Construct: ORF TRCN0000479044

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012141.1_s317c1
Derived from:
ccsbBroadEn_08957
DNA Barcode:
TGGCGGGCTAAATACACTTAATAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACBD4 (79777)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479044
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135706.3 99.9% 99.7% 10G>N
2 human 79777 ACBD4 acyl-CoA binding domain con... NM_001321352.2 99.9% 99.7% 10G>N
3 human 79777 ACBD4 acyl-CoA binding domain con... NM_001321353.2 99.9% 99.7% 10G>N
4 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025088.1 99.9% 99.7% 10G>N
5 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025087.1 99.6% 99.1% 10G>N;611_613delGCA
6 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025084.1 97.8% 97.4% 10G>N;611_631del
7 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025085.1 97.8% 97.4% 10G>N;611_631del
8 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025086.1 97.8% 97.4% 10G>N;611_631del
9 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135705.3 89.3% 55.4% 10G>N;499_500ins38;915_916ins70
10 human 79777 ACBD4 acyl-CoA binding domain con... NM_024722.4 89.3% 55.4% 10G>N;499_500ins38;915_916ins70
11 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025091.1 89.3% 55.4% 10G>N;499_500ins38;915_916ins70
12 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025092.2 89.3% 55.4% 10G>N;499_500ins38;915_916ins70
13 human 79777 ACBD4 acyl-CoA binding domain con... XM_006722085.2 89% 55.1% (many diffs)
14 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025090.1 87.5% 55.7% (many diffs)
15 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025094.1 82.7% 79.5% (many diffs)
16 human 79777 ACBD4 acyl-CoA binding domain con... XM_024450949.1 82.6% 50.6% (many diffs)
17 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025089.2 82.4% 50.3% (many diffs)
18 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025093.1 80.8% 77.4% (many diffs)
19 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025097.1 79.3% 66.5% 10G>N;685_686ins140;813_814ins70
20 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025098.1 79.3% 66.5% 10G>N;685_686ins140;813_814ins70
21 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025099.1 78.7% 53.4% (many diffs)
22 human 79777 ACBD4 acyl-CoA binding domain con... NM_001135707.3 77.8% 66.2% (many diffs)
23 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025095.1 77.7% 65% (many diffs)
24 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025096.1 77.2% 53.7% (many diffs)
25 human 79777 ACBD4 acyl-CoA binding domain con... XM_011525259.2 72.2% 64% (many diffs)
26 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025100.1 70.8% 62.5% (many diffs)
27 human 79777 ACBD4 acyl-CoA binding domain con... XM_011525261.2 64.9% 46.5% (many diffs)
28 human 79777 ACBD4 acyl-CoA binding domain con... XM_017025101.2 63.6% 45.7% (many diffs)
29 human 79777 ACBD4 acyl-CoA binding domain con... XM_024450950.1 60% 57.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1089
ORF length:
1023
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg caccnagaaa gaaagcccag agcccgactg ccagaaacag ttccaggctg
121cagtgagcgt catccagaac ctgcccaaga acggttctta ccgcccctcc tatgaagaga
181tgctgcgatt ctacagttac tacaagcagg ccaccatggg gccctgcctg gtcccccggc
241ccgggttctg ggaccccatt ggacgatata agtgggacgc ctggaacagt ctgggcaaga
301tgagcaggga ggaggccatg tctgcctaca tcactgaaat gaaactggtg gcacagaagg
361tgatcgacac agtgcccctg ggtgaggtgg cagaggacat gtttggttac ttcgagcccc
421tgtaccaggt gatccctgac atgccgaggc ccccagagac cttcctgaga agggtcacag
481gttggaaaga gcaggttgtg aatggagatg ttggggctgt ttcagagcct ccctgcctcc
541ccaaggaacc ggcaccccca agcccagctt ccctctgggc agtaactcta ccaacccctc
601cacagagtcc cattcaccca gggacctgga ctccgaggtt ttctgtgatt ccctggagca
661gctggagcct gagctggttt GGACAGAGCA GCGGGCAGCA TCTGGAGGAA AGCGTGATCC
721CAGGAACAGC CCCGTGCCCC CCACAAAGAA AGAGGGGTTG CGGGGCAGCC CGCCGGGGCC
781CCAGGAGTTG GACGTGTGGC TGCTGGGGAC AGTTCGAGCA CTACAGGAGA GCATGCAGGA
841GGTGCAGGCG AGGGTGCAGA GCCTGGAGAG CATGCCCCGG CCCCCTGAGC AGAGGCCGCA
901GCCCAGGCCC AGTGCTCGGC CATGGCCCCT TGGGCTCCCG GGGCCCGCGC TGCTCTTCTT
961CCTCCTGTGG CCCTTCGTCG TCCAGTGGCT CTTCCGAATG TTTCGGACCC AAAAGAGGTG
1021ACTGTCAGTG GAGGGGTCTC TGCAGCCAAC TGAGACTATC TTGCTGTGCC CTGAGCCTTC
1081CTAGGGTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1141CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1201TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGGCGG GCTAAATACA CTTAATACAC
1261GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)