Construct: ORF TRCN0000474228

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005596.1_s317c1
Derived from:
ccsbBroadEn_08597
DNA Barcode:
GGCGATTCGTACCCTCACTGGCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGAP15 (55843)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474228
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55843 ARHGAP15 Rho GTPase activating prote... NM_018460.4 99.9% 99.7% 1226T>C
2 human 55843 ARHGAP15 Rho GTPase activating prote... XM_011511479.2 99.9% 99.7% 1226T>C
3 human 55843 ARHGAP15 Rho GTPase activating prote... XM_017004499.2 99.9% 99.7% 1226T>C
4 human 55843 ARHGAP15 Rho GTPase activating prote... XM_011511481.2 90.3% 87.6% (many diffs)
5 human 55843 ARHGAP15 Rho GTPase activating prote... XM_017004500.2 90.1% 88.8% (many diffs)
6 human 55843 ARHGAP15 Rho GTPase activating prote... XM_011511482.2 87.4% 86.9% (many diffs)
7 human 55843 ARHGAP15 Rho GTPase activating prote... XM_011511483.1 77.8% 70.2% (many diffs)
8 human 55843 ARHGAP15 Rho GTPase activating prote... XM_011511484.1 71.6% 70% (many diffs)
9 human 55843 ARHGAP15 Rho GTPase activating prote... XM_017004501.1 69.2% 66.1% (many diffs)
10 human 55843 ARHGAP15 Rho GTPase activating prote... XR_001738850.1 52.1% (many diffs)
11 human 55843 ARHGAP15 Rho GTPase activating prote... XM_024453000.1 50.6% 50.5% 0_1ins702;524T>C
12 human 55843 ARHGAP15 Rho GTPase activating prote... XM_024453001.1 50.6% 50.5% 0_1ins702;524T>C
13 mouse 76117 Arhgap15 Rho GTPase activating prote... NM_153820.3 86.6% 84.8% (many diffs)
14 mouse 76117 Arhgap15 Rho GTPase activating prote... XM_006498399.3 83.8% 82.9% (many diffs)
15 mouse 76117 Arhgap15 Rho GTPase activating prote... XM_006498398.3 82.6% 80.7% (many diffs)
16 mouse 76117 Arhgap15 Rho GTPase activating prote... XM_006498400.3 79.9% 75.6% (many diffs)
17 mouse 76117 Arhgap15 Rho GTPase activating prote... XM_006498397.3 78.9% 73.4% (many diffs)
18 mouse 76117 Arhgap15 Rho GTPase activating prote... NM_001301831.1 78.6% 75.7% (many diffs)
19 mouse 76117 Arhgap15 Rho GTPase activating prote... XM_006498402.3 72.5% 70.4% (many diffs)
20 mouse 76117 Arhgap15 Rho GTPase activating prote... NM_001301832.1 64.2% 63.7% (many diffs)
21 mouse 76117 Arhgap15 Rho GTPase activating prote... XR_001783199.1 45.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1491
ORF length:
1425
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gaaatctaca aattctgata cttccgtgga aacactgaat tctacccgcc
121aaggcacagg agctgtgcaa atgagaatca aaaatgccaa cagccaccat gacaggctca
181gccaaagtaa atccatgatc ctcaccgatg tcgggaaggt cactgaacct atatccagac
241acagaaggaa tcattcacag catatcttga aagatgtcat tcctccattg gaacaactga
301tggttgaaaa agaaggttat ctgcaaaaag ctaaaattgc agatggagga aagaaactaa
361ggaaaaactg gtctacttcc tggattgttc tttctagtcg aagaattgaa ttttacaaag
421aatccaagca acaggctctg tccaatatga aaactgggca caaaccagaa agtgtggatt
481tgtgtggagc acacattgaa tgggccaagg aaaaatcgag cagaaagaat gtctttcaga
541tcacaacagt atcaggaaat gagttccttc tacagtcaga tattgacttc atcatattgg
601attggttcca cgctatcaaa aatgcaattg acagattgcc aaaggattca agttgtccat
661caagaaacct ggaattattc aaaatccaaa gatcctctag cactgaattg ctaagtcact
721acgacagtga tataaaagaa cagaaaccag agcacagaaa atctttaatg ttcagactgc
781atcacagtgc ttccgataca agcgacaaaa atcgagttaa aagcagatta aagaagttta
841ttacccgaag accttccctg aaaactctgc aagaaaaagg acttattaaa gatcaaattt
901ttggctctca tctgcacaaa gtgtgtgaaC GTGAAAATTC CACAGTTCCG TGGTTTGTAA
961AGCAATGCAT TGAAGCTGTT GAGAAAAGAG GTCTAGATGT TGATGGAATA TATCGAGTTA
1021GTGGCAATCT GGCAACAATA CAGAAGTTAA GATTTATTGT CAACCAAGAA GAGAAGCTGA
1081ATTTGGACGA CAGCCAGTGG GAGGACATCC ACGTTGTCAC CGGAGCACTG AAGATGTTTT
1141TCCGGGAGCT GCCTGAGCCG CTCTTCCCTT ACAGTTTCTT TGAGCAGTTT GTGGAAGCGA
1201TCAAAAAGCA AGACAACAAC ACAAGAATTG AAGCTGTAAA ATCTCTTGTA CAAAAACTCC
1261CTCCGCCAAA TCGTGACACC ATGAAAGTCC CCTTTGGACA TCTAACTAAG ATAGTGGCCA
1321AAGCCTCCAA GAACCTCATG TCCACGCAAA GCTTGGGGAT TGTATTTGGA CCTACCCTTC
1381TGCGAGCTGA AAATGAAACA GGAAACATGG CGATCCACAT GGTCTACCAG AACCAGATAG
1441CTGAGCTCAT GCTGAGTGAG TACAGTAAGA TCTTCGGCTC AGAGGAAGAC TGCCCAACTT
1501TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1561CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1621CTTGTGGAAA GGACGAGGCG ATTCGTACCC TCACTGGCGC ACGCGTTAAG TCgacaatca
1681acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)