Construct: ORF TRCN0000474665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007611.1_s317c1
- Derived from:
- ccsbBroadEn_15442
- DNA Barcode:
- AGGGGATGGAGGTTCGACCCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NRG1 (3084)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474665
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3084 | NRG1 | neuregulin 1 | NM_013958.3 | 99.8% | 99.5% | 113G>A |
| 2 | human | 3084 | NRG1 | neuregulin 1 | NM_004495.4 | 87.4% | 87.1% | 113G>A;633_634ins90 |
| 3 | human | 3084 | NRG1 | neuregulin 1 | NM_001160005.1 | 85.7% | 85% | 113G>A;400_401ins102 |
| 4 | human | 3084 | NRG1 | neuregulin 1 | NM_001160002.2 | 80.3% | 79.6% | 113G>A;451_452ins51;582_583ins90 |
| 5 | human | 3084 | NRG1 | neuregulin 1 | NM_001160007.2 | 73.3% | 72.6% | 113G>A;400_401ins102;531_532ins90 |
| 6 | human | 3084 | NRG1 | neuregulin 1 | NM_001160008.2 | 56.9% | 53.8% | (many diffs) |
| 7 | human | 3084 | NRG1 | neuregulin 1 | NM_001160004.3 | 52.1% | 49.3% | (many diffs) |
| 8 | human | 3084 | NRG1 | neuregulin 1 | NM_013960.5 | 50.5% | 45.5% | (many diffs) |
| 9 | human | 3084 | NRG1 | neuregulin 1 | XM_017013372.2 | 47.2% | 40.4% | (many diffs) |
| 10 | human | 3084 | NRG1 | neuregulin 1 | XM_017013371.2 | 45.7% | 37.1% | (many diffs) |
| 11 | human | 3084 | NRG1 | neuregulin 1 | NM_013962.2 | 43.9% | 39% | (many diffs) |
| 12 | human | 3084 | NRG1 | neuregulin 1 | NM_013957.5 | 37.5% | 35.7% | (many diffs) |
| 13 | human | 3084 | NRG1 | neuregulin 1 | NM_013964.5 | 36.5% | 33.1% | (many diffs) |
| 14 | human | 3084 | NRG1 | neuregulin 1 | NM_013956.5 | 36.2% | 35.8% | (many diffs) |
| 15 | human | 3084 | NRG1 | neuregulin 1 | XM_017013366.2 | 34.4% | 29.7% | (many diffs) |
| 16 | human | 3084 | NRG1 | neuregulin 1 | XM_017013365.2 | 33.4% | 27.4% | (many diffs) |
| 17 | human | 3084 | NRG1 | neuregulin 1 | XM_011544512.2 | 33.1% | 29.8% | (many diffs) |
| 18 | human | 3084 | NRG1 | neuregulin 1 | NM_001159999.3 | 32.7% | 31.3% | (many diffs) |
| 19 | human | 3084 | NRG1 | neuregulin 1 | XM_024447143.1 | 31.2% | 27.8% | (many diffs) |
| 20 | human | 3084 | NRG1 | neuregulin 1 | XM_017013368.2 | 30.9% | 30.3% | (many diffs) |
| 21 | human | 3084 | NRG1 | neuregulin 1 | NM_001159995.3 | 30% | 28.5% | (many diffs) |
| 22 | human | 3084 | NRG1 | neuregulin 1 | XM_017013367.1 | 28% | 24.6% | (many diffs) |
| 23 | human | 3084 | NRG1 | neuregulin 1 | NM_001160001.3 | 27.4% | 25.8% | (many diffs) |
| 24 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_017312638.1 | 44.4% | 42% | (many diffs) |
| 25 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509080.3 | 32.3% | 33.1% | (many diffs) |
| 26 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509078.3 | 31.1% | 32% | (many diffs) |
| 27 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509062.3 | 23.7% | 23.1% | (many diffs) |
| 28 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509060.3 | 23.6% | 22.8% | (many diffs) |
| 29 | mouse | 211323 | Nrg1 | neuregulin 1 | XM_006509059.3 | 23.1% | 22.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgagcgcaaa gaaggcagag gcaaagggaa gggcaagaag aaggagcgag 121 gctccggcaa gaagccggag tccgcggcgg gcagccagag cccagccttg cctccccaat 181 tgaaagagat gaaaagccag gaatcggctg caggttccaa actagtcctt cggtgtgaaa 241 ccagttctga atactcctct ctcagattca agtggttcaa gaatgggaat gaattgaatc 301 gaaaaaaCAA ACCACAAAAT ATCAAGATAC AAAAAAAGCC AGGGAAGTCA GAACTTCGCA 361 TTAACAAAGC ATCACTGGCT GATTCTGGAG AGTATATGTG CAAAGTGATC AGCAAATTAG 421 GAAATGACAG TGCCTCTGCC AATATCACCA TCGTGGAATC AAACGAGATC ATCACTGGTA 481 TGCCAGCCTC AACTGAAGGA GCATATGTGT CTTCAGAGTC TCCCATTAGA ATATCAGTAT 541 CCACAGAAGG AGCAAATACT TCTTCATCTA CATCTACATC CACCACTGGG ACAAGCCATC 601 TTGTAAAATG TGCGGAGAAG GAGAAAACTT TCTGTGTGAA TGGAGGGGAG TGCTTCATGG 661 TGAAAGACCT TTCAAACCCC TCGAGATACT TGTGCAAGTG CCCAAATGAG TTTACTGGTG 721 ATCGCTGCCA AAACTACGTA ATGGCCAGCT TCTACAGTAC GTCCACTCCC TTTCTGTCTC 781 TGCCTGAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGGGA TGGAGGTTCG ACCCTCTCAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt