Construct: ORF TRCN0000487983

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020464.1_s317c1
DNA Barcode:
GCAAGTCAACACTGGCGGTTTTAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PAK5 (57144)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000487983
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57144 PAK5 p21 (RAC1) activated kinase 5 NM_020341.4 98.5% 98.4% 1532G>A;1837_1866del
2 human 57144 PAK5 p21 (RAC1) activated kinase 5 NM_177990.3 98.5% 98.4% 1532G>A;1837_1866del
3 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027960.1 98.5% 98.4% 1532G>A;1837_1866del
4 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027961.1 98.5% 98.4% 1532G>A;1837_1866del
5 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027962.1 98.5% 98.4% 1532G>A;1837_1866del
6 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027963.1 98.5% 98.4% 1532G>A;1837_1866del
7 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027964.1 98.5% 98.4% 1532G>A;1837_1866del
8 human 57144 PAK5 p21 (RAC1) activated kinase 5 XM_017027965.1 98.5% 98.4% 1532G>A;1837_1866del
9 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... NM_172858.2 88.3% 92.6% (many diffs)
10 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_006499509.1 88.3% 92.6% (many diffs)
11 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_011239531.1 88.3% 92.6% (many diffs)
12 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_017318217.1 88.3% 92.6% (many diffs)
13 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_017318218.1 88.3% 92.6% (many diffs)
14 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_006499508.3 86.3% 90.4% (many diffs)
15 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_017318219.1 56.4% 60.7% (many diffs)
16 mouse 241656 Pak7 p21 protein (Cdc42/Rac)-act... XM_006499510.3 55.1% 59.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2199
ORF length:
2127
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtttggg aagaaaaaga aaaagattga aatatctggc ccgtccaact
121ttgaacacag ggttcatact gggtttgatc cacaagagca gaagtttacc ggccttcccc
181agcagtggca cagcctgtta gcagatacgg ccaacaggcc aaagcctatg gtggaccctt
241catgcatcac acccatccag ctggctccta tgaagacaat cgttagagga aacaaaccct
301gcaaggaaac ctccatcaac ggcctgctag aggattttga caacatctcg gtgactcgct
361ccaactccct aaggaaagaa agcccaccca ccccagatca gggagcctcc agccacggtc
421caggccacgc ggaagaaaat ggcttcatca ccttctccca gtattccagc gaatccgata
481ctactgctga ctacacgacc gaaaagtaca gggagaagag tctctatgga gatgatctgg
541atccgtatta tagaggcagc cacgcagcca agcaaaatgg gcacgtaatg aaaatgaagc
601acggggaggc ctactattct gaggtgaagc ctttgaaatc cgattttgcc agattttctg
661ccgattatca ctcacatttg gactcactga gcaaaccaag tgaatacagt gacctcaagt
721gggagtatca gagagcctcg agtagctccc ctctggatta ttcattccaa ttcacacctt
781ctagaactgc agggaccagc gggtgctcca aggagagcct ggcgtacagt gaaagtgaat
841ggggacccag cctggatgac tatgacagga ggccaaagtc ttcgtacctg aatcagacaa
901gccctcagcc caccatgcgg cagaggtcca ggtcaggctc gggactccag gaaccgatga
961tgccatttgg agcaagtgca tttaaaaccc atccccaagg acactcctac aactcctaca
1021cctaccctcg cttgtccgag cccacaatgt gcattccaaa ggtggattac gatcgagcac
1081agatggtcct cagccctcca ctgtcagggt ctgacaccta ccccaggggc cctgccaaac
1141tacctcaaag tcaaagcaaa tcgggctatt cctcaagcag tcaccagtac ccgtctgggt
1201accacaaagc caccttgtac catcacccct ccctgcagag cagttcgcag tacatctcca
1261cggcttccta cctgagctcc ctcagcctct catccagcac ctacccgccg cccagctggg
1321gctcctcctc cgaccagcag ccctccaggg tgtcccatga acagtttcgg gcggccctgc
1381agctggtggt cagcccagga gaccccaggg aatacttggc caactttatc aaaatcgggg
1441aaggctcaac cggcatcgta tgcatcgcca ccgagaaaca cacagggaaa caagttgcag
1501tgaagaaaat ggacctccgg aagcaacaga gacgagaact gcttttcaat gaggtcgtga
1561tcatgcggga ttaccaccat gacaatgtgg ttgacatgta caacagctac cttgtcggcg
1621atgagctctg ggtggtcatg gagtttctag aaggtggtgc cttgacagac attgtgactc
1681acaccagaat gaatgaagaa cagatagcta ctgtctgcct gtcagttctg agagctctct
1741cctaccttca taaccaagga gtgattcaca gggacataaa aagtgactcc atcctcctga
1801caagcgatgg ccggataaag ttgtctgatt ttggtttctg tgctcaagtt tccaaagagg
1861tgccgaagag gaaatcattg gttggcactc cctactggat ggccccTGAG GTGGACATCT
1921GGTCCCTCGG GATCATGGTG ATAGAAATGA TTGATGGCGA GCCCCCCTAC TTCAATGAGC
1981CTCCCCTCCA GGCGATGCGG AGGATCCGGG ACAGTTTACC TCCAAGAGTG AAGGACCTAC
2041ACAAGGTTTC TTCAGTGCTC CGGGGATTCC TAGACTTGAT GTTGGTGAGG GAGCCCTCTC
2101AGAGAGCAAC AGCCCAGGAA CTCCTCGGAC ATCCATTCTT AAAACTAGCA GGTCCACCGT
2161CTTGCATCGT CCCCCTCATG AGACAATACA GGCATCACTA GGACCCAGCT TTCTTGTACA
2221AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
2281AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
2341AGGACGAGCA AGTCAACACT GGCGGTTTTA GACGCGTTAA GTCgacaatc aacctctgga
2401ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)