Construct: ORF TRCN0000489043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020553.1_s317c1
- DNA Barcode:
- TCTGGAAATAATCATTAATACTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SIK3 (23387)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23387 | SIK3 | SIK family kinase 3 | NM_025164.6 | 95.6% | 95.6% | 1_174del |
| 2 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001366686.2 | 92.2% | 92.1% | 1_174del;1808_1951del |
| 3 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271484.3 | 91% | 91% | 1_174del;2618_2619ins180 |
| 4 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271482.4 | 88.7% | 88.6% | 1_174del;1092_1093ins144;1664_1807del |
| 5 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542723.2 | 88.6% | 88.5% | 0_1ins303;1331_1474del |
| 6 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542724.2 | 88.6% | 88.5% | 0_1ins303;1331_1474del |
| 7 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542725.2 | 88.6% | 88.5% | 0_1ins303;1331_1474del |
| 8 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017425.1 | 88.6% | 88.5% | 0_1ins303;1331_1474del |
| 9 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017426.1 | 88.6% | 88.5% | 0_1ins303;1331_1474del |
| 10 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542722.1 | 87.8% | 87.8% | 1_174del;1808_1951del;2762_2763ins180 |
| 11 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017424.1 | 87.4% | 87.4% | 1_174del;1092_1093ins144;2474_2475ins180 |
| 12 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001281748.3 | 87.2% | 87.2% | 0_1ins303;2141_2142ins180 |
| 13 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001281749.3 | 84.3% | 84.2% | (many diffs) |
| 14 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271485.3 | 59.1% | 58.4% | (many diffs) |
| 15 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017427.1 | 56.8% | 56.8% | (many diffs) |
| 16 | human | 23387 | SIK3 | SIK family kinase 3 | XR_001747816.1 | 44.9% | 1_212del;1847_2036del;2287_2288ins1904 | |
| 17 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542726.1 | 44.4% | 41.6% | (many diffs) |
| 18 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001366687.1 | 11.9% | 11.5% | (many diffs) |
| 19 | mouse | 70661 | Sik3 | SIK family kinase 3 | XM_006510590.2 | 84.7% | 87.6% | (many diffs) |
| 20 | mouse | 70661 | Sik3 | SIK family kinase 3 | NM_027498.3 | 81.7% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3861
- ORF length:
- 3789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcccgcc cgtatcggct actacgagat cgaccgcacc atcggcaagg 121 gcaacttcgc ggtggtcaag cgggccacgc acctcgtcac caaggccaag gttgctatca 181 agatcataga taagacccag ctggatgaag aaaacttgaa gaagattttc cgggaagttc 241 aaattatgaa gatgctttgc cacccccata tcatcaggct ctaccaggtt atggagacag 301 aacggatgat ttatctggtg acagaatatg ctagtggagg ggaaatattt gaccacctgg 361 tggcccatgg tagaatggca gaaaaggagg cacgtcggaa gttcaaacag atcgtcacag 421 ctgtctattt ttgtcactgt cggaacattg ttcatcgtga tttaaaagct gaaaatttac 481 ttctggatgc caatctgaat atcaaaatag cagattttgg tttcagtaac ctcttcactc 541 ctgggcagct gctgaagacc tggtgtggca gccctcccta tgctgcacct gaactctttg 601 aaggaaaaga atatgatggg cccaaagtgg acatctggag ccttggagtt gtcctctacg 661 tgcttgtgtg cggtgccctg ccatttgatg gaagcacact gcagaatctg cgggcccgcg 721 tgctgagtgg aaagttccgc atcccatttt ttatgtccac agaatgtgag catttgatcc 781 gccatatgtt ggtgttagat cccaataagc gcctctccat ggagcagatc tgcaagcaca 841 agtggatgaa gctaggggac gccgatccca actttgacag gttaatagct gaatgccaac 901 aactaaagga agaaagacag gtggaccccc tgaatgagga tgtcctcttg gccatggagg 961 acatgggact ggacaaagaa cagacactgc agtcattaag atcagatgcc tatgatcact 1021 atagtgcaat ctacagcctg ctgtgtgatc gacataagag acataaaacc ctgcgtctcg 1081 gagcacttcc tagcatgccc cgagccctgg cctttcaagc accagtcaat atccaggcgg 1141 agcaggcagg tactgctatg aacatcagcg ttccccaggt gcagctgatc aacccagaga 1201 accaaattgt ggagccggat gggacactga atttggacag tgatgagggt gaagagcctt 1261 cccctgaagc attggtgcgc tatttgtcaa tgaggaggca cacagtgggt gtggctgacc 1321 cacgcacgga agttatggaa gatctgcaga agctcctacc tggctttcct ggagtcaacc 1381 cccaggctcc attcctgcag gtggccccta atgtgaactt catgcacaac ctgttgccta 1441 tgcaaaactt gcaaccaacc gggcaacttg agtacaagga gcagtctctc ctacagccgc 1501 ccacgctaca gctgttgaat ggaatgggcc cccttggccg gagggcatca gatggaggag 1561 ccaacatcca actgcatgcc cagcagctgc tgaagcgccc acggggaccc tctccgcttg 1621 tcaccatgac accagcagtg ccagcagtta cccctgtgga cgaggagagc tcagacgggg 1681 agccagacca ggaagctgtg cagagctcta cctacaagga ctccaacact ctgcacctcc 1741 ctacggagcg tttctcccct gtgcgccggt tctcagatgg ggctgcgagc atccaggcct 1801 tcaaagctca cctggaaaaa atgggcaaca acagcagcat caaacagctg cagcaggagt 1861 gtgagcagct gcagaagatg tacggggggc agattgatga aagaaccctg gagaagaccc 1921 agcagcagca tatgttatac cagcaggagc agcaccatca aattctccag caacaaattc 1981 aagactctat ctgtcctcct cagccatctc cacctcttca ggctgcatgt gaaaatcagc 2041 cagccctcct tacccatcag ctccagaggt taaggattca gccttcaagc ccacccccca 2101 accaccccaa caaccatctc ttcaggcagc ccagtaatag tcctcccccc atgagcagtg 2161 ccatgatcca gcctcacggg gctgcatctt cttcccagtt tcaaggctta ccttcccgca 2221 gtgcaatctt tcagcagcaa cctgagaact gttcctctcc tcccaacgtg gcactaacct 2281 gcttgggtat gcagcagcct gctcagtcac agcaggtcac catccaagtc caagagcctg 2341 ttgacatgct cagcaacatg ccaggcacag ctgcaggctc cagtgggcgc ggcatctcca 2401 tcagccccag tgctggtcag atgcagatgc agcaccgtac caacctgatg gccaccctca 2461 gctatgggca ccgtcccttg tccaagcagc tgagtgctga cagtgcagag gctcacagct 2521 tgaacgtgaa tcggttctcc cctgctaact acgaccaggc gcatttacac ccccatctgt 2581 tttcggacca gtcccggggt tcccccagca gctacagccc ttcaacagga gtggggttct 2641 ctccaaccca agccctgaaa gtccctccac ttgaccaatt ccccaccttc cctcccagtg 2701 cacatcagca gccgccacac tataccacgt cggcactaca gcaggccctg ctgtctccca 2761 cgccgccaga ctatacaaga caccagcagg taccccacat ccttcaagga ctgctttctc 2821 cccggcattc gctcaccggc cactcggaca tccggctgcc cccaacagag tttgcacagc 2881 tcattaaaag gcagcagcaa caacggcagc agcagcagca acagcagcaa cagcaagaat 2941 accaggaact gttcaggcac atgaaccaag gggatgcggg gagtctggct cccagccttg 3001 ggggacagag catgacagag cgccaggctt tatcttatca aaatgctgac tcttatcacc 3061 atcacaccag cccccagcat ctgctacaaa tcagggcaca agaatgtgtc tcacaggctt 3121 cctcacccac cccgccccac gggtatgctc accagccggc actgatgcat tcagagagca 3181 tggaggagga ctgctcgtgt gagggggcca aggatggctt ccaagacagt aagagttcaa 3241 gtacattgac caaaggttgc catgacagcc ctctgctctt gagtaccggt ggacctgggg 3301 accctgaatc tttgctagga actgtgagtc atgcccaaga attggggata catccctatg 3361 gtcatcagcc aactgctgca ttcagtaaaa ataaggtgcc cagcagagag cctgtcatag 3421 ggaactgcat ggatagaagt tctccaggac aagcagtgga gctgccggat cacaatgggc 3481 tcgggtaccc agcacgcccc tccgtccatg agcaccacag gccccgggCC CTCCAGAGAC 3541 ACCACACGAT CCAGAACAGC GACGATGCTT ATGTACAGCT GGATAACTTG CCAGGAATGA 3601 GTCTCGTGGC TGGGAAAGCA CTTAGCTCTG CCCGGATGTC GGATGCAGTT CTCAGTCAGT 3661 CTTCGCTCAT GGGCAGCCAG CAGTTTCAGG ATGGGGAAAA TGAGGAATGT GGGGCAAGCC 3721 TGGGAGGTCA TGAGCACCCA GACCTGAGTG ATGGCAGCCA GCATTTAAAC TCCTCTTGCT 3781 ATCCATCTAC GTGTATTACA GACATTCTGC TCAGCTACAA GCACCCCGAA GTCTCCTTCA 3841 GCATGGAGCA GGCAGGCGTG TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 3901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCTGTAAC 3961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTGGAAATAA 4021 TCATTAATAC TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 4081 att