Construct: ORF TRCN0000487983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020464.1_s317c1
- DNA Barcode:
- GCAAGTCAACACTGGCGGTTTTAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAK5 (57144)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | NM_020341.4 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 2 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | NM_177990.3 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 3 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027960.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 4 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027961.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 5 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027962.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 6 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027963.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 7 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027964.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 8 | human | 57144 | PAK5 | p21 (RAC1) activated kinase 5 | XM_017027965.1 | 98.5% | 98.4% | 1532G>A;1837_1866del |
| 9 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | NM_172858.2 | 88.3% | 92.6% | (many diffs) |
| 10 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499509.1 | 88.3% | 92.6% | (many diffs) |
| 11 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_011239531.1 | 88.3% | 92.6% | (many diffs) |
| 12 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318217.1 | 88.3% | 92.6% | (many diffs) |
| 13 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318218.1 | 88.3% | 92.6% | (many diffs) |
| 14 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499508.3 | 86.3% | 90.4% | (many diffs) |
| 15 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_017318219.1 | 56.4% | 60.7% | (many diffs) |
| 16 | mouse | 241656 | Pak7 | p21 protein (Cdc42/Rac)-act... | XM_006499510.3 | 55.1% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2199
- ORF length:
- 2127
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtttggg aagaaaaaga aaaagattga aatatctggc ccgtccaact 121 ttgaacacag ggttcatact gggtttgatc cacaagagca gaagtttacc ggccttcccc 181 agcagtggca cagcctgtta gcagatacgg ccaacaggcc aaagcctatg gtggaccctt 241 catgcatcac acccatccag ctggctccta tgaagacaat cgttagagga aacaaaccct 301 gcaaggaaac ctccatcaac ggcctgctag aggattttga caacatctcg gtgactcgct 361 ccaactccct aaggaaagaa agcccaccca ccccagatca gggagcctcc agccacggtc 421 caggccacgc ggaagaaaat ggcttcatca ccttctccca gtattccagc gaatccgata 481 ctactgctga ctacacgacc gaaaagtaca gggagaagag tctctatgga gatgatctgg 541 atccgtatta tagaggcagc cacgcagcca agcaaaatgg gcacgtaatg aaaatgaagc 601 acggggaggc ctactattct gaggtgaagc ctttgaaatc cgattttgcc agattttctg 661 ccgattatca ctcacatttg gactcactga gcaaaccaag tgaatacagt gacctcaagt 721 gggagtatca gagagcctcg agtagctccc ctctggatta ttcattccaa ttcacacctt 781 ctagaactgc agggaccagc gggtgctcca aggagagcct ggcgtacagt gaaagtgaat 841 ggggacccag cctggatgac tatgacagga ggccaaagtc ttcgtacctg aatcagacaa 901 gccctcagcc caccatgcgg cagaggtcca ggtcaggctc gggactccag gaaccgatga 961 tgccatttgg agcaagtgca tttaaaaccc atccccaagg acactcctac aactcctaca 1021 cctaccctcg cttgtccgag cccacaatgt gcattccaaa ggtggattac gatcgagcac 1081 agatggtcct cagccctcca ctgtcagggt ctgacaccta ccccaggggc cctgccaaac 1141 tacctcaaag tcaaagcaaa tcgggctatt cctcaagcag tcaccagtac ccgtctgggt 1201 accacaaagc caccttgtac catcacccct ccctgcagag cagttcgcag tacatctcca 1261 cggcttccta cctgagctcc ctcagcctct catccagcac ctacccgccg cccagctggg 1321 gctcctcctc cgaccagcag ccctccaggg tgtcccatga acagtttcgg gcggccctgc 1381 agctggtggt cagcccagga gaccccaggg aatacttggc caactttatc aaaatcgggg 1441 aaggctcaac cggcatcgta tgcatcgcca ccgagaaaca cacagggaaa caagttgcag 1501 tgaagaaaat ggacctccgg aagcaacaga gacgagaact gcttttcaat gaggtcgtga 1561 tcatgcggga ttaccaccat gacaatgtgg ttgacatgta caacagctac cttgtcggcg 1621 atgagctctg ggtggtcatg gagtttctag aaggtggtgc cttgacagac attgtgactc 1681 acaccagaat gaatgaagaa cagatagcta ctgtctgcct gtcagttctg agagctctct 1741 cctaccttca taaccaagga gtgattcaca gggacataaa aagtgactcc atcctcctga 1801 caagcgatgg ccggataaag ttgtctgatt ttggtttctg tgctcaagtt tccaaagagg 1861 tgccgaagag gaaatcattg gttggcactc cctactggat ggccccTGAG GTGGACATCT 1921 GGTCCCTCGG GATCATGGTG ATAGAAATGA TTGATGGCGA GCCCCCCTAC TTCAATGAGC 1981 CTCCCCTCCA GGCGATGCGG AGGATCCGGG ACAGTTTACC TCCAAGAGTG AAGGACCTAC 2041 ACAAGGTTTC TTCAGTGCTC CGGGGATTCC TAGACTTGAT GTTGGTGAGG GAGCCCTCTC 2101 AGAGAGCAAC AGCCCAGGAA CTCCTCGGAC ATCCATTCTT AAAACTAGCA GGTCCACCGT 2161 CTTGCATCGT CCCCCTCATG AGACAATACA GGCATCACTA GGACCCAGCT TTCTTGTACA 2221 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2281 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2341 AGGACGAGCA AGTCAACACT GGCGGTTTTA GACGCGTTAA GTCgacaatc aacctctgga 2401 ttacaaaatt tgtgaaagat t