Construct: ORF TRCN0000489043

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020553.1_s317c1
DNA Barcode:
TCTGGAAATAATCATTAATACTGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SIK3 (23387)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489043
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23387 SIK3 SIK family kinase 3 NM_025164.6 95.6% 95.6% 1_174del
2 human 23387 SIK3 SIK family kinase 3 NM_001366686.2 92.2% 92.1% 1_174del;1808_1951del
3 human 23387 SIK3 SIK family kinase 3 XM_005271484.3 91% 91% 1_174del;2618_2619ins180
4 human 23387 SIK3 SIK family kinase 3 XM_005271482.4 88.7% 88.6% 1_174del;1092_1093ins144;1664_1807del
5 human 23387 SIK3 SIK family kinase 3 XM_011542723.2 88.6% 88.5% 0_1ins303;1331_1474del
6 human 23387 SIK3 SIK family kinase 3 XM_011542724.2 88.6% 88.5% 0_1ins303;1331_1474del
7 human 23387 SIK3 SIK family kinase 3 XM_011542725.2 88.6% 88.5% 0_1ins303;1331_1474del
8 human 23387 SIK3 SIK family kinase 3 XM_017017425.1 88.6% 88.5% 0_1ins303;1331_1474del
9 human 23387 SIK3 SIK family kinase 3 XM_017017426.1 88.6% 88.5% 0_1ins303;1331_1474del
10 human 23387 SIK3 SIK family kinase 3 XM_011542722.1 87.8% 87.8% 1_174del;1808_1951del;2762_2763ins180
11 human 23387 SIK3 SIK family kinase 3 XM_017017424.1 87.4% 87.4% 1_174del;1092_1093ins144;2474_2475ins180
12 human 23387 SIK3 SIK family kinase 3 NM_001281748.3 87.2% 87.2% 0_1ins303;2141_2142ins180
13 human 23387 SIK3 SIK family kinase 3 NM_001281749.3 84.3% 84.2% (many diffs)
14 human 23387 SIK3 SIK family kinase 3 XM_005271485.3 59.1% 58.4% (many diffs)
15 human 23387 SIK3 SIK family kinase 3 XM_017017427.1 56.8% 56.8% (many diffs)
16 human 23387 SIK3 SIK family kinase 3 XR_001747816.1 44.9% 1_212del;1847_2036del;2287_2288ins1904
17 human 23387 SIK3 SIK family kinase 3 XM_011542726.1 44.4% 41.6% (many diffs)
18 human 23387 SIK3 SIK family kinase 3 NM_001366687.1 11.9% 11.5% (many diffs)
19 mouse 70661 Sik3 SIK family kinase 3 XM_006510590.2 84.7% 87.6% (many diffs)
20 mouse 70661 Sik3 SIK family kinase 3 NM_027498.3 81.7% 84.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3861
ORF length:
3789
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgcccgcc cgtatcggct actacgagat cgaccgcacc atcggcaagg
121gcaacttcgc ggtggtcaag cgggccacgc acctcgtcac caaggccaag gttgctatca
181agatcataga taagacccag ctggatgaag aaaacttgaa gaagattttc cgggaagttc
241aaattatgaa gatgctttgc cacccccata tcatcaggct ctaccaggtt atggagacag
301aacggatgat ttatctggtg acagaatatg ctagtggagg ggaaatattt gaccacctgg
361tggcccatgg tagaatggca gaaaaggagg cacgtcggaa gttcaaacag atcgtcacag
421ctgtctattt ttgtcactgt cggaacattg ttcatcgtga tttaaaagct gaaaatttac
481ttctggatgc caatctgaat atcaaaatag cagattttgg tttcagtaac ctcttcactc
541ctgggcagct gctgaagacc tggtgtggca gccctcccta tgctgcacct gaactctttg
601aaggaaaaga atatgatggg cccaaagtgg acatctggag ccttggagtt gtcctctacg
661tgcttgtgtg cggtgccctg ccatttgatg gaagcacact gcagaatctg cgggcccgcg
721tgctgagtgg aaagttccgc atcccatttt ttatgtccac agaatgtgag catttgatcc
781gccatatgtt ggtgttagat cccaataagc gcctctccat ggagcagatc tgcaagcaca
841agtggatgaa gctaggggac gccgatccca actttgacag gttaatagct gaatgccaac
901aactaaagga agaaagacag gtggaccccc tgaatgagga tgtcctcttg gccatggagg
961acatgggact ggacaaagaa cagacactgc agtcattaag atcagatgcc tatgatcact
1021atagtgcaat ctacagcctg ctgtgtgatc gacataagag acataaaacc ctgcgtctcg
1081gagcacttcc tagcatgccc cgagccctgg cctttcaagc accagtcaat atccaggcgg
1141agcaggcagg tactgctatg aacatcagcg ttccccaggt gcagctgatc aacccagaga
1201accaaattgt ggagccggat gggacactga atttggacag tgatgagggt gaagagcctt
1261cccctgaagc attggtgcgc tatttgtcaa tgaggaggca cacagtgggt gtggctgacc
1321cacgcacgga agttatggaa gatctgcaga agctcctacc tggctttcct ggagtcaacc
1381cccaggctcc attcctgcag gtggccccta atgtgaactt catgcacaac ctgttgccta
1441tgcaaaactt gcaaccaacc gggcaacttg agtacaagga gcagtctctc ctacagccgc
1501ccacgctaca gctgttgaat ggaatgggcc cccttggccg gagggcatca gatggaggag
1561ccaacatcca actgcatgcc cagcagctgc tgaagcgccc acggggaccc tctccgcttg
1621tcaccatgac accagcagtg ccagcagtta cccctgtgga cgaggagagc tcagacgggg
1681agccagacca ggaagctgtg cagagctcta cctacaagga ctccaacact ctgcacctcc
1741ctacggagcg tttctcccct gtgcgccggt tctcagatgg ggctgcgagc atccaggcct
1801tcaaagctca cctggaaaaa atgggcaaca acagcagcat caaacagctg cagcaggagt
1861gtgagcagct gcagaagatg tacggggggc agattgatga aagaaccctg gagaagaccc
1921agcagcagca tatgttatac cagcaggagc agcaccatca aattctccag caacaaattc
1981aagactctat ctgtcctcct cagccatctc cacctcttca ggctgcatgt gaaaatcagc
2041cagccctcct tacccatcag ctccagaggt taaggattca gccttcaagc ccacccccca
2101accaccccaa caaccatctc ttcaggcagc ccagtaatag tcctcccccc atgagcagtg
2161ccatgatcca gcctcacggg gctgcatctt cttcccagtt tcaaggctta ccttcccgca
2221gtgcaatctt tcagcagcaa cctgagaact gttcctctcc tcccaacgtg gcactaacct
2281gcttgggtat gcagcagcct gctcagtcac agcaggtcac catccaagtc caagagcctg
2341ttgacatgct cagcaacatg ccaggcacag ctgcaggctc cagtgggcgc ggcatctcca
2401tcagccccag tgctggtcag atgcagatgc agcaccgtac caacctgatg gccaccctca
2461gctatgggca ccgtcccttg tccaagcagc tgagtgctga cagtgcagag gctcacagct
2521tgaacgtgaa tcggttctcc cctgctaact acgaccaggc gcatttacac ccccatctgt
2581tttcggacca gtcccggggt tcccccagca gctacagccc ttcaacagga gtggggttct
2641ctccaaccca agccctgaaa gtccctccac ttgaccaatt ccccaccttc cctcccagtg
2701cacatcagca gccgccacac tataccacgt cggcactaca gcaggccctg ctgtctccca
2761cgccgccaga ctatacaaga caccagcagg taccccacat ccttcaagga ctgctttctc
2821cccggcattc gctcaccggc cactcggaca tccggctgcc cccaacagag tttgcacagc
2881tcattaaaag gcagcagcaa caacggcagc agcagcagca acagcagcaa cagcaagaat
2941accaggaact gttcaggcac atgaaccaag gggatgcggg gagtctggct cccagccttg
3001ggggacagag catgacagag cgccaggctt tatcttatca aaatgctgac tcttatcacc
3061atcacaccag cccccagcat ctgctacaaa tcagggcaca agaatgtgtc tcacaggctt
3121cctcacccac cccgccccac gggtatgctc accagccggc actgatgcat tcagagagca
3181tggaggagga ctgctcgtgt gagggggcca aggatggctt ccaagacagt aagagttcaa
3241gtacattgac caaaggttgc catgacagcc ctctgctctt gagtaccggt ggacctgggg
3301accctgaatc tttgctagga actgtgagtc atgcccaaga attggggata catccctatg
3361gtcatcagcc aactgctgca ttcagtaaaa ataaggtgcc cagcagagag cctgtcatag
3421ggaactgcat ggatagaagt tctccaggac aagcagtgga gctgccggat cacaatgggc
3481tcgggtaccc agcacgcccc tccgtccatg agcaccacag gccccgggCC CTCCAGAGAC
3541ACCACACGAT CCAGAACAGC GACGATGCTT ATGTACAGCT GGATAACTTG CCAGGAATGA
3601GTCTCGTGGC TGGGAAAGCA CTTAGCTCTG CCCGGATGTC GGATGCAGTT CTCAGTCAGT
3661CTTCGCTCAT GGGCAGCCAG CAGTTTCAGG ATGGGGAAAA TGAGGAATGT GGGGCAAGCC
3721TGGGAGGTCA TGAGCACCCA GACCTGAGTG ATGGCAGCCA GCATTTAAAC TCCTCTTGCT
3781ATCCATCTAC GTGTATTACA GACATTCTGC TCAGCTACAA GCACCCCGAA GTCTCCTTCA
3841GCATGGAGCA GGCAGGCGTG TAGGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
3901AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCTGTAAC
3961TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTGGAAATAA
4021TCATTAATAC TGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
4081att

Download FASTA (ORF) (Full)

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