Construct: ORF TRCN0000474228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005596.1_s317c1
- Derived from:
- ccsbBroadEn_08597
- DNA Barcode:
- GGCGATTCGTACCCTCACTGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP15 (55843)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474228
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | NM_018460.4 | 99.9% | 99.7% | 1226T>C |
| 2 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_011511479.2 | 99.9% | 99.7% | 1226T>C |
| 3 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_017004499.2 | 99.9% | 99.7% | 1226T>C |
| 4 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_011511481.2 | 90.3% | 87.6% | (many diffs) |
| 5 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_017004500.2 | 90.1% | 88.8% | (many diffs) |
| 6 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_011511482.2 | 87.4% | 86.9% | (many diffs) |
| 7 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_011511483.1 | 77.8% | 70.2% | (many diffs) |
| 8 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_011511484.1 | 71.6% | 70% | (many diffs) |
| 9 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_017004501.1 | 69.2% | 66.1% | (many diffs) |
| 10 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XR_001738850.1 | 52.1% | (many diffs) | |
| 11 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_024453000.1 | 50.6% | 50.5% | 0_1ins702;524T>C |
| 12 | human | 55843 | ARHGAP15 | Rho GTPase activating prote... | XM_024453001.1 | 50.6% | 50.5% | 0_1ins702;524T>C |
| 13 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | NM_153820.3 | 86.6% | 84.8% | (many diffs) |
| 14 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XM_006498399.3 | 83.8% | 82.9% | (many diffs) |
| 15 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XM_006498398.3 | 82.6% | 80.7% | (many diffs) |
| 16 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XM_006498400.3 | 79.9% | 75.6% | (many diffs) |
| 17 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XM_006498397.3 | 78.9% | 73.4% | (many diffs) |
| 18 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | NM_001301831.1 | 78.6% | 75.7% | (many diffs) |
| 19 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XM_006498402.3 | 72.5% | 70.4% | (many diffs) |
| 20 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | NM_001301832.1 | 64.2% | 63.7% | (many diffs) |
| 21 | mouse | 76117 | Arhgap15 | Rho GTPase activating prote... | XR_001783199.1 | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaaatctaca aattctgata cttccgtgga aacactgaat tctacccgcc 121 aaggcacagg agctgtgcaa atgagaatca aaaatgccaa cagccaccat gacaggctca 181 gccaaagtaa atccatgatc ctcaccgatg tcgggaaggt cactgaacct atatccagac 241 acagaaggaa tcattcacag catatcttga aagatgtcat tcctccattg gaacaactga 301 tggttgaaaa agaaggttat ctgcaaaaag ctaaaattgc agatggagga aagaaactaa 361 ggaaaaactg gtctacttcc tggattgttc tttctagtcg aagaattgaa ttttacaaag 421 aatccaagca acaggctctg tccaatatga aaactgggca caaaccagaa agtgtggatt 481 tgtgtggagc acacattgaa tgggccaagg aaaaatcgag cagaaagaat gtctttcaga 541 tcacaacagt atcaggaaat gagttccttc tacagtcaga tattgacttc atcatattgg 601 attggttcca cgctatcaaa aatgcaattg acagattgcc aaaggattca agttgtccat 661 caagaaacct ggaattattc aaaatccaaa gatcctctag cactgaattg ctaagtcact 721 acgacagtga tataaaagaa cagaaaccag agcacagaaa atctttaatg ttcagactgc 781 atcacagtgc ttccgataca agcgacaaaa atcgagttaa aagcagatta aagaagttta 841 ttacccgaag accttccctg aaaactctgc aagaaaaagg acttattaaa gatcaaattt 901 ttggctctca tctgcacaaa gtgtgtgaaC GTGAAAATTC CACAGTTCCG TGGTTTGTAA 961 AGCAATGCAT TGAAGCTGTT GAGAAAAGAG GTCTAGATGT TGATGGAATA TATCGAGTTA 1021 GTGGCAATCT GGCAACAATA CAGAAGTTAA GATTTATTGT CAACCAAGAA GAGAAGCTGA 1081 ATTTGGACGA CAGCCAGTGG GAGGACATCC ACGTTGTCAC CGGAGCACTG AAGATGTTTT 1141 TCCGGGAGCT GCCTGAGCCG CTCTTCCCTT ACAGTTTCTT TGAGCAGTTT GTGGAAGCGA 1201 TCAAAAAGCA AGACAACAAC ACAAGAATTG AAGCTGTAAA ATCTCTTGTA CAAAAACTCC 1261 CTCCGCCAAA TCGTGACACC ATGAAAGTCC CCTTTGGACA TCTAACTAAG ATAGTGGCCA 1321 AAGCCTCCAA GAACCTCATG TCCACGCAAA GCTTGGGGAT TGTATTTGGA CCTACCCTTC 1381 TGCGAGCTGA AAATGAAACA GGAAACATGG CGATCCACAT GGTCTACCAG AACCAGATAG 1441 CTGAGCTCAT GCTGAGTGAG TACAGTAAGA TCTTCGGCTC AGAGGAAGAC TGCCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGAGGCG ATTCGTACCC TCACTGGCGC ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt