTCCGAC AAAAAAAATG CTGCTGTAAT 901 GGACCAAGAG CCTGCAGGGA ACAGAACAGT GAACAGCGAG GATTCTGATG AACAAGACCA 961 TCAGGAGGTG TCATACGCAT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1021 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1081 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGACC GCGTCCAACG 1141 TCATACTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt