Construct: ORF ccsbBroad304_05019

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008873.1_s304c1, BRDN0000410194
Derived from:
ccsbBroadEn_05019
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
B3GALNT2 (148789)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05019
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... NM_152490.5 99.9% 100% 321G>A
2 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XM_017000394.1 92.3% 92.4% 110_232del;444G>A
3 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XM_006711749.3 84% 84% (many diffs)
4 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XM_017000395.1 80.7% 80.9% (many diffs)
5 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XR_001736988.1 60.7% (many diffs)
6 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... NM_001277155.3 52.2% 51.9% (many diffs)
7 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XR_001736990.1 28.1% (many diffs)
8 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XR_001736987.1 27.4% (many diffs)
9 human 148789 B3GALNT2 beta-1,3-N-acetylgalactosam... XR_001736989.1 25.8% (many diffs)
10 mouse 97884 B3galnt2 UDP-GalNAc:betaGlcNAc beta ... NM_178640.2 86.1% 87.6% (many diffs)
11 mouse 97884 B3galnt2 UDP-GalNAc:betaGlcNAc beta ... XM_006516818.3 80.6% 81.3% (many diffs)
12 mouse 97884 B3galnt2 UDP-GalNAc:betaGlcNAc beta ... XR_873137.1 49.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1566
ORF length:
1500
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg aaactggctg gtgctgctgt gcccgtgtgt gctcggggcc gcgctgcacc
121tctggctgcg gctgcgctcc ccgccgcccg cctgcgcctc cggggccggc cctgcagatc
181agttggcctt atttcctcag tggaaatcta ctcactatga tgtggtagtt ggcgtgttgt
241cagctcgcaa taaccatgaa cttcgaaacg tgataagaag cacctggatg agacatttgc
301tacagcatcc cacattaagt caacgtgtgc ttgtgaagtt cataataggt gctcatggct
361gtgaagtgcc tgtggaagac agggaagatc cttattcctg taaactactc aacatcacaa
421atccagtttt gaatcaggaa attgaagcgt tcagtctgtc cgaagacact tcatcggggc
481tgcctgagga tcgagttgtc agcgtgagtt tccgagttct ctaccccatc gttattacca
541gtcttggagt gttctacgat gccaatgatg tgggtttcca gaggaacatc actgtcaaac
601tttatcaggc agaacaagag gaggccctct tcattgctcg cttcagtcct ccaagctgtg
661gtgtgcaggt gaacaagctg tggtacaagc ccgtggaaca attcatctta ccagagagct
721ttgaaggtac aatcgtgtgg gagagccaag acctccacgg ccttgtgtca agaaatctcc
781acaaagtgac agtgaatgat ggagggggag ttctcagagt cattacagct ggggagggtg
841cattgcctca tgaattcttg gaaggtgtgg agggagttgc aggtggtttt atatatacta
901ttcaggaagg tgatgctctc ttacacaacc ttcattctcg ccctcaaaga cttattgatc
961atataaggaa tctccatgag gaagatgcct tactgaagga ggaaagcagc atctatgatg
1021atattgtttt tgtggatgtt gtcgacactt atcgtaatgt tcctgcaaaa ttattgaact
1081tctatagatg gactgtggaa acaacgagct tcaatttgtt gctgaagaca gatgatgact
1141gttacataga cctcgaagct gtatttaata ggattgtcca aaagaatctg gatgggccta
1201atttttggtg gggaaatttc agactgaatt gggcagttga ccgaaccgga aagtggcagg
1261agttggagta cccgagcccc gcttaccctg cctttgcatg tgggtcagga tatgtgatct
1321ccaaggacat cgtcaagtgg ctggcaagca actcggggag gttaaagacc tatcagggtg
1381aagatgtaag catgggcatc tggatggctg ccataggacc taaaagatac caggacagtc
1441tgtggctgtg tgagaagacc tgtgagacag gaatgctgtc ttctcctcag tattctccgt
1501gggaactgac ggaactgtgg aaactgaagg aacggtgcgg tgatccttgt cgatgtcaag
1561caagataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1621gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1681acctctggat ta

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