Construct: ORF TRCN0000475115
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012881.1_s317c1
- Derived from:
- ccsbBroadEn_03477
- DNA Barcode:
- ACTTGGTCCAGACCCATGATCACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKAL1 (54901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475115
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | NM_017774.3 | 100% | 100% | |
| 2 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514718.1 | 96.3% | 96.3% | 1235_1236ins63 |
| 3 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_024446481.1 | 84.1% | 76.9% | (many diffs) |
| 4 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_011514719.2 | 75.7% | 75.1% | (many diffs) |
| 5 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_017010986.1 | 75% | 74.9% | 1302_1303insGGTGAAAG;1305_1305delCins425 |
| 6 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XM_017010987.1 | 50.6% | 50.6% | 0_1ins858 |
| 7 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743500.1 | 47.9% | 1_167del;907_908ins167;1738_3105del | |
| 8 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926267.2 | 46.3% | (many diffs) | |
| 9 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743501.1 | 46% | (many diffs) | |
| 10 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926265.2 | 45.8% | 1_167del;1549_3350del;3707_3785del | |
| 11 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743495.2 | 45.8% | 1_172del;1554_3355del;3712_3790del | |
| 12 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_926266.2 | 44.5% | 1_280del;1662_3463del;3820_3898del | |
| 13 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743496.2 | 41.5% | 1_567del;1949_3750del;4107_4185del | |
| 14 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743499.2 | 31.3% | (many diffs) | |
| 15 | human | 54901 | CDKAL1 | CDK5 regulatory subunit ass... | XR_001743498.2 | 28.1% | (many diffs) | |
| 16 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NM_144536.3 | 85.5% | 90.1% | (many diffs) |
| 17 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_011244342.2 | 66.2% | 71.8% | (many diffs) |
| 18 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_006516762.3 | 49.1% | 49.8% | (many diffs) |
| 19 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | NR_131896.1 | 43.6% | (many diffs) | |
| 20 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_006516763.3 | 42.4% | 43.2% | (many diffs) |
| 21 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_006516764.3 | 42.4% | 43.2% | (many diffs) |
| 22 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_017315589.1 | 42.4% | 43.2% | (many diffs) |
| 23 | mouse | 68916 | Cdkal1 | CDK5 regulatory subunit ass... | XM_017315590.1 | 42.4% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1806
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccttctgca tcctgtgata cactactgga tgacatcgaa gatatcgtgt 121 ctcaggaaga ttcaaaacca caagataggc attttgtaag aaaggatgtt gtcccgaagg 181 tacgaaggcg aaatacccaa aaatatttgc aagaggaaga aaacagtcca ccaagtgaca 241 gcactattcc aggcatacag aaaatttgga tacgaacatg gggttgttct cataataatt 301 cagatggaga atatatggct ggacagctag ctgcttatgg ctataaaatt acagaaaatg 361 catccgatgc agatttatgg ctcctgaaca gttgcactgt aaaaaaccca gctgaagacc 421 actttagaaa ctcaattaaa aaagctcaag aggagaacaa gaaaatcgta ctggctggat 481 gcgttcctca agcccagcct cgccaggact accttaaggg actgagtatc attggggttc 541 agcagataga tcgtgtggta gaagttgtgg aggagacaat taaaggtcac tctgtgagac 601 tgctgggtca gaaaaaggat aatggaaggc ggcttggggg agcacgattg gatttgccga 661 agattaggaa gaatccactg atagaaatca tttccatcaa taccgggtgt ctcaatgctt 721 gtacctactg caaaactaaa cacgccagag gaaatttggc cagttatcca attgatgaac 781 tagtagatag agccaaacaa tcttttcaag agggtgtttg tgagatatgg ttgaccagtg 841 aagacacggg ggcttatggc agagatattg gcaccaatct ccccacactc ctgtggaaac 901 tggttgaagt gattcctgag ggagcaatgc tgaggcttgg catgacaaat ccgccctata 961 ttttagagca tctggaggaa atggcaaaaa tccttaatca ccccagagtc tacgcttttc 1021 tgcacatacc agtccagtct gcctccgaca gcgtactcat ggaaatgaaa agagaatact 1081 gtgtggctga cttcaaaaga gtagtggatt ttctgaaaga gaaagttcct ggaataacta 1141 ttgctacaga tattatctgt ggttttcctg gagaaacaga tcaggatttt caagaaacag 1201 tgaaacttgt tgaagagtac aaattcccaa gcctgtttat taaccaattt tacccaagac 1261 caggaactcc tGCTGCAAAA ATGGAACAAG TTCCAGCACA AGTGAAAAAG CAAAGGACAA 1321 AAGATCTTTC TCGGGTGTTT CATTCTTACA GTCCATATGA TCACAAGATT GGTGAAAGAC 1381 AACAAGTGTT AGTAACAGAA GAATCTTTTG ATTCCAAGTT TTATGTTGCA CACAATCAAT 1441 TCTATGAGCA GGTTTTAGTG CCAAAGAACC CTGCGTTCAT GGGGAAGATG GTTGAAGTGG 1501 ACATCTATGA ATCAGGCAAA CATTTTATGA AAGGGCAGCC AGTATCTGAT GCCAAAGTGT 1561 ACACGCCCTC CATCAGCAAA CCGCTAGCAA AGGGAGAAGT CTCGGGTTTG ACAAAGGACT 1621 TCAGAAATGG GCTTGGGAAC CAGCTGAGTT CAGGATCCCA CACCTCTGCT GCATCTCAGT 1681 GTGACTCAGC GAGTTCCAGA ATGGTGCTGC CCATGCCAAG GCTACATCAA GACTGTGCGC 1741 TGAGGATGTC CGTGGGCTTG GCTCTGCTGG GTCTTCTTTT TGCTTTTTTT GTCAAGGTCT 1801 ATAATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1861 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1921 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACTTGGTCC AGACCCATGA TCACAACGCG 1981 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt