Construct: ORF TRCN0000469567

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003644.1_s317c1
Derived from:
ccsbBroadEn_13553
DNA Barcode:
TTGGATCTCAGGTTGACTCAGATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FRYL (285527)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469567
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285527 FRYL FRY like transcription coac... XM_005248093.5 19.7% 19.7% 1_4905del;5556_5573del
2 human 285527 FRYL FRY like transcription coac... XM_011513684.3 19.7% 19.7% 1_4905del;5556_5573del
3 human 285527 FRYL FRY like transcription coac... NM_015030.2 13.3% 13.3% 1_7812del;8463_8480del
4 human 285527 FRYL FRY like transcription coac... XM_017008046.2 13.3% 13.3% 1_7812del;8463_8480del
5 human 285527 FRYL FRY like transcription coac... XM_024453993.1 13.3% 13.3% 1_7812del;8463_8480del
6 human 285527 FRYL FRY like transcription coac... XM_024453994.1 13.3% 13.3% 1_7812del;8463_8480del
7 human 285527 FRYL FRY like transcription coac... XM_005248090.5 13.3% 13.3% 1_7857del;8508_8525del
8 human 285527 FRYL FRY like transcription coac... XM_011513683.3 13.3% 13.3% 1_7857del;8508_8525del
9 human 285527 FRYL FRY like transcription coac... XM_024453990.1 13.3% 13.3% 1_7857del;8508_8525del
10 human 285527 FRYL FRY like transcription coac... XM_024453991.1 13.3% 13.3% 1_7857del;8508_8525del
11 human 285527 FRYL FRY like transcription coac... XM_024453992.1 13.3% 13.3% 1_7857del;8508_8525del
12 human 285527 FRYL FRY like transcription coac... XM_011513682.3 13% 13% 1_8088del
13 human 285527 FRYL FRY like transcription coac... XM_024453989.1 12.9% 12.9% 1_8088del;8739_8756del
14 human 285527 FRYL FRY like transcription coac... XM_011513681.3 12.9% 12.9% 1_8133del
15 human 285527 FRYL FRY like transcription coac... XM_011513680.3 12.9% 12.9% 1_8130del;8781_8798del
16 human 285527 FRYL FRY like transcription coac... XM_024453988.1 12.9% 12.9% 1_8133del;8784_8801del
17 mouse 72313 Fryl FRY like transcription coac... NM_028194.2 11.2% 12.2% (many diffs)
18 mouse 72313 Fryl FRY like transcription coac... XM_006504150.3 11.2% 12.2% (many diffs)
19 mouse 72313 Fryl FRY like transcription coac... XM_006504148.3 11.1% 12.1% (many diffs)
20 mouse 72313 Fryl FRY like transcription coac... XM_006504149.3 11.1% 12.1% (many diffs)
21 mouse 72313 Fryl FRY like transcription coac... XM_011240773.2 11.1% 12.1% (many diffs)
22 mouse 72313 Fryl FRY like transcription coac... XM_006504146.3 10.9% 11.9% (many diffs)
23 mouse 72313 Fryl FRY like transcription coac... XM_017321111.1 10.9% 11.8% (many diffs)
24 mouse 72313 Fryl FRY like transcription coac... XM_006504152.3 10.9% 11.8% (many diffs)
25 mouse 72313 Fryl FRY like transcription coac... XM_006504145.3 10.9% 11.8% (many diffs)
26 mouse 72313 Fryl FRY like transcription coac... XM_006504144.3 10.8% 11.8% (many diffs)
27 mouse 72313 Fryl FRY like transcription coac... XM_006504143.3 10.8% 11.8% (many diffs)
28 mouse 72313 Fryl FRY like transcription coac... XM_006504141.3 10.8% 11.7% (many diffs)
29 mouse 72313 Fryl FRY like transcription coac... XM_017321110.1 10.8% 11.7% (many diffs)
30 mouse 72313 Fryl FRY like transcription coac... XM_006504140.3 10.8% 11.7% (many diffs)
31 mouse 72313 Fryl FRY like transcription coac... XM_006504142.3 10.8% 11.7% (many diffs)
32 mouse 72313 Fryl FRY like transcription coac... XM_006504138.3 10.8% 11.7% (many diffs)
33 mouse 72313 Fryl FRY like transcription coac... XM_006504139.3 10.8% 11.7% (many diffs)
34 mouse 72313 Fryl FRY like transcription coac... XM_006504137.3 10.8% 11.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1275
ORF length:
1209
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc agagcctcta gctcctgaaa gttaccccga gtcagtctgt gaagaggatg
121ttaccttagc tctgaaagag ctagatgaaa gatgtgaaga agaagaagcg gatttctccg
181gactgtctag tcaagatgaa gaagagcaag atggttttcc agaagtacag acgtcgcctc
241tgccgtcacc atttctttct gccatcatag ccgcctttca gcccgtggca tatgatgatg
301aagaggaagc ctggcgctgc cacgtcaatc agatgctgtc tgacaccgac gggtcctctg
361cagtgtttac ttttcatgtg ttttctaggc tgtttcagac aattcaaaga aagtttggag
421aaataactaa tgaggcagtc agctttcttg gtgatagtct gcaacgcatt ggtaccaaat
481ttaaaagttc cttggaagtg atgatgctgt gttcagaatg cccaacagtc tttgtggatg
541ctgaaacact gatgtcatgt ggtttgctgg aaacactcaa gtttggtgtt ttggagttgc
601aagaacacct ggatacatac aatgtgaaaa gagaagccgc tgagcagtgg ctagatgatt
661gtaagaggac atttggtgcc aaagaagaca tgtataggat aaacacagat gcacaagaat
721tggagctctg ccgaagatta tacaaattgc attttcaatt gctgcttctg ttccaggccT
781ACTGTAAACT TATCAACCAA GTAAATACGA TAAAAAATGA AGCAGAGGTC ATCAACATGT
841CAGAGGAACT TGCCCAACTG GAAAGTATCC TCAAAGAAGC TGAGTCCGCT TCCGAAAACG
901AAGAAATTGA CATTTCCAAA GCTGCACAAA CTACTATAGA AACTGCCATT CATTCTTTAA
961TTGAAACTTT GAAAAATAAA GAATTTATAT CAGCTGTAGC ACAAGTCAAA GCTTTCAGAT
1021CTCTCTGGCC CAGTGATATC TTTGGCAGTT GTGAAGATGA CCCTGTACAG ACACTGTTAC
1081ATATATATTT CCATCATCAG ACGCTGGGCC AGACAGGAAG CTTTGCAGTT ATAGGCTCTA
1141ACCTGGACAT GTCAGAAGCC AACTACAAAC TGATGGAACT TAATCTGGAA ATAAGAGAGT
1201CTCTACGCAT GGTGCAATCA TACCAACTTC TAGCACAGGC CAAACCAATG GGAAATATGG
1261TGAGCACTGG ATTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1321CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1381TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTGGATCTCA GGTTGACTCA
1441GATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)