Construct: ORF ccsbBroad304_02248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013738.1_s304c1, BRDN0000399242
- Derived from:
- ccsbBroadEn_02248
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF40 (9810)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02248
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9810 | RNF40 | ring finger protein 40 | NM_001286572.3 | 99.9% | 100% | 2493A>G |
| 2 | human | 9810 | RNF40 | ring finger protein 40 | NM_014771.4 | 99.9% | 100% | 2493A>G |
| 3 | human | 9810 | RNF40 | ring finger protein 40 | NM_001207033.1 | 99.9% | 99.9% | 2725_2726insAGG |
| 4 | human | 9810 | RNF40 | ring finger protein 40 | XM_011545997.2 | 95% | 92.6% | (many diffs) |
| 5 | human | 9810 | RNF40 | ring finger protein 40 | NM_001207034.1 | 90% | 90% | 991_992ins300 |
| 6 | human | 9810 | RNF40 | ring finger protein 40 | XM_024450500.1 | 83.1% | 81.9% | (many diffs) |
| 7 | mouse | 233900 | Rnf40 | ring finger protein 40 | NM_172281.2 | 87.1% | 92.5% | (many diffs) |
| 8 | mouse | 233900 | Rnf40 | ring finger protein 40 | XM_006507750.2 | 86.7% | 92% | (many diffs) |
| 9 | mouse | 233900 | Rnf40 | ring finger protein 40 | XM_006507749.2 | 86.2% | 91.5% | (many diffs) |
| 10 | mouse | 233900 | Rnf40 | ring finger protein 40 | XM_006507751.3 | 58.5% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3069
- ORF length:
- 3003
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgggccaggc aacaaacgcg ccgccggcga cgggggctca gggcccccgg 121 aaaagaagct gagtcgtgag gagaagacca ccacgactct tatcgagccc attcgtcttg 181 gaggcatctc ttccacggag gagatggacc tgaaggtact acagttcaag aacaagaaac 241 tggcagagcg gctggaacaa cggcaggctt gtgaagatga actccgagaa cgaattgaga 301 agttggagaa gcggcaggcc acagatgatg ccacactcct catcgtcaat cgctactggg 361 cccagctgga tgaaactgtg gaagcccttc tccgatgcca tgagagccag ggggagctgt 421 cttcagcgcc tgaggcacct gggacccagg aggggccaac atgtgatggg actcctctcc 481 cagagccggg gacatcagag ctgagagacc ccttgctgat gcagctgcgg ccccctctca 541 gtgagccggc cttggctttt gtggtggcac tgggtgccag cagcagtgag gaggtggagc 601 tggagctgca aggccgaatg gagttctcca aggcagctgt gtctcgtgtg gtagaggcct 661 cagaccgcct acagcgccgg gtggaggaac tctgtcagcg agtgtacagc cgaggggaca 721 gtgagcccct cagtgaggcg gctcaggcac acacccgaga gctgggccgt gagaaccggc 781 gactgcagga cttggccact cagctgcagg agaaacacca ccgcatctca ttggagtact 841 ccgagctcca ggataaagtg acatcggcag agaccaaggt gctggagatg gagacaacag 901 tggaggactt gcagtgggac atcgagaagc tgcggaagcg agagcaaaag ctcaataagc 961 acctggcaga ggccttagag cagcttaact ctggctacta tgtatctggg agctcctcag 1021 gcttccaggg gggccagatc acactcagca tgcagaagtt tgagatgctg aatgcagagt 1081 tagaggaaaa ccaggaactg gccaacagcc gtatggcaga gctggagaaa ctgcaggccg 1141 aacttcaggg ggctgtgcgg accaatgagc gcctcaaggt ggccctgcgg agccttcctg 1201 aggaggtagt gcgggagacg ggggagtacc gcatgctgca ggcccaattc tcactgctct 1261 acaacgagtc tctgcaagtg aagacccagc tagacgaggc tcggggcctg ctgctggcca 1321 caaagaactc ccacctgcga cacatcgagc acatggagag cgacgagctg gggctgcaga 1381 agaagctacg cacagaggtc attcagctgg aggacacgct ggcccaggta cgcaaggagt 1441 atgagatgct gcgcatcgag tttgagcaga atctggcggc caacgagcag gcggggccca 1501 tcaaccgtga gatgcgccac ctgattagta gtcttcaaaa ccacaaccac cagctaaaag 1561 gggacgccca gcgatacaag cggaagcttc gagaagtaca agctgagatt ggcaagctcc 1621 gggcccaggc cagtggctct gcccactcca cccccaacct gggccaccca gaggattctg 1681 gcgtcagtgc cccagcccca gggaaagagg agggtgggcc aggccctgtc agtacccccg 1741 acaacagaaa ggagatggct ccagtgcctg gcaccaccac tactaccact tcagtgaaga 1801 aggaggagct ggtcccctct gaagaggact tccagggtat aacccctggg gcccagggcc 1861 cttcctcccg gggccgagaa cctgaggcca ggcccaagcg ggagcttcgg gaacgggaag 1921 gtcccagcct aggacctcca cctgtagcct ccgctctctc aagggctgat cgggagaagg 1981 ccaaggtgga agaaaccaag cggaaggaat cagaactcct caagggtctc cgagcagagc 2041 tcaagaaggc ccaggagagc cagaaggaga tgaaactgct gctggatatg tacaagtcag 2101 cgcccaagga gcagcgggat aaggtgcagc tcatggcagc ggaacgcaag gctaaggccg 2161 aggttgatga gctgcggagc cgcatccggg aattggagga gagggatcga agggagagca 2221 agaagatcgc ggatgaggat gccctgcggc gcattcggca ggcagaggag cagatagaac 2281 acctgcagcg caagctgggt gccaccaagc aggaggagga ggctctgctc tcagagatgg 2341 atgtgacagg tcaggctttt gaggacatgc aggaacagaa cgggcggctg ctacagcagt 2401 tgcgggaaaa ggatgatgcc aactttaagc taatgtcaga gcggatcaag gccaaccaga 2461 ttcacaagct gctgcgggag gagaaggatg agttgggcga gcaggtcctt ggcctcaagt 2521 cccaggtgga tgcccagctg ctgactgtgc agaagctgga ggagaaggag cgagccttgc 2581 agggcagcct cgggggtgtg gagaaggagc tgacgctgcg cagccaagcc ctggagctca 2641 acaagcggaa ggctgtagaa gccgcccagc tggccgagga cctgaaggtg cagctggagc 2701 acgtgcagac tcggctgcgg gagatccagc cctgcctggc agagagccgg gctgctcgtg 2761 agaaagagag cttcaacctc aagagggctc aggaggacat ctcacggctg cggcgcaagc 2821 tggaaaagca gaggaaggtg gaggtctacg cagatgccga cgaaatcctc caggaggaga 2881 tcaaggagta caaggcgcgg ttgacctgcc cctgctgtaa cacccgcaag aaggatgcag 2941 tccttaccaa gtgcttccac gttttctgct tcgagtgcgt gcggggccgc tatgaggccc 3001 gccagaggaa gtgccccaag tgcaacgcgg cctttggtgc ccacgacttc catcgtatct 3061 acatcagctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 3121 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 3181 tcaacctctg gatta