Transcript: Human XM_011528264.1

PREDICTED: Homo sapiens zinc finger protein 85 (ZNF85), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF85 (7639)
Length:
2652
CDS:
647..2257

Additional Resources:

NCBI RefSeq record:
XM_011528264.1
NBCI Gene record:
ZNF85 (7639)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011528264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434187 GTCACACTTTAGGTAAGATAA pLKO_005 2442 3UTR 100% 13.200 9.240 N ZNF85 n/a
2 TRCN0000015351 ACACTCTTCAACCCTTACTAA pLKO.1 1693 CDS 100% 5.625 3.938 N ZNF85 n/a
3 TRCN0000015349 GCCTAACTGAACATAGCAGAA pLKO.1 1032 CDS 100% 4.050 2.835 N ZNF85 n/a
4 TRCN0000015348 CCTGAAAGATGTGACAATAAT pLKO.1 2334 3UTR 100% 15.000 9.000 N ZNF85 n/a
5 TRCN0000431759 CATGAGATAAGACATACTAAA pLKO_005 959 CDS 100% 13.200 7.920 N ZNF85 n/a
6 TRCN0000015352 CGGGAGAGAAACCTTACAAAT pLKO.1 1143 CDS 100% 13.200 7.920 N ZNF85 n/a
7 TRCN0000430017 CCAGTCCTCAAACCTTATTAA pLKO_005 1189 CDS 100% 15.000 7.500 Y ZNF431 n/a
8 TRCN0000236731 ACCTTACTACACATAAGATAA pLKO_005 1452 CDS 100% 13.200 6.600 Y ZNF98 n/a
9 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1227 CDS 100% 13.200 6.600 Y Zfp934 n/a
10 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1227 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
11 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1227 CDS 100% 13.200 6.600 Y EG668616 n/a
12 TRCN0000146802 CCTCAAACCTTACTACACATA pLKO.1 1446 CDS 100% 4.950 2.475 Y ZNF714 n/a
13 TRCN0000243782 TGGAGAGAAACCCTATGAATA pLKO_005 1816 CDS 100% 13.200 6.600 Y Zfp977 n/a
14 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1148 CDS 100% 4.950 2.475 Y ZNF28 n/a
15 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 2100 CDS 100% 4.950 2.475 Y ZNF714 n/a
16 TRCN0000019028 GCCTTTAACCAGTCCTCAATT pLKO.1 1181 CDS 100% 13.200 6.600 Y ZNF92 n/a
17 TRCN0000240204 ATACGGGAGAGAAACCTTATG pLKO_005 1140 CDS 100% 10.800 5.400 Y EG665449 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011528264.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11232 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_11232 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000476048 TACTATCCCAATAGAAACACCTCC pLX_317 21.8% 100% 100% V5 n/a
4 ccsbBroadEn_12296 pDONR223 100% 67.5% 55.7% None (many diffs) n/a
5 TRCN0000478211 CAAATCTCTGTTGACCAGACGGTG pLX_317 19.4% 67.5% 55.7% V5 (many diffs) n/a
6 ccsbBroadEn_09784 pDONR223 100% 65.4% 56.4% None (many diffs) n/a
7 ccsbBroad304_09784 pLX_304 0% 65.4% 56.4% V5 (many diffs) n/a
8 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 65.4% 56.4% V5 (many diffs) n/a
9 TRCN0000477054 GAGCCAATTTATTAAACTTAACTA pLX_317 14.1% 57% 48.7% V5 (many diffs) n/a
10 ccsbBroadEn_15067 pDONR223 92.3% 56.9% 49% None (many diffs) n/a
11 ccsbBroad304_15067 pLX_304 0% 56.9% 49% V5 (not translated due to prior stop codon) (many diffs) n/a
12 ccsbBroadEn_07157 pDONR223 100% 55.4% 47.7% None (many diffs) n/a
13 ccsbBroad304_07157 pLX_304 0% 55.4% 47.7% V5 (many diffs) n/a
14 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 55.4% 47.7% V5 (many diffs) n/a
15 ccsbBroadEn_11235 pDONR223 100% 27.7% 22.5% None (many diffs) n/a
16 ccsbBroad304_11235 pLX_304 0% 27.7% 22.5% V5 (many diffs) n/a
17 TRCN0000481650 TTTATCAAAGGAATCCCCAATTTT pLX_317 100% 27.7% 22.5% V5 (many diffs) n/a
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