Construct: sgRNA BRDN0001145269

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTAAGGCGAGAAAAATTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80249

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 68887048 - ABCA8 NNNAAGGGGAGAAAAAATAG NGG 2 0.3429 Tier I
2 NC_000001.11 1 42290185 + FOXJ3 NNNAAGAAGAGAAAAATTAG NGG 2 0.65 Tier II
3 NC_000001.11 1 715900 + OR4F16 NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier II
4 NC_000007.14 7 11663604 + THSD7A NNNAAGGAGAGGAAAATTAG NGG 2 0.4694 Tier II
5 NC_000006.12 6 116178920 - COL10A1 NNNAAGGAAAGAAAAATTAG NGG 2 0.4179 Tier II
6 NC_000006.12 6 116178920 - NT5DC1 NNNAAGGAAAGAAAAATTAG NGG 2 0.4179 Tier II
7 NC_000010.11 10 45628278 + ZFAND4 NNNGAGGCAAGAAAAATTAG NGG 2 0.4018 Tier II
8 NC_000015.10 15 42981102 - UBR1 NNNAAGGAGGGAAAAATTAG NGG 2 0.3611 Tier II
9 NC_000007.14 7 24734865 + GSDME NNNAAGGAGAGAAAAAATAG NGG 2 0.3467 Tier II
10 NC_000005.10 5 95468789 + SKIC3 NNNAAGGCGATAAAAATTAT NGG 2 0.2692 Tier II
11 NC_000005.10 5 142597163 + FGF1 NNNTTGGCGAGAAAAATTAG NGG 2 0.2314 Tier II
12 NC_000006.12 6 13980272 + RNF182 NNNCAGGGGAGAAAAATTAG NGG 2 0.2269 Tier II
13 NC_000010.11 10 45455257 + MARCHF8 NNNAAGGGGAGAAACATTAG NGG 2 0.1753 Tier II
14 NC_000003.12 3 101460271 + SENP7 NNNAAGGAGAGCAAAATTAG NGG 2 0.1711 Tier II
15 NC_000005.10 5 53043846 + ITGA2 NNNAAGGAGAGAAAAATTAG NAG 2 0.1685 Tier II
16 NC_000013.11 13 35474883 - MAB21L1 NNNAAGGGGAGAAAAAGTAG NGG 2 0.0756 Tier II
17 NC_000013.11 13 35474883 - NBEA NNNAAGGGGAGAAAAAGTAG NGG 2 0.0756 Tier II
18 NC_000003.12 3 62329488 + CEP15 NNNAAGGAGAGAAAAATTAG NGA 2 0.0451 Tier II
19 NC_000007.14 7 55379325 + LANCL2 NNNAAGGGGAGAAAAATTAG NGA 2 0.0446 Tier II
20 NC_000003.12 3 158290201 + RSRC1 NNNAAGGCGAGAAAAATTTG NGA 2 0.0374 Tier II
21 NC_000006.12 6 22055956 - CASC15 NNNAAGAAGAGAAAAATTAG NGG 2 0.65 Tier III
22 NC_000002.12 2 113566091 - PGM5P4 NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier III
23 NC_000002.12 2 242126659 - LINC01881 NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier III
24 NC_000020.11 20 64299194 - SEPTIN14P20 NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier III
25 NC_000024.10 Y 24287369 - LINC00266-2P NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier III
26 NC_000024.10 Y 25382779 + SEPTIN14P23 NNNAAGGTAAGAAAAATTAG NGG 2 0.5625 Tier III
27 NC_000017.11 17 49258654 - FLJ40194 NNNAAGGAGAGAAAAATAAG NGG 2 0.4333 Tier III
28 NC_000001.11 1 124922 + LOC124900384 NNNAAGGAAAGAAAAATTAG NGG 2 0.4179 Tier III
29 NC_000006.12 6 114441284 - LOC107986638 NNNAAGGAAAGAAAAATTAG NGG 2 0.4179 Tier III
30 NC_000008.11 8 127333616 + CASC8 NNNAAGGGGAGAAAAATGAG NGG 2 0.2143 Tier III
31 NC_000008.11 8 127333616 + CASC21 NNNAAGGGGAGAAAAATGAG NGG 2 0.2143 Tier III
32 NC_000016.10 16 65311070 - LINC00922 NNNAAGGAGAGAACAATTAG NGG 2 0.1393 Tier III
33 NC_000016.10 16 65311070 - LOC124903780 NNNAAGGAGAGAACAATTAG NGG 2 0.1393 Tier III
34 NC_000012.12 12 127505670 - LOC105370067 NNNAAGGAGAGAAAAGTTAG NGG 2 0.125 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 110053047 - Abca8a NNNAAGGGGAGAAAAAATAG NGG 2 0.3429 Tier I
2 NC_000068.7 2 92949540 + Syt13 NNNAAGAGGAGAAAAATTAG NGG 2 0.6429 Tier II
3 NC_000069.6 3 145389838 + Col24a1 NNNAAGGAGAGAAAAATTAA NGG 2 0.6094 Tier II
4 NC_000082.6 16 45026912 + Cd200r3 NNNAAGGTGAGAAAAATAAG NGG 2 0.5833 Tier II
5 NC_000084.6 18 37799890 + Pcdhgb1 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
6 NC_000084.6 18 37799890 + Pcdhgb2 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
7 NC_000084.6 18 37799890 + Pcdhgb4 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
8 NC_000084.6 18 37799890 + Pcdhgb5 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
9 NC_000084.6 18 37799890 + Pcdhgb6 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
10 NC_000084.6 18 37799890 + Pcdhgb7 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
11 NC_000084.6 18 37799890 + Pcdhga1 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
12 NC_000084.6 18 37799890 + Pcdhga2 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
13 NC_000084.6 18 37799890 + Pcdhga3 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
14 NC_000084.6 18 37799890 + Pcdhga4 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
15 NC_000084.6 18 37799890 + Pcdhga5 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
16 NC_000084.6 18 37799890 + Pcdhga6 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
17 NC_000084.6 18 37799890 + Pcdhga7 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
18 NC_000084.6 18 37799890 + Pcdhga8 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
19 NC_000084.6 18 37799890 + Pcdhga9 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
20 NC_000084.6 18 37799890 + Pcdhga10 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
21 NC_000084.6 18 37799890 + Pcdhga11 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
22 NC_000084.6 18 37799890 + Pcdhga12 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
23 NC_000084.6 18 37799890 + Gm37013 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
24 NC_000084.6 18 37799890 + Gm38666 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
25 NC_000084.6 18 37799890 + Gm38667 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier II
26 NC_000083.6 17 86021326 - Srbd1 NNNAAGGATAGAAAAATTAG NGG 2 0.3467 Tier II
27 NC_000081.6 15 96401127 - Arid2 NNNAATGCGAGAAAAATCAG NGG 2 0.2857 Tier II
28 NC_000078.6 12 100438911 + Ttc7b NNNAAGGCTATAAAAATTAG NGG 2 0.2051 Tier II
29 NC_000072.6 6 35179661 + Nup205 NNNAAGGCTAGAAAAATGAG NGG 2 0.1778 Tier II
30 NC_000071.6 5 73646018 - Sgcb NNNAAGGGGAGAAAAATTAG NAG 2 0.1667 Tier II
31 NC_000068.7 2 161629827 + Ptprt NNNAAGGCTAGAAAAATTAG NGA 2 0.037 Tier II
32 NC_000075.6 9 77975763 - Elovl5 NNNAAGGGGAGAAAAATTAG NTG 2 0.025 Tier II
33 NC_000075.6 9 66009373 - Csnk1g1 NNNAAGGAGAGAAAAATTAG NGC 2 0.0144 Tier II
34 NC_000070.6 4 76265095 - Ptprd NNNAAGGCTAGAAAACTTAG NGG 2 0.0 Tier II
35 NC_000069.6 3 145389838 + Gm35133 NNNAAGGAGAGAAAAATTAA NGG 2 0.6094 Tier III
36 NC_000084.6 18 37799890 + Pcdhgb8 NNNTAGGTGAGAAAAATTAG NGG 2 0.5568 Tier III
37 NC_000077.6 11 44015374 - Gm12153 NNNAAGACTAGAAAAATTAG NGG 2 0.5333 Tier III
38 NC_000071.6 5 87961492 + 1700066N21Rik NNNGAGGAGAGAAAAATTAG NGG 2 0.4063 Tier III
39 NC_000071.6 5 87961492 + BC051076 NNNGAGGAGAGAAAAATTAG NGG 2 0.4063 Tier III
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Other clones with same target sequence:

(none)