Construct: ORF TRCN0000480321

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012326.1_s317c1
Derived from:
ccsbBroadEn_05670
DNA Barcode:
TTAGTTTTGGATGACTTAGATTGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSG8 (440533)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480321
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440533 PSG8 pregnancy specific beta-1-g... NM_182707.3 100% 100%
2 human 440533 PSG8 pregnancy specific beta-1-g... NM_001130167.2 98% 97.4% (many diffs)
3 human 5669 PSG1 pregnancy specific beta-1-g... NM_006905.3 95.7% 92.4% (many diffs)
4 human 5669 PSG1 pregnancy specific beta-1-g... NM_001297773.2 94.4% 90.1% (many diffs)
5 human 5672 PSG4 pregnancy specific beta-1-g... XM_017026997.2 94.2% 89.6% (many diffs)
6 human 5669 PSG1 pregnancy specific beta-1-g... NM_001184825.2 94.1% 90.6% (many diffs)
7 human 5669 PSG1 pregnancy specific beta-1-g... NM_001184826.2 93.8% 90.3% (many diffs)
8 human 5671 PSG3 pregnancy specific beta-1-g... NM_021016.4 93.8% 87.9% (many diffs)
9 human 5676 PSG7 pregnancy specific beta-1-g... NM_002783.3 93.1% 88.7% (many diffs)
10 human 5672 PSG4 pregnancy specific beta-1-g... NM_002780.5 92.4% 87.5% (many diffs)
11 human 5675 PSG6 pregnancy specific beta-1-g... NM_001031850.4 92% 85.8% (many diffs)
12 human 5678 PSG9 pregnancy specific beta-1-g... NM_002784.5 91% 83.9% (many diffs)
13 human 5675 PSG6 pregnancy specific beta-1-g... NM_002782.5 90.9% 84.8% (many diffs)
14 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027007.2 90.4% 82.5% (many diffs)
15 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027004.2 80.3% 73.9% (many diffs)
16 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526986.1 76.2% 75.3% (many diffs)
17 human 5669 PSG1 pregnancy specific beta-1-g... XM_005259065.4 75% 72% (many diffs)
18 human 5669 PSG1 pregnancy specific beta-1-g... XM_011527125.2 74.2% 71.1% (many diffs)
19 human 5672 PSG4 pregnancy specific beta-1-g... XM_017027001.2 73.8% 70.4% (many diffs)
20 human 5672 PSG4 pregnancy specific beta-1-g... XM_017027002.2 73.4% 69.2% (many diffs)
21 human 5669 PSG1 pregnancy specific beta-1-g... NM_001330524.2 73.3% 70.1% (many diffs)
22 human 5673 PSG5 pregnancy specific beta-1-g... NM_001130014.2 72% 66.7% (many diffs)
23 human 5673 PSG5 pregnancy specific beta-1-g... NM_002781.4 72% 66.7% (many diffs)
24 human 5673 PSG5 pregnancy specific beta-1-g... XM_017027003.1 72% 66.7% (many diffs)
25 human 5672 PSG4 pregnancy specific beta-1-g... NM_001276495.1 72% 68.3% (many diffs)
26 human 5672 PSG4 pregnancy specific beta-1-g... NM_213633.3 71.6% 67.1% (many diffs)
27 human 5670 PSG2 pregnancy specific beta-1-g... NM_031246.4 71.5% 65.8% (many diffs)
28 human 5680 PSG11 pregnancy specific beta-1-g... NM_002785.3 71% 64.7% (many diffs)
29 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301707.2 70.9% 64.8% (many diffs)
30 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301708.2 70.6% 63.8% (many diffs)
31 human 440533 PSG8 pregnancy specific beta-1-g... NM_001130168.2 69.4% 68.5% (many diffs)
32 human 5671 PSG3 pregnancy specific beta-1-g... XM_011527126.2 69.1% 63.9% (many diffs)
33 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526987.3 67.8% 64.7% (many diffs)
34 human 5676 PSG7 pregnancy specific beta-1-g... NM_001206650.2 66.1% 62.6% (many diffs)
35 human 5672 PSG4 pregnancy specific beta-1-g... NM_001316339.2 65.9% 62.2% (many diffs)
36 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027005.2 62.8% 57.3% (many diffs)
37 human 5678 PSG9 pregnancy specific beta-1-g... XM_005259075.4 62.6% 56.5% (many diffs)
38 human 440533 PSG8 pregnancy specific beta-1-g... XM_011526988.2 60.2% 59.6% (many diffs)
39 human 5671 PSG3 pregnancy specific beta-1-g... XM_011527127.2 51.8% 47.7% (many diffs)
40 human 5678 PSG9 pregnancy specific beta-1-g... NM_001301709.2 50.6% 45.2% (many diffs)
41 human 653492 PSG10P pregnancy specific beta-1-g... NR_026824.1 45.8% (many diffs)
42 human 5678 PSG9 pregnancy specific beta-1-g... XM_017027006.2 45.1% 40.4% (many diffs)
43 human 5680 PSG11 pregnancy specific beta-1-g... NM_001113410.2 44.3% 38.7% (many diffs)
44 human 5680 PSG11 pregnancy specific beta-1-g... NM_203287.2 44.3% 38.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1347
ORF length:
1278
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggggctcctc tcagcccctc cctgcacaca gcgcatcacc tggaaggggc
121tcctgctcac agcatcactt ttaaacttct ggaacccacc cacgactgcc caagtcacga
181ttgaagccca gccaaccaaa gtttctgagg ggaaggatgt tcttctactt gtccacaatt
241tgccccagaa tcttactggc tacatctggt acaaagggca aatcagggac ctctaccatt
301acattacatc atatgtagta gacggtcaaa taattatata tgggcctgca tacagtggac
361gagaaacaat atattccaat gcatccctgc tgatccagaa tgtcacccag gaagacgcag
421gatcctacac cttacacatc ataatgggag gtgatgagaa tagaggagta actggacatt
481tcaccttcac cttatatctg gagactccca agccctccat ctccagcagc aaattaaacc
541ccagggaggc catggaggct gtgagcttaa cctgtgatcc tgagactccg gacgcaagct
601acctgtggtg gatgaatggt cagagcctcc ctatgtctca caggttgcag ttgtctgaaa
661ccaacaggac cctctttcta ttgggtgtca caaagtacac tgcaggaccc tatgaatgtg
721aaatacggaa cccagtgagt gccagccgca gtgacccatt caccctgaat ctcctcccga
781agctgcccaa gccctacatc accatcaaca acttaaaacc cagggagaat aaggatgtct
841taaacttcac ctgtgaacct aagagtgaga actacaccta catttggtgg ctaaatggtc
901agagcctccc ggtcagtccc agggtaaagc gacccattga aaacaggaTC CTCATTCTAC
961CCAGTGTCAC GAGAAATGAA ACAGGACCCT ATCAATGTGA AATAAGGGAC CAATATGGTG
1021GCATCCGCAG TTACCCAGTC ACCCTGAATG TCCTCTATGG TCCAGACCTC CCCAGAATTT
1081ACCCTTCATT CACCTATTAC CGTTCAGGAG AAGTCCTCTA CTTGTCCTGT TCTGCGGACT
1141CTAACCCACC GGCACAGTAT TCTTGGACAA TTAATGGGAA GTTTCAGCTA TCAGGACAAA
1201AGCTCTTTAT CCCCCAAATT ACTACAAAGC ATAGCGGGCT CTATGCTTGC TCTGTTCGTA
1261ACTCAGCCAC TGGCAAGGAA AGCTCCAAAT CCATGACAGT AAAAGTCTCT GGTAAGCGGA
1321TCCCAGTATC CTTGGCAATA GGGATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
1381GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1441AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTAGTTTT
1501GGATGACTTA GATTGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1561aagatt

Download FASTA (ORF) (Full)