Construct: ORF TRCN0000469567
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003644.1_s317c1
- Derived from:
- ccsbBroadEn_13553
- DNA Barcode:
- TTGGATCTCAGGTTGACTCAGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRYL (285527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469567
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 285527 | FRYL | FRY like transcription coac... | XM_005248093.5 | 19.7% | 19.7% | 1_4905del;5556_5573del |
2 | human | 285527 | FRYL | FRY like transcription coac... | XM_011513684.3 | 19.7% | 19.7% | 1_4905del;5556_5573del |
3 | human | 285527 | FRYL | FRY like transcription coac... | NM_015030.2 | 13.3% | 13.3% | 1_7812del;8463_8480del |
4 | human | 285527 | FRYL | FRY like transcription coac... | XM_017008046.2 | 13.3% | 13.3% | 1_7812del;8463_8480del |
5 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453993.1 | 13.3% | 13.3% | 1_7812del;8463_8480del |
6 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453994.1 | 13.3% | 13.3% | 1_7812del;8463_8480del |
7 | human | 285527 | FRYL | FRY like transcription coac... | XM_005248090.5 | 13.3% | 13.3% | 1_7857del;8508_8525del |
8 | human | 285527 | FRYL | FRY like transcription coac... | XM_011513683.3 | 13.3% | 13.3% | 1_7857del;8508_8525del |
9 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453990.1 | 13.3% | 13.3% | 1_7857del;8508_8525del |
10 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453991.1 | 13.3% | 13.3% | 1_7857del;8508_8525del |
11 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453992.1 | 13.3% | 13.3% | 1_7857del;8508_8525del |
12 | human | 285527 | FRYL | FRY like transcription coac... | XM_011513682.3 | 13% | 13% | 1_8088del |
13 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453989.1 | 12.9% | 12.9% | 1_8088del;8739_8756del |
14 | human | 285527 | FRYL | FRY like transcription coac... | XM_011513681.3 | 12.9% | 12.9% | 1_8133del |
15 | human | 285527 | FRYL | FRY like transcription coac... | XM_011513680.3 | 12.9% | 12.9% | 1_8130del;8781_8798del |
16 | human | 285527 | FRYL | FRY like transcription coac... | XM_024453988.1 | 12.9% | 12.9% | 1_8133del;8784_8801del |
17 | mouse | 72313 | Fryl | FRY like transcription coac... | NM_028194.2 | 11.2% | 12.2% | (many diffs) |
18 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504150.3 | 11.2% | 12.2% | (many diffs) |
19 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504148.3 | 11.1% | 12.1% | (many diffs) |
20 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504149.3 | 11.1% | 12.1% | (many diffs) |
21 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_011240773.2 | 11.1% | 12.1% | (many diffs) |
22 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504146.3 | 10.9% | 11.9% | (many diffs) |
23 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_017321111.1 | 10.9% | 11.8% | (many diffs) |
24 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504152.3 | 10.9% | 11.8% | (many diffs) |
25 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504145.3 | 10.9% | 11.8% | (many diffs) |
26 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504144.3 | 10.8% | 11.8% | (many diffs) |
27 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504143.3 | 10.8% | 11.8% | (many diffs) |
28 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504141.3 | 10.8% | 11.7% | (many diffs) |
29 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_017321110.1 | 10.8% | 11.7% | (many diffs) |
30 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504140.3 | 10.8% | 11.7% | (many diffs) |
31 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504142.3 | 10.8% | 11.7% | (many diffs) |
32 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504138.3 | 10.8% | 11.7% | (many diffs) |
33 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504139.3 | 10.8% | 11.7% | (many diffs) |
34 | mouse | 72313 | Fryl | FRY like transcription coac... | XM_006504137.3 | 10.8% | 11.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1275
- ORF length:
- 1209
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agagcctcta gctcctgaaa gttaccccga gtcagtctgt gaagaggatg 121 ttaccttagc tctgaaagag ctagatgaaa gatgtgaaga agaagaagcg gatttctccg 181 gactgtctag tcaagatgaa gaagagcaag atggttttcc agaagtacag acgtcgcctc 241 tgccgtcacc atttctttct gccatcatag ccgcctttca gcccgtggca tatgatgatg 301 aagaggaagc ctggcgctgc cacgtcaatc agatgctgtc tgacaccgac gggtcctctg 361 cagtgtttac ttttcatgtg ttttctaggc tgtttcagac aattcaaaga aagtttggag 421 aaataactaa tgaggcagtc agctttcttg gtgatagtct gcaacgcatt ggtaccaaat 481 ttaaaagttc cttggaagtg atgatgctgt gttcagaatg cccaacagtc tttgtggatg 541 ctgaaacact gatgtcatgt ggtttgctgg aaacactcaa gtttggtgtt ttggagttgc 601 aagaacacct ggatacatac aatgtgaaaa gagaagccgc tgagcagtgg ctagatgatt 661 gtaagaggac atttggtgcc aaagaagaca tgtataggat aaacacagat gcacaagaat 721 tggagctctg ccgaagatta tacaaattgc attttcaatt gctgcttctg ttccaggccT 781 ACTGTAAACT TATCAACCAA GTAAATACGA TAAAAAATGA AGCAGAGGTC ATCAACATGT 841 CAGAGGAACT TGCCCAACTG GAAAGTATCC TCAAAGAAGC TGAGTCCGCT TCCGAAAACG 901 AAGAAATTGA CATTTCCAAA GCTGCACAAA CTACTATAGA AACTGCCATT CATTCTTTAA 961 TTGAAACTTT GAAAAATAAA GAATTTATAT CAGCTGTAGC ACAAGTCAAA GCTTTCAGAT 1021 CTCTCTGGCC CAGTGATATC TTTGGCAGTT GTGAAGATGA CCCTGTACAG ACACTGTTAC 1081 ATATATATTT CCATCATCAG ACGCTGGGCC AGACAGGAAG CTTTGCAGTT ATAGGCTCTA 1141 ACCTGGACAT GTCAGAAGCC AACTACAAAC TGATGGAACT TAATCTGGAA ATAAGAGAGT 1201 CTCTACGCAT GGTGCAATCA TACCAACTTC TAGCACAGGC CAAACCAATG GGAAATATGG 1261 TGAGCACTGG ATTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1321 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1381 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTGGATCTCA GGTTGACTCA 1441 GATTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt