Construct: ORF ccsbBroad304_03879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017572.1_s304c1, BRDN0000408719
- Derived from:
- ccsbBroadEn_03879
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALOXE3 (59344)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03879
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | NM_021628.2 | 100% | 100% | |
| 2 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | NM_001369446.1 | 99.8% | 99.8% | 551_552insCAG |
| 3 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XM_017024921.2 | 85.6% | 82.6% | (many diffs) |
| 4 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | NM_001165960.1 | 84.3% | 84.3% | 1_396del |
| 5 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XM_017024922.2 | 78.6% | 74.3% | (many diffs) |
| 6 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XR_001752580.2 | 77.8% | (many diffs) | |
| 7 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XM_017024923.2 | 74.9% | 72.2% | (many diffs) |
| 8 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XM_017024924.2 | 68.7% | 65.7% | 1389_1390ins89;1467_1468ins577 |
| 9 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XM_017024925.2 | 68.7% | 65.7% | 1389_1390ins89;1467_1468ins577 |
| 10 | human | 59344 | ALOXE3 | arachidonate lipoxygenase 3 | XR_001752579.2 | 64.1% | 1_273del;1833_1834ins122;2285_3014del | |
| 11 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | NM_011786.2 | 86.4% | 88.4% | (many diffs) |
| 12 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | XM_006533257.3 | 57.2% | 56.1% | (many diffs) |
| 13 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | XR_388449.2 | 55.7% | (many diffs) | |
| 14 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | XR_388452.3 | 54.3% | (many diffs) | |
| 15 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | XR_388450.2 | 51.1% | (many diffs) | |
| 16 | mouse | 23801 | Aloxe3 | arachidonate lipoxygenase 3 | XR_388451.2 | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2202
- ORF length:
- 2133
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtgtac cgcctgtgtg tgaccactgg tccctacctg agggccggca 121 cactggacaa catctctgtc acactggtgg gcacgtgtgg tgaaagcccc aagcagcggc 181 tagatcgaat gggcagggac ttcgcccctg gatcggtaca gaagtacaag gtgcgttgca 241 cagcggagct gggtgagctc ttgctgctgc gtgtacacaa ggagcgctac gctttcttcc 301 gcaaggactc ttggtactgt agccgcatct gtgtcaccga accggatggt agtgtatccc 361 acttcccctg ctatcagtgg attgaaggct actgcaccgt ggagctgagg ccaggaacag 421 caagaactat ttgtcaggac tctcttcccc tcctcctgga tcacaggaca cgggagctcc 481 gggcccgaca agaatgctac cgctggaaga tctatgcccc tggcttcccc tgcatggtag 541 acgtcaacag ctttcaggag atggagtcag acaagaaatt tgccttgaca aagacgacaa 601 cttgtgtaga ccagggtgac agcagtggga atcggtacct gcccggcttc cccatgaaaa 661 ttgacatccc atccctgatg tacatggagc ccaatgttcg atactcagcc accaagacga 721 tctcgctgct cttcaatgcc atccctgcgt ccttgggaat gaagcttcga gggctgttgg 781 atcgcaaggg ctcctggaag aagctggatg acatgcagaa catcttctgg tgccataaga 841 ccttcacgac aaagtatgtc acagagcact ggtgtgaaga tcacttcttt gggtaccagt 901 acctgaatgg tgtcaatccc gtcatgctcc actgcatctc tagcttgccc agcaagctgc 961 ctgtcaccaa tgacatggtg gcccccttgc tgggacagga cacatgcctg cagacagagc 1021 tagagagggg gaacatcttc ctagcggact actggatcct ggcggaggcc cccacccact 1081 gcctaaacgg ccgccagcag tacgtggccg ccccactgtg cctgctgtgg ctcagccccc 1141 agggggcgct ggtgcccttg gccatccagc tcagccagac ccccgggcct gacagcccca 1201 tcttcctgcc cactgactcc gaatgggact ggctgctggc caagacgtgg gtgcgcaact 1261 ctgagttcct ggtgcacgaa aacaacacgc actttctgtg cacgcatttg ctgtgcgagg 1321 ccttcgccat ggccacgctg cgccagctgc cgctctgcca ccccatctac aagctcctac 1381 tcccccacac tcgatacacg ctgcaggtga acaccatcgc gagggccacg ctgctcaacc 1441 ccgagggcct cgtggaccag gtcacgtcca tcgggaggca aggcctcatc tacctcatga 1501 gcacgggcct ggcccacttc acctacacca atttctgcct tccggacagc ctgcgggccc 1561 gcggcgtcct ggctatcccc aactaccact accgagacga cggcctgaag atctgggcgg 1621 ccattgagag ctttgtctca gaaatcgtgg gctactatta tcccagtgac gcatctgtgc 1681 agcaggattc ggagctgcag gcctggactg gcgagatttt tgctcaggcg ttcctgggcc 1741 gggaaagctc aggtttccca agccggctgt gcaccccagg agagatggtg aagttcctca 1801 ctgcaatcat cttcaattgc tctgcccagc acgctgctgt caacagtggg cagcatgact 1861 ttggggcctg gatgcccaat gctccatcat ccatgaggca gcccccaccc cagaccaagg 1921 ggaccaccac cctgaagact tacctagaca ccctccctga agtgaacatc agctgtaaca 1981 acctcctcct cttctggttg gttagccaag aacccaagga ccagaggccc ctgggcacct 2041 acccagatga gcacttcaca gaggaggccc cgaggcggag catcgccgcc ttccagagcc 2101 gcctggccca gatctcaagg gacatccagg agcggaacca gggtctggca ctgccctaca 2161 cctacctgga ccctcccctc attgagaaca gcgtctccat cttgccaact ttcttgtaca 2221 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2281 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta