Construct: ORF TRCN0000479468

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002949.1_s317c1
Derived from:
ccsbBroadEn_13888
DNA Barcode:
TCAGTAGCAAGTTATTCAATCTAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
KIR2DS4 (3809)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479468
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_012314.6 99.6% 4.8% 10_11insC;16A>G;20T>G
2 human 3806 KIR2DS1 killer cell immunoglobulin ... NM_014512.1 95.7% 3.3% (many diffs)
3 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_012312.4 95.7% 3% (many diffs)
4 human 100132285 KIR2DS2 killer cell immunoglobulin ... XM_017030275.2 95.7% 3% (many diffs)
5 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_011547998.2 95.6% 3.3% (many diffs)
6 human 3810 KIR2DS5 killer cell immunoglobulin ... NM_014513.2 93.7% 3.3% (many diffs)
7 human 102725023 LOC102725023 killer cell immunoglobulin-... NM_001360171.1 93.6% 3% (many diffs)
8 human 3808 KIR2DS3 killer cell immunoglobulin ... NM_012313.1 93.6% 3% (many diffs)
9 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291701.1 90.1% 6.1% (many diffs)
10 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282170.1 85.3% 4.2% (many diffs)
11 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291695.1 84.2% 2.7% (many diffs)
12 human 3804 KIR2DL3 killer cell immunoglobulin ... NM_015868.3 83.8% 3% (many diffs)
13 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001282171.1 82.3% 6.2% (many diffs)
14 human 3803 KIR2DL2 killer cell immunoglobulin ... NM_014219.2 81.5% 2.7% (many diffs)
15 human 3802 KIR2DL1 killer cell immunoglobulin ... NM_014218.3 81.2% 2.7% (many diffs)
16 human 112267881 LOC112267881 killer cell immunoglobulin-... NM_001368251.1 81.1% 2.9% (many diffs)
17 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452542.1 80.5% 3.5% (many diffs)
18 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452543.1 78.8% 4% (many diffs)
19 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452544.1 78.8% 4% (many diffs)
20 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_024452545.1 78.8% 4% (many diffs)
21 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281971.2 78.3% 6% (many diffs)
22 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026783.1 75.2% 5.9% (many diffs)
23 human 3809 KIR2DS4 killer cell immunoglobulin ... NM_001281972.2 72.7% 6.5% (many diffs)
24 human 3806 KIR2DS1 killer cell immunoglobulin ... XM_017030286.1 71% 7% (many diffs)
25 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_011526939.2 70.8% 7% (many diffs)
26 human 3802 KIR2DL1 killer cell immunoglobulin ... XM_017026782.1 66.7% 3.1% (many diffs)
27 human 3813 KIR3DS1 killer cell immunoglobulin ... NM_001083539.2 65.3% 3.2% (many diffs)
28 human 112268355 LOC112268355 killer cell immunoglobulin-... NM_001368254.1 65.3% 3.2% (many diffs)
29 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291696.1 64.8% 4.4% (many diffs)
30 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_001322168.1 62% 3% (many diffs)
31 human 3811 KIR3DL1 killer cell immunoglobulin ... NM_013289.2 61.9% 2.1% (many diffs)
32 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_006737.4 60.8% 3.4% (many diffs)
33 human 115653 KIR3DL3 killer cell immunoglobulin ... NM_153443.4 59.2% 3.5% (many diffs)
34 human 3812 KIR3DL2 killer cell immunoglobulin ... NM_001242867.2 57.4% 3.6% (many diffs)
35 human 3811 KIR3DL1 killer cell immunoglobulin ... XM_017030274.1 57.1% 3.9% (many diffs)
36 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_017026784.2 56% 4.6% (many diffs)
37 human 3812 KIR3DL2 killer cell immunoglobulin ... XM_011526940.3 54.3% 3.7% (many diffs)
38 human 100132285 KIR2DS2 killer cell immunoglobulin ... NM_001291700.1 32.1% 12.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
234
ORF length:
165
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgctcact ggtcgtcagc atggcgtgtg ttgggttctt cttgctgcag
121ggggcctggc cacaggaggg agtccacaga aaaccttcct tcctggccct cccaggtcac
181ctggtgaaat cagaagagac agtcatcctg caatgttggt cggatgtcat gtttgagcac
241ttccttctgc acagagaggg gaagtttaac aacactttgc acctcattgg agagcaccat
301gatggggttt ccaaggccaa cttctccatt ggtcccatga tgcctgtcct tgcaggaacc
361tacagatgct acggttctgt tcctcactcc ccctatcagt tgtcagctcc cagtgaccct
421ctggacatgg tgatcatagg tctatatgag aaaccttctc tctcagccca gccgggcccc
481acggttcagg caggagagaa tgtgaccttg tcctgcagct cccggagctc ctatgacatg
541taccatctat ccagggaagg ggaggcccat gaacgtaggc tccctgcagt gcgcagcatc
601aacggaacat tccaggccga ctttcctctg ggccctgcca cccacggagg gacctacaga
661tgcttcggct ctttccgtga cgctcccTAC GAGTGGTCAA ACTCGAGTGA TCCACTGCTT
721GTTTCCGTCA CAGGAAACCC TTCAAATAGT TGGCCTTCAC CCACTGAACC AAGCTCCAAA
781ACCGGTAACC CCAGACACCT ACATGTTCTG ATTGGGACCT CAGTGGTCAA AATCCCTTTC
841ACCATCCTCC TCTTCTTTCT CCTTCATCGC TGGTGCTCCG ACAAAAAAAA TGCTGCTGTA
901ATGGACCAAG AGCCTGCAGG GAACAGAACA GTGAACAGCG AGGATTCTGA TGAACAAGAC
961CATCAGGAGG TGTCATACGC ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG
1021CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT
1081GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA GTAGCAAGTT
1141ATTCAATCTA GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat
1201t

Download FASTA (ORF) (Full)