Construct: ORF TRCN0000488893

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021036.1_s317c1
DNA Barcode:
GTTCTTTGTCAGGGTACTATTGAA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
HTR4 (3360)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488893
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_000870.6 100% 100%
2 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_199453.3 94.2% 93.3% (many diffs)
3 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_001040169.2 92.6% 90% (many diffs)
4 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_001040172.2 92.5% 92.5% 1077_1079delATTinsGGA;1080_1081ins84
5 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_001040173.2 90.6% 90.6% 1076_1195del
6 human 3360 HTR4 5-hydroxytryptamine receptor 4 NM_001286410.1 89.9% 85.3% (many diffs)
7 human 3360 HTR4 5-hydroxytryptamine receptor 4 NR_104445.1 32.5% 1_513del;1588_1834del;1925_3578del
8 mouse 15562 Htr4 5 hydroxytryptamine (seroto... NM_008313.4 90.8% 93.2% (many diffs)
9 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525676.3 90.8% 93.2% (many diffs)
10 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525678.3 90.8% 93.2% (many diffs)
11 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_017317839.1 90.8% 93.2% (many diffs)
12 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_011246848.2 85.7% 87.8% (many diffs)
13 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525681.3 85.7% 84.5% (many diffs)
14 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525682.3 85.7% 84.5% (many diffs)
15 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525680.3 85.6% 84.5% (many diffs)
16 mouse 15562 Htr4 5 hydroxytryptamine (seroto... XM_006525683.3 84.9% 85.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1236
ORF length:
1164
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggacaaa cttgatgcta atgtgagttc tgaggagggt ttcgggtcag
121tggagaaggt ggtgctgctc acgtttctct cgacggttat cctgatggcc atcttgggga
181acctgctggt gatggtggct gtgtgctggg acaggcagct caggaaaata aaaacaaatt
241atttcattgt atctcttgct tttgcggatc tgctggtttc ggtgctggtg atgccctttg
301gtgccattga gctggttcaa gacatctgga tttatgggga ggtgttttgt cttgttcgga
361catctctgga cgtcctgctc acaacggcat cgatttttca cctgtgctgc atttctctgg
421ataggtatta cgccatctgc tgccagcctt tggtctatag gaacaagatg acccctctgc
481gcatcgcatt aatgctggga ggctgctggg tcatccccac gtttatttct tttctcccta
541taatgcaagg ctggaataac attggcataa ttgatttgat agaaaagagg aagttcaacc
601agaactctaa ctctacgtac tgtgtcttca tggtcaacaa gccctacgcc atcacctgct
661ctgtggtggc cttctacatc ccatttctcc tcatggtgct ggcctattac cgcatctatg
721tcacagctaa ggagcatgcc catcagatcc agatgttaca acgggcagga gcctcctccg
781agagcaggcc tcagtcggca gaccagcata gcactcatcg catgaggaca gagaccaaag
841cagccaagac cctgtgcatc atcatgggtt gcttctgcct ctgctgggca ccattctttG
901TCACCAATAT TGTGGATCCT TTCATAGACT ACACTGTCCC TGGGCAGGTG TGGACTGCTT
961TCCTCTGGCT CGGCTATATC AATTCCGGGT TGAACCCTTT TCTCTACGCC TTCTTGAATA
1021AGTCTTTTAG ACGTGCCTTC CTCATCATCC TCTGCTGTGA TGATGAGCGC TACCGAAGAC
1081CTTCCATTCT GGGCCAGACT GTCCCTTGTT CAACCACAAC CATTAATGGA TCCACACATG
1141TACTAAGGGA TGCAGTGGAG TGTGGTGGCC AGTGGGAGAG TCAGTGTCAC CCGCCAGCAA
1201CTTCTCCTTT GGTGGCTGCT CAGCCCAGTG ACACTTAGAA CCCAGCTTTC TTGTACAAAG
1261TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1321GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1381ACGAGTTCTT TGTCAGGGTA CTATTGAAAC GCGTTAAGTC gacaatcaac ctctggatta
1441caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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