Construct: ORF ccsbBroad304_05206

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013949.1_s304c1, BRDN0000400067
Derived from:
ccsbBroadEn_05206
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB (203068)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05206
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203068 TUBB tubulin beta class I NM_178014.4 100% 100%
2 human 203068 TUBB tubulin beta class I NM_001293212.2 94.1% 92.6% (many diffs)
3 human 203068 TUBB tubulin beta class I NM_001293214.2 90% 90% 34_35ins132
4 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 84.7% 95.7% (many diffs)
5 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 84.6% 95.2% (many diffs)
6 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 84.4% 97.3% (many diffs)
7 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 83.9% 96.4% (many diffs)
8 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 83.9% 96.4% (many diffs)
9 human 203068 TUBB tubulin beta class I NM_001293215.2 83.7% 83.7% 0_1ins216
10 human 203068 TUBB tubulin beta class I NM_001293216.2 83.7% 83.7% 0_1ins216
11 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 81.8% 91.3% (many diffs)
12 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 81.1% 88.9% (many diffs)
13 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 80.9% 90.3% (many diffs)
14 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 80.9% 90.3% (many diffs)
15 human 260334 TUBB8B tubulin beta 8B NM_001358689.1 80.5% 87.1% (many diffs)
16 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 76.2% 87.5% (many diffs)
17 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 75.6% 82.9% (many diffs)
18 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 75.3% 86.4% (many diffs)
19 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 70.6% 81.3% (many diffs)
20 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 70.6% 81.3% (many diffs)
21 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 70.3% 71.2% (many diffs)
22 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 69% 76.9% (many diffs)
23 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 68.9% 76.8% (many diffs)
24 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 68.7% 75.6% (many diffs)
25 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 68% 75.5% (many diffs)
26 human 260334 TUBB8B tubulin beta 8B XM_024451145.1 67.5% 74.3% (many diffs)
27 human 260334 TUBB8B tubulin beta 8B XM_024451146.1 66.1% 71.6% (many diffs)
28 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 60.6% 67.1% (many diffs)
29 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 60.6% 67.1% (many diffs)
30 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 59.7% 66.2% (many diffs)
31 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 55.7% 61.6% (many diffs)
32 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 55.7% 61.6% (many diffs)
33 human 203068 TUBB tubulin beta class I NM_001293213.2 54.5% 54.5% 362_363ins606
34 human 203068 TUBB tubulin beta class I NR_120608.2 29.1% 1_155del;431_432ins599;889_1916del
35 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 91.4% 100% (many diffs)
36 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 85.1% 95.2% (many diffs)
37 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 84.5% 95.7% (many diffs)
38 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 84.4% 96.4% (many diffs)
39 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 83.1% 97.3% (many diffs)
40 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 82.9% 91.1% (many diffs)
41 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 81.5% 91% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1398
ORF length:
1332
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag ggaaatcgtg cacatccagg ctggtcagtg tggcaaccag atcggtgcca
121agttctggga ggtgatcagt gatgaacatg gcatcgaccc caccggcacc taccacgggg
181acagcgacct gcagctggac cgcatctctg tgtactacaa tgaagccaca ggtggcaaat
241atgttcctcg tgccatcctg gtggatctag aacctgggac catggactct gttcgctcag
301gtccttttgg ccagatcttt agaccagaca actttgtatt tggtcagtct ggggcaggta
361acaactgggc caaaggccac tacacagagg gcgccgagct ggttgattct gtcctggatg
421tggtacggaa ggaggcagag agctgtgact gcctgcaggg cttccagctg acccactcac
481tgggcggggg cacaggctct ggaatgggca ctctccttat cagcaagatc cgagaagaat
541accctgatcg catcatgaat accttcagtg tggtgccttc acccaaagtg tctgacaccg
601tggtcgagcc ctacaatgcc accctctccg tccatcagtt ggtagagaat actgatgaga
661cctattgcat tgacaacgag gccctctatg atatctgctt ccgcactctg aagctgacca
721caccaaccta cggggatctg aaccaccttg tctcagccac catgagtggt gtcaccacct
781gcctccgttt ccctggccag ctcaatgctg acctccgcaa gttggcagtc aacatggtcc
841ccttcccacg tctccatttc tttatgcctg gctttgcccc tctcaccagc cgtggaagcc
901agcagtatcg agctctcaca gtgccggaac tcacccagca ggtcttcgat gccaagaaca
961tgatggctgc ctgtgacccc cgccacggcc gatacctcac cgtggctgct gtcttccgtg
1021gtcggatgtc catgaaggag gtcgatgagc agatgcttaa cgtgcagaac aagaacagca
1081gctactttgt ggaatggatc cccaacaatg tcaagacagc cgtctgtgac atcccacctc
1141gtggcctcaa gatggcagtc accttcattg gcaatagcac agccatccag gagctcttca
1201agcgcatctc ggagcagttc actgccatgt tccgccggaa ggccttcctc cactggtaca
1261caggcgaggg catggacgag atggagttca ccgaggctga gagcaacatg aacgacctcg
1321tctctgagta tcagcagtac caggatgcca ccgcagaaga ggaggaggat ttcggtgagg
1381aggccgaaga ggaggcctac ccaactttct tgtacaaagt ggttggtaag cctatcccta
1441accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1501agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)