Construct: ORF TRCN0000489121
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020290.1_s317c1
- DNA Barcode:
- ACCATGACATTCTAAAGTACAACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CAMK1D (57118)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489121
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_020397.4 | 99.5% | 99.4% | 914A>G;948C>G;1071_1072insTTG |
| 2 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_006717483.4 | 98.1% | 96.3% | (many diffs) |
| 3 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_006717482.3 | 93.5% | 90% | (many diffs) |
| 4 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_153498.4 | 90.9% | 89.6% | (many diffs) |
| 5 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519591.3 | 85.7% | 79.7% | (many diffs) |
| 6 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | NM_001351032.2 | 72.4% | 72.3% | (many diffs) |
| 7 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519592.3 | 72.3% | 70.2% | (many diffs) |
| 8 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519593.3 | 71.8% | 70.3% | (many diffs) |
| 9 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519595.3 | 65.9% | 64.6% | (many diffs) |
| 10 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_017016438.2 | 65.9% | 64.6% | (many diffs) |
| 11 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_024448087.1 | 65.9% | 64.6% | (many diffs) |
| 12 | human | 57118 | CAMK1D | calcium/calmodulin dependen... | XM_011519594.3 | 55.6% | 54.3% | (many diffs) |
| 13 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497470.3 | 89% | 95.2% | (many diffs) |
| 14 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290375.1 | 85.4% | 89.7% | (many diffs) |
| 15 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_177343.4 | 82% | 88.6% | (many diffs) |
| 16 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290374.1 | 79.1% | 82.7% | (many diffs) |
| 17 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_011238973.2 | 77.2% | 80.2% | (many diffs) |
| 18 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | NM_001290376.1 | 76.9% | 78.8% | (many diffs) |
| 19 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497469.3 | 74.2% | 80.6% | (many diffs) |
| 20 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497471.3 | 72.2% | 77.3% | (many diffs) |
| 21 | mouse | 227541 | Camk1d | calcium/calmodulin-dependen... | XM_006497473.3 | 50.2% | 53.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1146
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcccgg gagaacggcg agagcagctc ctcctggaaa aagcaagctg 121 aagacatcaa gaagatcttc gagttcaaag agaccctcgg aaccggggcc ttttccgaag 181 tggttttagc tgaagagaag gcaactggca agctctttgc tgtgaagtgt atccctaaga 241 aggcgctgaa gggcaaggaa agcagcatag agaatgagat agccgtcctg agaaagatta 301 agcatgaaaa tattgttgcc ctggaagaca tttatgaaag cccaaatcac ctgtacttgg 361 tcatgcagct ggtgtccggt ggagagctgt ttgaccggat agtggagaag gggttttata 421 cagagaagga tgccagcact ctgatccgcc aagtcttgga cgccgtgtac tatctccaca 481 gaatgggcat cgtccacaga gacctcaagc ccgaaaatct cttgtactac agtcaagatg 541 aggagtccaa aataatgatc agtgactttg gattgtcaaa aatggagggc aaaggagatg 601 tgatgtccac tgcctgtgga actccaggct atgtcgctcc tgaagtcctc gcccagaaac 661 cttacagcaa agccgttgac tgctggtcca tcggagtgat tgcctacatc ttgctctgcg 721 gctaccctcc tttttatgat gaaaatgact ccaagctctt tgagcagatc ctcaaggcgg 781 aatatgagtt tgactctccc tactgggatg acatctccga ctctgcaaaa gacttcattc 841 ggaaccTGAT GGAGAAGGAC CCGAATAAAA GATACACGTG TGAGCAGGCA GCTCGGCACC 901 CATGGATCGC TGGTGACACA GCCCTCAACA AAAACATCCA CGAGTCCGTC AGCGCCCAGA 961 TCCGGAAAAA CTTTGCCAAG AGCAGATGGA GACAAGCATT TAATGCCACG GCCGTCGTGA 1021 GACATATGAG AAAACTACAC CTCGGCAGCA GCCTGGACAG TTCAAATGCA AGTGTTTCGA 1081 GCAGCCTCAG TTTGGCCAGC CAAAAAGACT GTGCGTATGT AGCAAAACCA GAATCCCTCA 1141 GCTTGTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCATG ACATTCTAAA GTACAACGAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt