Construct: ORF TRCN0000478211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013989.1_s317c1
- Derived from:
- ccsbBroadEn_12296
- DNA Barcode:
- CAAATCTCTGTTGACCAGACGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF253 (56242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_001331134.1 | 100% | 100% | |
| 2 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_001331133.1 | 97.2% | 97.2% | 1_36del |
| 3 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_021047.3 | 84.7% | 84.7% | 1_228del |
| 4 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_017026117.2 | 74.3% | 62.7% | (many diffs) |
| 5 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_017026116.1 | 71.7% | 60.5% | (many diffs) |
| 6 | human | 440515 | ZNF506 | zinc finger protein 506 | NM_001145404.2 | 69.2% | 59.1% | (many diffs) |
| 7 | human | 199777 | ZNF626 | zinc finger protein 626 | NM_001076675.3 | 68.9% | 58.1% | (many diffs) |
| 8 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256173.1 | 68.2% | 56.3% | (many diffs) |
| 9 | human | 7639 | ZNF85 | zinc finger protein 85 | XM_011528264.1 | 67.5% | 55.7% | (many diffs) |
| 10 | human | 100129842 | ZNF737 | zinc finger protein 737 | NM_001159293.2 | 67.4% | 56.9% | (many diffs) |
| 11 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_006722597.4 | 65.7% | 55.4% | (many diffs) |
| 12 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_011527599.3 | 65.7% | 55.4% | (many diffs) |
| 13 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_005259697.4 | 65.6% | 55.3% | (many diffs) |
| 14 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_011527598.3 | 65.6% | 55.3% | (many diffs) |
| 15 | human | 440515 | ZNF506 | zinc finger protein 506 | NM_001099269.3 | 64.8% | 55.4% | (many diffs) |
| 16 | human | 90649 | ZNF486 | zinc finger protein 486 | NM_052852.4 | 63.3% | 52.4% | (many diffs) |
| 17 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_003429.5 | 60.9% | 50.2% | (many diffs) |
| 18 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256171.1 | 57.9% | 47.8% | (many diffs) |
| 19 | human | 7639 | ZNF85 | zinc finger protein 85 | XM_011528263.2 | 56.6% | 46.7% | (many diffs) |
| 20 | human | 100129842 | ZNF737 | zinc finger protein 737 | XR_001753570.2 | 50.5% | (many diffs) | |
| 21 | human | 7608 | ZNF56 | zinc finger protein 56 | NM_001355194.2 | 49% | 38.6% | (many diffs) |
| 22 | human | 284443 | ZNF493 | zinc finger protein 493 | NM_175910.7 | 46.9% | 37.5% | (many diffs) |
| 23 | human | 105372319 | LOC105372319 | zinc finger protein 430-like | XM_017027571.1 | 38.1% | 25.5% | (many diffs) |
| 24 | human | 664701 | ZNF826P | zinc finger protein 826, ps... | NR_036455.1 | 21.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttctcatttt gcccaagacc tttggccaga gaacatacaa aattctttcc 121 aaatagggat gctgagaaga tatgaagaat gcagacatga caatttacag ttaaaaaaag 181 gctgtaaaag cgtgggtgag cataaggtgc acaaaggagg ttataatgga cttaaccaat 241 gtttgacaac tacccagaaa gaaatatttc aatgtgataa atatggaaaa gtctttcata 301 agttttcaaa ttcaaacaca tataagacaa gacatactgg aataaatctt ttcaaatgta 361 taatatgtgg caaagctttt aaacggtcct caacccttac tacacataag aaaattcata 421 ctggagagaa accttacaga tgtgaagaat gtggcaaagc ttttaaccaa tctgcaaacc 481 ttactacaca taagagaatt cataccggag agaaacccta cagatgtgaa gaatgtggca 541 aagcctttaa gcagtcctca aaccttacta cacataagaa aattcatact ggagagaaac 601 cctacaaatg tgaagaatgt ggcaaagcct tcaaccgatc cacagacctt actacacata 661 agatagttca tactggagag aaaccctaca aatgtgaaga atgtggcaaa gcctttaagc 721 acccctcaca cgttaccaca cataagaaaa ttcatactag agggaaaccc tacaactgtg 781 aagaatgtgg caaatccttt aagcactgct ctaaccttac tatacataag agaattcata 841 caggagagaa accctacaaa tgtgaagaat gtggcaaagc ctttcaccta tcctcacacc 901 ttactacaca taagatactt catactggag agaaacccta cagatgtaga gaatgtggca 961 aagcttttaa ccattccaca accctttttt cacatgagaa aattcatact ggagagaaac 1021 cctacaaatg tgatgaatgt ggcaaaacct ttacctggcc ctcaatcctc tccaaacata 1081 aaagaactca tactGGAGAG AAACCCTACA AATGTGAAGA ATGTGGCAAA TCCTTTACTG 1141 CATCCTCAAC TCTAACTACA CATAAGAGAA TTCATACTGG AGAGAAACCT TACAAATGTG 1201 AAGAATGTGG CAAAGCTTTT AACTGGTCCT CAGACCTTAA TAAACATAAG AAAATTCATA 1261 TTGAACGAAA ACCCTACATA GTGAAGAATG TGACAGATCT TTTAAATGTT CCTCCACTTT 1321 TAATTAGCAT AAGATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CAAATCTCTG TTGACCAGAC 1501 GGTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt