Construct: ORF TRCN0000475489

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF002082.1_s317c1
• Derived from: ccsbBroadEn_13930
• DNA Barcode: TTTAGGTATAAGCGGCATATCGAG
• Epitope Tag: V5 (not translated due to frame shift)
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: PSG2 (5670)

Vector Information:

• Vector Backbone: pLX_317
• Pol II Cassette 1: SV40-PuroR
• Pol II Cassette 2: EF1a-TRCN0000475489
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	5670	PSG2	pregnancy specific beta-1-g...	NM_031246.4	99.6%	98.8%	(many diffs)
2	human	5680	PSG11	pregnancy specific beta-1-g...	NM_002785.3	94%	88.7%	(many diffs)
3	human	440533	PSG8	pregnancy specific beta-1-g...	XM_011526986.1	91.3%	85.6%	(many diffs)
4	human	5672	PSG4	pregnancy specific beta-1-g...	NM_213633.3	90.4%	84.1%	(many diffs)
5	human	5669	PSG1	pregnancy specific beta-1-g...	XM_011527125.2	89.9%	84.1%	(many diffs)
6	human	5678	PSG9	pregnancy specific beta-1-g...	NM_001301708.2	89.7%	77.5%	(many diffs)
7	human	5672	PSG4	pregnancy specific beta-1-g...	XM_017027002.2	88.8%	83.5%	(many diffs)
8	human	5671	PSG3	pregnancy specific beta-1-g...	XM_011527126.2	87.3%	80.1%	(many diffs)
9	human	5673	PSG5	pregnancy specific beta-1-g...	NM_001130014.2	87.3%	74.7%	(many diffs)
10	human	5673	PSG5	pregnancy specific beta-1-g...	NM_002781.4	87.3%	74.7%	(many diffs)
11	human	5673	PSG5	pregnancy specific beta-1-g...	XM_017027003.1	87.3%	74.7%	(many diffs)
12	human	5669	PSG1	pregnancy specific beta-1-g...	NM_001330524.2	85%	73.4%	(many diffs)
13	human	5669	PSG1	pregnancy specific beta-1-g...	XM_005259065.4	83.7%	72.9%	(many diffs)
14	human	5672	PSG4	pregnancy specific beta-1-g...	NM_001276495.1	83.3%	72.2%	(many diffs)
15	human	5678	PSG9	pregnancy specific beta-1-g...	NM_001301707.2	82.7%	66.1%	(many diffs)
16	human	5672	PSG4	pregnancy specific beta-1-g...	XM_017027001.2	81.9%	71.7%	(many diffs)
17	human	5678	PSG9	pregnancy specific beta-1-g...	XM_005259075.4	75.9%	66.1%	(many diffs)
18	human	5671	PSG3	pregnancy specific beta-1-g...	NM_021016.4	72.8%	66.5%	(many diffs)
19	human	5669	PSG1	pregnancy specific beta-1-g...	NM_001297773.2	72%	65.2%	(many diffs)
20	human	440533	PSG8	pregnancy specific beta-1-g...	NM_182707.3	71.6%	66%	(many diffs)
21	human	5669	PSG1	pregnancy specific beta-1-g...	NM_001184825.2	71.5%	66.1%	(many diffs)
22	human	440533	PSG8	pregnancy specific beta-1-g...	NM_001130167.2	71.5%	66.8%	(many diffs)
23	human	5669	PSG1	pregnancy specific beta-1-g...	NM_001184826.2	71.4%	65.6%	(many diffs)
24	human	5676	PSG7	pregnancy specific beta-1-g...	NM_002783.3	71.1%	67%	(many diffs)
25	human	5672	PSG4	pregnancy specific beta-1-g...	NM_002780.5	70.7%	65.6%	(many diffs)
26	human	5669	PSG1	pregnancy specific beta-1-g...	NM_006905.3	70.7%	65.8%	(many diffs)
27	human	5675	PSG6	pregnancy specific beta-1-g...	NM_001031850.4	70.4%	63.6%	(many diffs)
28	human	5678	PSG9	pregnancy specific beta-1-g...	NM_002784.5	70.3%	60.7%	(many diffs)
29	human	5678	PSG9	pregnancy specific beta-1-g...	XM_017027005.2	70.2%	56.5%	(many diffs)
30	human	5675	PSG6	pregnancy specific beta-1-g...	NM_002782.5	69.8%	63.1%	(many diffs)
31	human	5672	PSG4	pregnancy specific beta-1-g...	XM_017026997.2	69.8%	65.3%	(many diffs)
32	human	5678	PSG9	pregnancy specific beta-1-g...	XM_017027007.2	69.1%	60.6%	(many diffs)
33	human	5671	PSG3	pregnancy specific beta-1-g...	XM_011527127.2	65.9%	58.6%	(many diffs)
34	human	5678	PSG9	pregnancy specific beta-1-g...	NM_001301709.2	63.9%	54.2%	(many diffs)
35	human	5678	PSG9	pregnancy specific beta-1-g...	XM_017027004.2	61.6%	53.6%	(many diffs)
36	human	5680	PSG11	pregnancy specific beta-1-g...	NM_001113410.2	58.6%	53.3%	(many diffs)
37	human	5680	PSG11	pregnancy specific beta-1-g...	NM_203287.2	58.6%	53.3%	(many diffs)
38	human	5678	PSG9	pregnancy specific beta-1-g...	XM_017027006.2	54.3%	46.4%	(many diffs)
39	human	653492	PSG10P	pregnancy specific beta-1-g...	NR_026824.1	49.6%		(many diffs)
40	human	440533	PSG8	pregnancy specific beta-1-g...	XM_011526987.3	43.5%	37.3%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1071

ORF length:

1005

Sequence:

1	tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61	ttggcatggg gcccctctca gcccctccct gcacagagca catcaaatgg aaggggctcc
121	tgctcacagc atcactttta aacttctgga acctgcccac cactgcccaa gtcacgattg
181	aagcccagcc accaaaagtt tccgagggga aggatgttct tctacttgtc cacaatttgc
241	cccagaatct tactggctac atctggtaca aagggcaaat cagggacctc taccattaca
301	ttacatcata tgtagtagac ggtcaaataa ttatatatgg gcctgcatat agtggacgag
361	aaacagcata ttccaatgca tccctgctga tccagaatgt cacccgggag gacgcaggat
421	cctacacctt acacatcata aagcgaggtg atgggactag aggagtaact ggatatttca
481	ccttcacctt atacctggag actcccaagc cctccatctc cagcagcaac ttaaacccca
541	gggaggccat ggaaactgtg atcttaacct gtgatcctga gactccggac acaagctacc
601	agtggtggat gaatggtcag agcctcccta tgactcacag gtttcagctg tccgaaacca
661	acaggaccct ctttctattt ggtgtcacaa agtatactgc aggaccctat gaatgtgaaa
721	tacggaactc agggagtgcc agccgcagtg acccagtcac ccTGAATCTC CTCCATGGTC
781	CAGACCTCCC CAGAATTCAC CCTTCATACA CCAATTACCG TTCAGGAGAT AACCTCTACT
841	TGTCTTGCTT CGCGAACTCT AACCCACCGG CACAGTATTC TTGGACAATT AATGGGAAGT
901	TTCAGCAATC AGGACAAAAT CTGTTTATCC CCCAAATTAC TACAAAGCAT AGCGGGCTCT
961	ATGTTTGCTC TGTTCGTAAC TCAGCCACTG GCGAGGAAAG CTCCACGTCG TTGACAGTCA
1021	AAGTCTCTGC TTCTACAAGA ATAGGACTTC TTCCTCTCCT TATCCAACAT ACCCAACTTT
1081	CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
1141	TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC
1201	TTGTGGAAAG GACGATTTAG GTATAAGCGG CATATCGAGA CGCGTTAAGT Cgacaatcaa
1261	cctctggatt acaaaatttg tgaaagatt

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