Construct: ORF ccsbBroad304_02248

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013738.1_s304c1, BRDN0000399242
Derived from:
ccsbBroadEn_02248
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF40 (9810)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02248
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9810 RNF40 ring finger protein 40 NM_001286572.3 99.9% 100% 2493A>G
2 human 9810 RNF40 ring finger protein 40 NM_014771.4 99.9% 100% 2493A>G
3 human 9810 RNF40 ring finger protein 40 NM_001207033.1 99.9% 99.9% 2725_2726insAGG
4 human 9810 RNF40 ring finger protein 40 XM_011545997.2 95% 92.6% (many diffs)
5 human 9810 RNF40 ring finger protein 40 NM_001207034.1 90% 90% 991_992ins300
6 human 9810 RNF40 ring finger protein 40 XM_024450500.1 83.1% 81.9% (many diffs)
7 mouse 233900 Rnf40 ring finger protein 40 NM_172281.2 87.1% 92.5% (many diffs)
8 mouse 233900 Rnf40 ring finger protein 40 XM_006507750.2 86.7% 92% (many diffs)
9 mouse 233900 Rnf40 ring finger protein 40 XM_006507749.2 86.2% 91.5% (many diffs)
10 mouse 233900 Rnf40 ring finger protein 40 XM_006507751.3 58.5% 62.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3069
ORF length:
3003
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tgggccaggc aacaaacgcg ccgccggcga cgggggctca gggcccccgg
121aaaagaagct gagtcgtgag gagaagacca ccacgactct tatcgagccc attcgtcttg
181gaggcatctc ttccacggag gagatggacc tgaaggtact acagttcaag aacaagaaac
241tggcagagcg gctggaacaa cggcaggctt gtgaagatga actccgagaa cgaattgaga
301agttggagaa gcggcaggcc acagatgatg ccacactcct catcgtcaat cgctactggg
361cccagctgga tgaaactgtg gaagcccttc tccgatgcca tgagagccag ggggagctgt
421cttcagcgcc tgaggcacct gggacccagg aggggccaac atgtgatggg actcctctcc
481cagagccggg gacatcagag ctgagagacc ccttgctgat gcagctgcgg ccccctctca
541gtgagccggc cttggctttt gtggtggcac tgggtgccag cagcagtgag gaggtggagc
601tggagctgca aggccgaatg gagttctcca aggcagctgt gtctcgtgtg gtagaggcct
661cagaccgcct acagcgccgg gtggaggaac tctgtcagcg agtgtacagc cgaggggaca
721gtgagcccct cagtgaggcg gctcaggcac acacccgaga gctgggccgt gagaaccggc
781gactgcagga cttggccact cagctgcagg agaaacacca ccgcatctca ttggagtact
841ccgagctcca ggataaagtg acatcggcag agaccaaggt gctggagatg gagacaacag
901tggaggactt gcagtgggac atcgagaagc tgcggaagcg agagcaaaag ctcaataagc
961acctggcaga ggccttagag cagcttaact ctggctacta tgtatctggg agctcctcag
1021gcttccaggg gggccagatc acactcagca tgcagaagtt tgagatgctg aatgcagagt
1081tagaggaaaa ccaggaactg gccaacagcc gtatggcaga gctggagaaa ctgcaggccg
1141aacttcaggg ggctgtgcgg accaatgagc gcctcaaggt ggccctgcgg agccttcctg
1201aggaggtagt gcgggagacg ggggagtacc gcatgctgca ggcccaattc tcactgctct
1261acaacgagtc tctgcaagtg aagacccagc tagacgaggc tcggggcctg ctgctggcca
1321caaagaactc ccacctgcga cacatcgagc acatggagag cgacgagctg gggctgcaga
1381agaagctacg cacagaggtc attcagctgg aggacacgct ggcccaggta cgcaaggagt
1441atgagatgct gcgcatcgag tttgagcaga atctggcggc caacgagcag gcggggccca
1501tcaaccgtga gatgcgccac ctgattagta gtcttcaaaa ccacaaccac cagctaaaag
1561gggacgccca gcgatacaag cggaagcttc gagaagtaca agctgagatt ggcaagctcc
1621gggcccaggc cagtggctct gcccactcca cccccaacct gggccaccca gaggattctg
1681gcgtcagtgc cccagcccca gggaaagagg agggtgggcc aggccctgtc agtacccccg
1741acaacagaaa ggagatggct ccagtgcctg gcaccaccac tactaccact tcagtgaaga
1801aggaggagct ggtcccctct gaagaggact tccagggtat aacccctggg gcccagggcc
1861cttcctcccg gggccgagaa cctgaggcca ggcccaagcg ggagcttcgg gaacgggaag
1921gtcccagcct aggacctcca cctgtagcct ccgctctctc aagggctgat cgggagaagg
1981ccaaggtgga agaaaccaag cggaaggaat cagaactcct caagggtctc cgagcagagc
2041tcaagaaggc ccaggagagc cagaaggaga tgaaactgct gctggatatg tacaagtcag
2101cgcccaagga gcagcgggat aaggtgcagc tcatggcagc ggaacgcaag gctaaggccg
2161aggttgatga gctgcggagc cgcatccggg aattggagga gagggatcga agggagagca
2221agaagatcgc ggatgaggat gccctgcggc gcattcggca ggcagaggag cagatagaac
2281acctgcagcg caagctgggt gccaccaagc aggaggagga ggctctgctc tcagagatgg
2341atgtgacagg tcaggctttt gaggacatgc aggaacagaa cgggcggctg ctacagcagt
2401tgcgggaaaa ggatgatgcc aactttaagc taatgtcaga gcggatcaag gccaaccaga
2461ttcacaagct gctgcgggag gagaaggatg agttgggcga gcaggtcctt ggcctcaagt
2521cccaggtgga tgcccagctg ctgactgtgc agaagctgga ggagaaggag cgagccttgc
2581agggcagcct cgggggtgtg gagaaggagc tgacgctgcg cagccaagcc ctggagctca
2641acaagcggaa ggctgtagaa gccgcccagc tggccgagga cctgaaggtg cagctggagc
2701acgtgcagac tcggctgcgg gagatccagc cctgcctggc agagagccgg gctgctcgtg
2761agaaagagag cttcaacctc aagagggctc aggaggacat ctcacggctg cggcgcaagc
2821tggaaaagca gaggaaggtg gaggtctacg cagatgccga cgaaatcctc caggaggaga
2881tcaaggagta caaggcgcgg ttgacctgcc cctgctgtaa cacccgcaag aaggatgcag
2941tccttaccaa gtgcttccac gttttctgct tcgagtgcgt gcggggccgc tatgaggccc
3001gccagaggaa gtgccccaag tgcaacgcgg cctttggtgc ccacgacttc catcgtatct
3061acatcagctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
3121tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
3181tcaacctctg gatta

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