Construct: ORF TRCN0000489374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019697.1_s317c1
- DNA Barcode:
- GACATACGTTCGACTGTGGTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK2A (815)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363990.1 | 99.8% | 99.7% | 1377T>C;1434_1435insG |
| 2 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_171825.2 | 99.8% | 99.7% | 1377T>C;1434_1435insG |
| 3 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001369025.2 | 99.7% | 99.5% | 1377T>C;1434_1434delGinsCG |
| 4 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363989.1 | 97.6% | 97.5% | 984_1016del;1410T>C;1467_1468insG |
| 5 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_015981.4 | 97.6% | 97.5% | 984_1016del;1410T>C;1467_1468insG |
| 6 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172083.3 | 80% | 85.2% | (many diffs) |
| 7 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249864.3 | 77.7% | 82.6% | (many diffs) |
| 8 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012665.1 | 77.6% | 82.6% | (many diffs) |
| 9 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172081.3 | 76.2% | 81.1% | (many diffs) |
| 10 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012664.1 | 76.1% | 81.1% | (many diffs) |
| 11 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172084.3 | 75.4% | 80.6% | (many diffs) |
| 12 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715784.2 | 74.3% | 78.9% | (many diffs) |
| 13 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172079.2 | 74.2% | 78.8% | (many diffs) |
| 14 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172080.3 | 74.1% | 78.8% | (many diffs) |
| 15 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249862.3 | 73% | 77.6% | (many diffs) |
| 16 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001293170.2 | 71.1% | 75.5% | (many diffs) |
| 17 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172078.3 | 71.1% | 75.5% | (many diffs) |
| 18 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_177407.4 | 93% | 99.5% | (many diffs) |
| 19 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_017317805.2 | 93% | 99.3% | (many diffs) |
| 20 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_006525541.4 | 90.9% | 97.3% | (many diffs) |
| 21 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514479.2 | 76.1% | 81.3% | (many diffs) |
| 22 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514476.1 | 73.9% | 78.9% | (many diffs) |
| 23 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514477.1 | 73.9% | 78.9% | (many diffs) |
| 24 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514475.1 | 72.9% | 77.8% | (many diffs) |
| 25 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314240.1 | 72.8% | 77.6% | (many diffs) |
| 26 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174054.1 | 72.4% | 77.3% | (many diffs) |
| 27 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_007595.5 | 70.9% | 75.6% | (many diffs) |
| 28 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314239.1 | 70.8% | 75.5% | (many diffs) |
| 29 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174053.1 | 70.3% | 74.9% | (many diffs) |
| 30 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514469.2 | 61.3% | 65.2% | (many diffs) |
| 31 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514470.3 | 61.3% | 65.2% | (many diffs) |
| 32 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514467.2 | 59.9% | 63.7% | (many diffs) |
| 33 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314238.1 | 59.8% | 63.7% | (many diffs) |
| 34 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314237.1 | 59.2% | 62.9% | (many diffs) |
| 35 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514465.2 | 57.9% | 61.6% | (many diffs) |
| 36 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514464.2 | 57.8% | 61.5% | (many diffs) |
| 37 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_001286809.1 | 35.2% | 34.6% | (many diffs) |
| 38 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_009792.3 | 34.5% | 33.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1512
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc accatcacct gcacccgctt cacggaagag taccagctct 121 tcgaggaatt gggcaaggga gccttctcgg tggtgcgaag gtgtgtgaag gtgctggctg 181 gccaggagta tgctgccaag atcatcaaca caaagaagct gtcagccaga gaccatcaga 241 agctggagcg tgaagcccgc atctgccgcc tgctgaagca ccccaacatc gtccgactac 301 atgacagcat ctcagaggag ggacaccact acctgatctt cgacctggtc actggtgggg 361 aactgtttga agatatcgtg gcccgggagt attacagtga ggcggatgcc agtcactgta 421 tccagcagat cctggaggct gtgctgcact gccaccagat gggggtggtg caccgggacc 481 tgaagcctga gaatctgttg ctggcctcca agctcaaggg tgccgcagtg aagctggcag 541 actttggcct ggccatagag gtggaggggg agcagcaggc atggtttggg tttgcaggga 601 ctcctggata tctctcccca gaagtgctgc ggaaggaccc gtacgggaag cctgtggacc 661 tgtgggcttg tggggtcatc ctgtacatcc tgctggttgg gtaccccccg ttctgggatg 721 aggaccagca ccgcctgtac cagcagatca aagccggcgc ctatgatttc ccatcgccgg 781 aatgggacac tgtcaccccg gaagccaagg atctgatcaa taagatgctg accattaacc 841 catccaaacg catcacagct gccgaagccc ttaagcaccc ctggatctcg caccgctcca 901 ccgtggcatc ctgcatgcac agacaggaga ccgtggactg cctgaagaag ttcaatgcca 961 ggaggaaact gaagggagcc attctcacca cgatgctggc caccaggaac ttctccggag 1021 ggaagagtgg gggaaacaag aagagcgatg gtgtgaagga atcctcagag agcaccaaca 1081 ccaccatcga ggatgaagac accaaagtgc ggaaacagga aattataaaa gtgacagagc 1141 agctgattga agccataagc aatggagatt ttgagtccTA CACGAAGATG TGCGACCCTG 1201 GCATGACAGC CTTCGAACCT GAGGCCCTGG GGAACCTGGT TGAGGGCCTG GACTTCCATC 1261 GATTCTATTT TGAAAACCTG TGGTCCCGGA ACAGCAAGCC CGTGCACACC ACCATCCTGA 1321 ATCCCCACAT CCACCTGATG GGCGACGAGT CAGCCTGCAT CGCCTACATC CGCATCACGC 1381 AGTACCTGGA CGCTGGCGGC ATCCCACGCA CCGCCCAGTC GGAGGAGACC CGTGTCTGGC 1441 ACCGCCGGGA CGGCAAATGG CAGATCGTCC ACTTCCACAG ATCTGGGGCG CCCTCCGTCC 1501 TGCCCCACGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACATA CGTTCGACTG TGGTTCTCAC 1681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt