Construct: ORF TRCN0000479236

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006828.2_s317c1
Derived from:
ccsbBroadEn_14687
DNA Barcode:
CTGTCAGATCCGTACCTGTCATTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KIR2DS4 (3809)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479236
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3806 KIR2DS1 killer cell immunoglobulin ... NM_014512.1 97.6% 82.2% (many diffs)
2 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_011547998.2 97.6% 82.2% (many diffs)
3 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_012314.6 97.3% 82.2% (many diffs)
4 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_012312.4 95.8% 79.9% (many diffs)
5 human 100132285 KIR2DS2 killer cell immunoglobulin ... XM_017030275.2 95.8% 79.9% (many diffs)
6 human 3810 KIR2DS5 killer cell immunoglobulin ... NM_014513.2 94.9% 78.2% (many diffs)
7 human 102725023 LOC102725023 killer cell immunoglobulin-... NM_001360171.1 94.6% 77.6% (many diffs)
8 human 3808 KIR2DS3 killer cell immunoglobulin ... NM_012313.1 94.6% 77.6% (many diffs)
9 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291701.1 90.3% 74.6% (many diffs)
10 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282170.1 85.2% 74.9% (many diffs)
11 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291695.1 84.3% 71.8% (many diffs)
12 human 3804 KIR2DL3 killer cell immunoglobulin ... NM_015868.3 84.3% 70% (many diffs)
13 human 112267881 LOC112267881 killer cell immunoglobulin-... NM_001368251.1 82.9% 70.2% (many diffs)
14 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452542.1 82.6% 78.3% (many diffs)
15 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282171.1 82.2% 72.8% (many diffs)
16 human 3802 KIR2DL1 killer cell immunoglobulin ... NM_014218.3 82.2% 68.7% (many diffs)
17 human 3803 KIR2DL2 killer cell immunoglobulin ... NM_014219.2 81.7% 68.4% (many diffs)
18 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452543.1 81% 67.1% (many diffs)
19 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452544.1 81% 67.1% (many diffs)
20 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452545.1 81% 67.1% (many diffs)
21 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026783.1 76.5% 77.7% (many diffs)
22 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281971.2 76.1% 57.3% (many diffs)
23 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_017030286.1 73.1% 75.3% (many diffs)
24 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_011526939.2 72.3% 73.5% (many diffs)
25 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281972.2 70.6% 60% (many diffs)
26 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026782.1 67.8% 55.5% (many diffs)
27 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001083539.2 65.1% 57% (many diffs)
28 human 112268355 LOC112268355 killer cell immunoglobulin-... NM_001368254.1 65.1% 57% (many diffs)
29 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291696.1 64.8% 50.6% (many diffs)
30 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_001322168.1 61.9% 48.8% (many diffs)
31 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_013289.2 61.7% 48.4% (many diffs)
32 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_006737.4 60.7% 48.3% (many diffs)
33 human 115653 KIR3DL3 killer cell immunoglobulin ... NM_153443.4 59.1% 44% (many diffs)
34 human 3811 KIR3DL1 killer cell immunoglobulin ... XM_017030274.1 57.5% 49.4% (many diffs)
35 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_001242867.2 57.3% 45.2% (many diffs)
36 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_017026784.2 56% 54.5% (many diffs)
37 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_011526940.3 54.3% 49.7% (many diffs)
38 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291700.1 32% 20.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
897
ORF length:
828
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gttgctcatg gtcatcagca tggcgtgtgt tgggttcttc ttgctgcagg
121gggcctggcc acaggaggga gtccacagaa aaccttcctt cctggccctc ccaggtcacc
181tggtgaaatc agaagagaca gtcatcctgc aatgttggtc agatgtcatg tttgaacact
241tccttctgca cagagagggg atgtttaacg acactttgca cctcattgga gagcaccatg
301atggggtttc caaggccaac ttctccattg gtcccatgat gcctgtcctt gcaggaacct
361acagatgcta cggttctgtt cctcactccc cctatcagtt gtcagctccc agtgaccctc
421tggacatcgt gatcataggt ctatatgaga aaccttctct ctcagcccag ccgggcccca
481cggttctggc aggagagaat gtgaCCTTGT CCTGCAGCTC CCGGAGCTCC TATGACATGT
541ACCATCTATC CAGGGAAGGG GAGGCCCATG AACGTAGGCT CCCTGCAGGG ACCAAGGTCA
601ACGGAACATT CCAGGCCAAC TTTCCTCTGG GCCCTGCCAC CCATGGAGGG ACCTACAGAT
661GCTTCGGCTC TTTCCGTGAC TCTCCATACG AGTGGTCAAA GTCAAGTGAC CCACTGCTTG
721TTTCTGTCAC AGGAAACCCT TCAAATAGTT GGCCTTCACC CACTGAACCA AGCTCCGAAA
781CCGGTAACCC CAGACACCTA CATGTTCTGA TTGGGACCTC AGTGGTCAAA ATCCCTTTCA
841CCATCCTCCT CTCTTTCTCC TTCATCGCTG GTGCTCCGAC AAAAAAAATG CTGCTGTAAT
901GGACCAAGAG CCTGCAGGGA ACAGAACAGT GAACAGCGAG GATTCTGATG AACAAGACCA
961TCAGGAGGTG TCATACGCAT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
1021TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
1081AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTGTC AGATCCGTAC
1141CTGTCATTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)