Construct: ORF TRCN0000488922

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021888.1_s317c1
DNA Barcode:
TAACTAAGCCACAGTTTAACTCGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
TUBB3 (10381)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488922
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 100% 100%
2 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 87.8% 91.1% (many diffs)
3 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 87.8% 90.8% (many diffs)
4 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 87.8% 90.8% (many diffs)
5 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 87.7% 91.5% (many diffs)
6 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 87.7% 91.5% (many diffs)
7 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 87.3% 91.3% (many diffs)
8 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 84% 91.7% (many diffs)
9 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 84% 84% 0_1ins216
10 human 203068 TUBB tubulin beta class I NM_178014.4 81.8% 91.3% (many diffs)
11 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 81.8% 84% (many diffs)
12 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 80% 85.3% (many diffs)
13 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 79.8% 82.6% (many diffs)
14 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 78.9% 81.6% (many diffs)
15 human 203068 TUBB tubulin beta class I NM_001293212.2 77.3% 84.4% (many diffs)
16 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 75.1% 77.1% (many diffs)
17 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 75.1% 77.1% (many diffs)
18 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 74.7% 77.3% (many diffs)
19 human 203068 TUBB tubulin beta class I NM_001293214.2 74.2% 83.1% (many diffs)
20 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 74% 71.9% (many diffs)
21 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 72.7% 73% (many diffs)
22 human 203068 TUBB tubulin beta class I NM_001293215.2 68.9% 76.8% (many diffs)
23 human 203068 TUBB tubulin beta class I NM_001293216.2 68.9% 76.8% (many diffs)
24 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 68.1% 72.5% (many diffs)
25 human 260334 TUBB8B tubulin beta 8B XM_024451145.1 67.6% 71.4% (many diffs)
26 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 60.8% 62% (many diffs)
27 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 60.8% 62% (many diffs)
28 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 60.5% 64.6% (many diffs)
29 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 60.5% 64.6% (many diffs)
30 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 59.9% 63.7% (many diffs)
31 human 203068 TUBB tubulin beta class I NM_001293213.2 44.9% 48.4% (many diffs)
32 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 90.7% 99.7% (many diffs)
33 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 86.8% 91.7% (many diffs)
34 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 84.2% 91.3% (many diffs)
35 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 83.6% 91.1% (many diffs)
36 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 82.9% 90.8% (many diffs)
37 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 82.6% 91.3% (many diffs)
38 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 81.7% 91.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1419
ORF length:
1350
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gagggagatc gtgcacatcc aggccggcca gtgcggcaac cagatcgggg
121ccaagttctg ggaagtcatc agtgatgagc atggcatcga ccccagcggc aactacgtgg
181gcgactcgga cttgcagctg gagcggatca gcgtctacta caacgaggcc tcttctcaca
241agtacgtgcc tcgagccatt ctggtggacc tggaacccgg aaccatggac agtgtccgct
301caggggcctt tggacatctc ttcaggcctg acaatttcat ctttggtcag agtggggccg
361gcaacaactg ggccaagggt cactacacgg agggggcgga gctggtggat tcggtcctgg
421atgtggtgcg gaaggagtgt gaaaactgcg actgcctgca gggcttccag ctgacccact
481cgctgggggg cggcacgggc tccggcatgg gcacgttgct catcagcaag gtgcgtgagg
541agtatcccga ccgcatcatg aacaccttca gcgtcgtgcc ctcacccaag gtgtcagaca
601cggtggtgga gccctacaac gccacgctgt ccatccacca gctggtggag aacacggatg
661agacctactg catcgacaac gaggcgctct acgacatctg cttccgcacc ctcaagctgg
721ccacgcccac ctacggggac ctcaaccacc tggtatcggc caccatgagc ggagtcacca
781cctccttgcg cttcccgggc cagctcaacg ctgacctgcg caagctggcc gtcaacatgg
841tgcccttccc gcgcctgcac ttcttcatgc ccggcttcgc ccccctcaca gcccggggca
901gccagcagta ccgggccctg accgtgcccg agctcaccca gcagatgttc gatgccaaga
961acatgatggc cgcctgcgac ccgcgccacg gccgctacct gacggtggcc accgtgttcc
1021ggggccgcat gtccatgaag gaggtggacg agcagatgct ggCCATCCAG AGCAAGAACA
1081GCAGCTACTT CGTGGAGTGG ATCCCCAACA ACGTGAAGGT GGCCGTGTGT GACATCCCGC
1141CCCGCGGCCT CAAGATGTCC TCCACCTTCA TCGGGAACAG CACGGCCATC CAGGAGCTGT
1201TCAAGCGCAT CTCCGAGCAG TTCACGGCCA TGTTCCGGCG CAAGGCCTTC CTGCACTGGT
1261ACACGGGCGA GGGCATGGAC GAGATGGAGT TCACCGAGGC CGAGAGCAAC ATGAACGACC
1321TGGTGTCCGA GTACCAGCAG TACCAGGACG CCACGGCCGA GGAAGAGGGC GAGATGTACG
1381AAGACGACGA GGAGGAGTCG GAGGCCCAGG GCCCCAAGTA GAACCCAGCT TTCTTGTACA
1441AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1501AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1561AGGACGATAA CTAAGCCACA GTTTAACTCG AACGCGTTAA GTCgacaatc aacctctgga
1621ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)