Construct: ORF TRCN0000477990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004983.1_s317c1
- Derived from:
- ccsbBroadEn_00841
- DNA Barcode:
- GGTACCTCTGAAAACAACGAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IKBKB (3551)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477990
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273494.3 | 58.6% | 57% | (many diffs) |
| 2 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273492.4 | 56% | 54.4% | (many diffs) |
| 3 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001556.3 | 52.3% | 50.8% | (many diffs) |
| 4 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273490.3 | 49.2% | 47.7% | (many diffs) |
| 5 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544517.2 | 49.2% | 47.7% | (many diffs) |
| 6 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001190720.2 | 48.6% | 45.9% | (many diffs) |
| 7 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NM_001242778.2 | 44.6% | 42.1% | (many diffs) |
| 8 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544518.2 | 43.1% | 39.5% | (many diffs) |
| 9 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273491.5 | 42% | 39.6% | (many diffs) |
| 10 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544519.2 | 42% | 39.6% | (many diffs) |
| 11 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544520.2 | 37.2% | 31.5% | (many diffs) |
| 12 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273493.4 | 36.3% | 32% | (many diffs) |
| 13 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XR_001745530.2 | 31.6% | (many diffs) | |
| 14 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_040009.2 | 31.5% | (many diffs) | |
| 15 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_033819.2 | 30.8% | (many diffs) | |
| 16 | human | 3551 | IKBKB | inhibitor of nuclear factor... | NR_033818.2 | 29.9% | (many diffs) | |
| 17 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XR_949402.3 | 24.8% | (many diffs) | |
| 18 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273495.2 | 23.4% | 19.3% | (many diffs) |
| 19 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273496.4 | 23.4% | 19.3% | (many diffs) |
| 20 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_005273498.4 | 23.4% | 19.3% | (many diffs) |
| 21 | human | 3551 | IKBKB | inhibitor of nuclear factor... | XM_011544521.2 | 23.4% | 19.3% | (many diffs) |
| 22 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509023.3 | 81.6% | 84.4% | (many diffs) |
| 23 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509022.3 | 66.7% | 68.4% | (many diffs) |
| 24 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509021.2 | 64.8% | 66.5% | (many diffs) |
| 25 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509020.3 | 50.1% | 51.2% | (many diffs) |
| 26 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | NM_010546.2 | 47.9% | 48.9% | (many diffs) |
| 27 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | XM_006509019.3 | 47.6% | 48.6% | (many diffs) |
| 28 | mouse | 16150 | Ikbkb | inhibitor of kappaB kinase ... | NM_001159774.1 | 46.7% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1329
- ORF length:
- 1260
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctggtca ccttccctga caacgcagac atgtggggcc tgggaaatga 121 aagagcgcct tgggacaggg ggatttggaa atgtcatccg atggcacaat caggaaacag 181 gtgagcagat tgccatcaag cagtgccggc aggagctcag cccccggaac cgagagcggt 241 ggtgcctgga gatccagatc atgagaaggc tgacccaccc caatgtggtg gctgcccgag 301 atgtccctga ggggatgcag aacttggcgc ccaatgacct gcccctgctg gccatggagt 361 actgccaagg aggagatctc cggaagtacc tgaaccagtt tgagaactgc tgtggtctgc 421 gggaaggtgc catcctcacc ttgctgagtg acattgcctc tgcgcttaga taccttcatg 481 aaaacagaat catccatcgg gatctaaagc cagaaaacat cgtcctgcag caaggagaac 541 agaggttaat acacaaaatt attgacctag gatatgccaa ggagctggat cagggcagtc 601 tttgcacatc attcgtgggg accctgcagt acctggcccc agagctactg gagcagcaga 661 agtacacagt gaccgtcgac tactggagct tcggcaccct ggcctttgag tgcatcacgg 721 gcttccggcc cttcctcccc aactggcagc ccgtgcagtg gcattcaaaa gtgcggcaga 781 agagtgaggt ggacattgtt gttagcgaag acttgaatgg aacggtgaag ttttcaagct 841 ctttacccta ccccaataat cttaacagtg tcctggctga gcgactggag aagtggctgc 901 aactgatgct gatgtggCAC CCCCGACAGA GGGGCACGGA TCCCACGTAT GGGCCCAATG 961 GCTGCTTCAA GGCCCTGGAT GACATCTTAA ACTTAAAGCT GGTTCATATC TTGAACATGG 1021 TCACGGGCAC CATCCACACC TACCCTGTGA CAGAGGATGA GAGTCTGCAG AGCTTGAAGG 1081 CCAGAATCCA ACAGGACACG GGCATCCCAG AGGAGGACCA GGAGCTGCTG CAGGAAGCGG 1141 GCCTGGCGTT GATCCCCGAT AAGCCTGCCA CTCAGTGTAT TTCAGACGGC AAGGTGAGCC 1201 CTGGCTTCGT ACACACCATC CTGTTTACCT TGGCTGTGCC TCCTGGGAAA CTCAACACAC 1261 TTTCAGATTT CAATTCTGCT TTGTCATGTA GTCTGTTAAT CACACAGTGC GGATACCTGG 1321 CTGGTTTTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGTACC TCTGAAAACA ACGAACATAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt