Construct: ORF TRCN0000488651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019987.1_s317c1
- DNA Barcode:
- AGCATCTACCCAGGGCGTAGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR4 (3360)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_000870.6 | 99.9% | 99.7% | 1164_1165insG |
| 2 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_199453.3 | 94.3% | 93% | (many diffs) |
| 3 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_001040169.2 | 92.6% | 92.6% | (many diffs) |
| 4 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_001040172.2 | 92.4% | 92.2% | 1077_1079delATTinsGGA;1080_1081ins85 |
| 5 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_001040173.2 | 90.5% | 90.4% | 1076_1195del;1284_1285insG |
| 6 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NM_001286410.1 | 90.1% | 82.9% | (many diffs) |
| 7 | human | 3360 | HTR4 | 5-hydroxytryptamine receptor 4 | NR_104445.1 | 32.5% | 1_513del;1588_1834del;1925_3578delinsG | |
| 8 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | NM_008313.4 | 90.8% | 93% | (many diffs) |
| 9 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525676.3 | 90.8% | 93% | (many diffs) |
| 10 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525678.3 | 90.8% | 93% | (many diffs) |
| 11 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_017317839.1 | 90.8% | 93% | (many diffs) |
| 12 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525680.3 | 85.7% | 86.9% | (many diffs) |
| 13 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_011246848.2 | 85.6% | 87.6% | (many diffs) |
| 14 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525681.3 | 85.6% | 84.3% | (many diffs) |
| 15 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525682.3 | 85.6% | 84.3% | (many diffs) |
| 16 | mouse | 15562 | Htr4 | 5 hydroxytryptamine (seroto... | XM_006525683.3 | 85% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1239
- ORF length:
- 1167
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacaaa cttgatgcta atgtgagttc tgaggagggt ttcgggtcag 121 tggagaaggt ggtgctgctc acgtttctct cgacggttat cctgatggcc atcttgggga 181 acctgctggt gatggtggct gtgtgctggg acaggcagct caggaaaata aaaacaaatt 241 atttcattgt atctcttgct tttgcggatc tgctggtttc ggtgctggtg atgccctttg 301 gtgccattga gctggttcaa gacatctgga tttatgggga ggtgttttgt cttgttcgga 361 catctctgga cgtcctgctc acaacggcat cgatttttca cctgtgctgc atttctctgg 421 ataggtatta cgccatctgc tgccagcctt tggtctatag gaacaagatg acccctctgc 481 gcatcgcatt aatgctggga ggctgctggg tcatccccac gtttatttct tttctcccta 541 taatgcaagg ctggaataac attggcataa ttgatttgat agaaaagagg aagttcaacc 601 agaactctaa ctctacgtac tgtgtcttca tggtcaacaa gccctacgcc atcacctgct 661 ctgtggtggc cttctacatc ccatttctcc tcatggtgct ggcctattac cgcatctatg 721 tcacagctaa ggagcatgcc catcagatcc agatgttaca acgggcagga gcctcctccg 781 agagcaggcc tcagtcggca gaccagcata gcactcatcg catgaggaca gagaccaaag 841 cagccaagac cctgtgcatc atcatgggtt gcttctgccT CTGCTGGGCA CCATTCTTTG 901 TCACCAATAT TGTGGATCCT TTCATAGACT ACACTGTCCC TGGGCAGGTG TGGACTGCTT 961 TCCTCTGGCT CGGCTATATC AATTCCGGGT TGAACCCTTT TCTCTACGCC TTCTTGAATA 1021 AGTCTTTTAG ACGTGCCTTC CTCATCATCC TCTGCTGTGA TGATGAGCGC TACCGAAGAC 1081 CTTCCATTCT GGGCCAGACT GTCCCTTGTT CAACCACAAC CATTAATGGA TCCACACATG 1141 TACTAAGGGA TGCAGTGGAG TGTGGTGGCC AGTGGGAGAG TCAGTGTCAC CCGCCAGCAA 1201 CTTCTCCTTT GGTGGCTGCT CAGCCCAGTG ACACTGACCC AGCTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AAGCATCTAC CCAGGGCGTA GTTTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt