Construct: ORF ccsbBroadEn_07108
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009176.1_s300c1, BRDN0000385310
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA4A (7277)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 99.9% | 100% | 1344A>G |
| 2 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 96.5% | 96.6% | 0_1ins45;1299A>G |
| 3 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 85.8% | 96.2% | (many diffs) |
| 4 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 85.3% | 95.9% | (many diffs) |
| 5 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 84.5% | 95.7% | (many diffs) |
| 6 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 84.5% | 95.7% | (many diffs) |
| 7 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 84.1% | 94.2% | (many diffs) |
| 8 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 83.8% | 94.2% | (many diffs) |
| 9 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 83.3% | 92.8% | (many diffs) |
| 10 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 81.7% | 89.7% | (many diffs) |
| 11 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 78.6% | 88.6% | (many diffs) |
| 12 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 78.6% | 88.6% | (many diffs) |
| 13 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 78.6% | 88.6% | (many diffs) |
| 14 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 77.8% | 88.4% | (many diffs) |
| 15 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 73.8% | 82.8% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 70.9% | 78.6% | (many diffs) |
| 17 | human | 7277 | TUBA4A | tubulin alpha 4a | XM_017004824.1 | 65.7% | 65.8% | 0_1ins459;885A>G |
| 18 | human | 80086 | TUBA4B | tubulin alpha 4b | NM_001355221.1 | 49.4% | 34.2% | (many diffs) |
| 19 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 92.6% | 100% | (many diffs) |
| 20 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 89.5% | 96.6% | (many diffs) |
| 21 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 86.4% | 96.2% | (many diffs) |
| 22 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 86.4% | 96.2% | (many diffs) |
| 23 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 85.6% | 95.7% | (many diffs) |
| 24 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 83.7% | 94.2% | (many diffs) |
| 25 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 83.1% | 94.2% | (many diffs) |
| 26 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 80.5% | 89.9% | (many diffs) |
| 27 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 78.8% | 87.6% | (many diffs) |
| 28 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313724.1 | 60.9% | 65.8% | (many diffs) |
| 29 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 50.8% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCG TGAATGCATC TCAGTCCACG TGGGGCAGGC AGGTGTCCAG ATGGGCAATG 121 CCTGCTGGGA GCTCTATTGC TTGGAACATG GGATTCAGCC TGATGGGCAG ATGCCCAGTG 181 ACAAGACCAT TGGTGGAGGG GACGACTCCT TCACCACCTT CTTCTGTGAA ACTGGTGCTG 241 GAAAACACGT ACCCCGGGCA GTTTTTGTGG ATCTGGAGCC TACGGTCATT GATGAGATCC 301 GAAATGGCCC ATACCGACAG CTCTTCCACC CAGAGCAGCT CATCACTGGG AAAGAGGATG 361 CTGCCAACAA CTATGCCCGT GGTCACTATA CCATTGGCAA GGAGATCATT GACCCAGTGC 421 TGGATCGGAT CCGCAAGCTG TCTGACCAGT GCACAGGACT TCAGGGCTTC CTGGTGTTCC 481 ACAGCTTTGG TGGGGGCACT GGCTCTGGCT TCACCTCACT CCTGATGGAG CGGCTCTCTG 541 TTGACTATGG CAAGAAATCC AAGCTGGAAT TCTCCATCTA CCCAGCCCCC CAGGTGTCTA 601 CAGCCGTGGT CGAGCCCTAC AACTCTATCC TGACCACCCA CACCACCCTG GAGCACTCAG 661 ACTGTGCCTT CATGGTGGAC AACGAAGCAA TCTATGACAT CTGCCGCCGC AACCTAGACA 721 TCGAGCGCCC AACCTACACC AACCTCAATC GCCTCATTAG CCAAATTGTC TCCTCCATCA 781 CAGCTTCTCT GCGCTTTGAC GGGGCCCTCA ATGTGGACCT GACAGAGTTC CAGACCAACC 841 TGGTGCCCTA CCCTCGCATC CACTTCCCCC TGGCCACCTA TGCACCAGTC ATCTCTGCAG 901 AAAAGGCATA CCACGAGCAG CTGTCGGTGG CAGAGATCAC CAATGCCTGC TTTGAGCCTG 961 CCAACCAGAT GGTAAAGTGT GATCCCCGGC ACGGCAAGTA CATGGCCTGC TGCCTGCTGT 1021 ACCGTGGAGA TGTGGTGCCC AAGGATGTCA ACGCTGCCAT TGCCGCCATC AAGACCAAGC 1081 GCAGCATTCA GTTTGTGGAC TGGTGCCCCA CAGGCTTCAA GGTTGGTATC AACTACCAGC 1141 CTCCCACTGT GGTGCCTGGG GGTGACCTGG CCAAGGTGCA GCGTGCCGTG TGCATGCTGA 1201 GCAACACGAC CGCCATCGCC GAGGCCTGGG CCCGCCTGGA CCACAAGTTC GACCTGATGT 1261 ATGCCAAGAG GGCGTTTGTG CACTGGTATG TGGGTGAGGG CATGGAGGAG GGTGAGTTCT 1321 CCGAGGCCCG TGAGGATATG GCTGCCCTGG AGAAGGATTA TGAGGAGGTG GGCATCGACT 1381 CCTATGAGGA CGAGGATGAG GGAGAAGAGT ACCCAACTTT CTTGTACAAA GTtggcatta 1441 taagaaagca ttgcttatca atttgttgca acgaac