Construct: ORF TRCN0000479468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002949.1_s317c1
- Derived from:
- ccsbBroadEn_13888
- DNA Barcode:
- TCAGTAGCAAGTTATTCAATCTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KIR2DS4 (3809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_012314.6 | 99.6% | 4.8% | 10_11insC;16A>G;20T>G |
2 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | NM_014512.1 | 95.7% | 3.3% | (many diffs) |
3 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_012312.4 | 95.7% | 3% | (many diffs) |
4 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | XM_017030275.2 | 95.7% | 3% | (many diffs) |
5 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_011547998.2 | 95.6% | 3.3% | (many diffs) |
6 | human | 3810 | KIR2DS5 | killer cell immunoglobulin ... | NM_014513.2 | 93.7% | 3.3% | (many diffs) |
7 | human | 102725023 | LOC102725023 | killer cell immunoglobulin-... | NM_001360171.1 | 93.6% | 3% | (many diffs) |
8 | human | 3808 | KIR2DS3 | killer cell immunoglobulin ... | NM_012313.1 | 93.6% | 3% | (many diffs) |
9 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291701.1 | 90.1% | 6.1% | (many diffs) |
10 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001282170.1 | 85.3% | 4.2% | (many diffs) |
11 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291695.1 | 84.2% | 2.7% | (many diffs) |
12 | human | 3804 | KIR2DL3 | killer cell immunoglobulin ... | NM_015868.3 | 83.8% | 3% | (many diffs) |
13 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001282171.1 | 82.3% | 6.2% | (many diffs) |
14 | human | 3803 | KIR2DL2 | killer cell immunoglobulin ... | NM_014219.2 | 81.5% | 2.7% | (many diffs) |
15 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | NM_014218.3 | 81.2% | 2.7% | (many diffs) |
16 | human | 112267881 | LOC112267881 | killer cell immunoglobulin-... | NM_001368251.1 | 81.1% | 2.9% | (many diffs) |
17 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452542.1 | 80.5% | 3.5% | (many diffs) |
18 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452543.1 | 78.8% | 4% | (many diffs) |
19 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452544.1 | 78.8% | 4% | (many diffs) |
20 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_024452545.1 | 78.8% | 4% | (many diffs) |
21 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_001281971.2 | 78.3% | 6% | (many diffs) |
22 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_017026783.1 | 75.2% | 5.9% | (many diffs) |
23 | human | 3809 | KIR2DS4 | killer cell immunoglobulin ... | NM_001281972.2 | 72.7% | 6.5% | (many diffs) |
24 | human | 3806 | KIR2DS1 | killer cell immunoglobulin ... | XM_017030286.1 | 71% | 7% | (many diffs) |
25 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_011526939.2 | 70.8% | 7% | (many diffs) |
26 | human | 3802 | KIR2DL1 | killer cell immunoglobulin ... | XM_017026782.1 | 66.7% | 3.1% | (many diffs) |
27 | human | 3813 | KIR3DS1 | killer cell immunoglobulin ... | NM_001083539.2 | 65.3% | 3.2% | (many diffs) |
28 | human | 112268355 | LOC112268355 | killer cell immunoglobulin-... | NM_001368254.1 | 65.3% | 3.2% | (many diffs) |
29 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291696.1 | 64.8% | 4.4% | (many diffs) |
30 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | NM_001322168.1 | 62% | 3% | (many diffs) |
31 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | NM_013289.2 | 61.9% | 2.1% | (many diffs) |
32 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | NM_006737.4 | 60.8% | 3.4% | (many diffs) |
33 | human | 115653 | KIR3DL3 | killer cell immunoglobulin ... | NM_153443.4 | 59.2% | 3.5% | (many diffs) |
34 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | NM_001242867.2 | 57.4% | 3.6% | (many diffs) |
35 | human | 3811 | KIR3DL1 | killer cell immunoglobulin ... | XM_017030274.1 | 57.1% | 3.9% | (many diffs) |
36 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | XM_017026784.2 | 56% | 4.6% | (many diffs) |
37 | human | 3812 | KIR3DL2 | killer cell immunoglobulin ... | XM_011526940.3 | 54.3% | 3.7% | (many diffs) |
38 | human | 100132285 | KIR2DS2 | killer cell immunoglobulin ... | NM_001291700.1 | 32.1% | 12.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 234
- ORF length:
- 165
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgctcact ggtcgtcagc atggcgtgtg ttgggttctt cttgctgcag 121 ggggcctggc cacaggaggg agtccacaga aaaccttcct tcctggccct cccaggtcac 181 ctggtgaaat cagaagagac agtcatcctg caatgttggt cggatgtcat gtttgagcac 241 ttccttctgc acagagaggg gaagtttaac aacactttgc acctcattgg agagcaccat 301 gatggggttt ccaaggccaa cttctccatt ggtcccatga tgcctgtcct tgcaggaacc 361 tacagatgct acggttctgt tcctcactcc ccctatcagt tgtcagctcc cagtgaccct 421 ctggacatgg tgatcatagg tctatatgag aaaccttctc tctcagccca gccgggcccc 481 acggttcagg caggagagaa tgtgaccttg tcctgcagct cccggagctc ctatgacatg 541 taccatctat ccagggaagg ggaggcccat gaacgtaggc tccctgcagt gcgcagcatc 601 aacggaacat tccaggccga ctttcctctg ggccctgcca cccacggagg gacctacaga 661 tgcttcggct ctttccgtga cgctcccTAC GAGTGGTCAA ACTCGAGTGA TCCACTGCTT 721 GTTTCCGTCA CAGGAAACCC TTCAAATAGT TGGCCTTCAC CCACTGAACC AAGCTCCAAA 781 ACCGGTAACC CCAGACACCT ACATGTTCTG ATTGGGACCT CAGTGGTCAA AATCCCTTTC 841 ACCATCCTCC TCTTCTTTCT CCTTCATCGC TGGTGCTCCG ACAAAAAAAA TGCTGCTGTA 901 ATGGACCAAG AGCCTGCAGG GAACAGAACA GTGAACAGCG AGGATTCTGA TGAACAAGAC 961 CATCAGGAGG TGTCATACGC ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 1021 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 1081 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA GTAGCAAGTT 1141 ATTCAATCTA GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 1201 t