Construct: ORF ccsbBroad304_05019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008873.1_s304c1, BRDN0000410194
- Derived from:
- ccsbBroadEn_05019
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B3GALNT2 (148789)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05019
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | NM_152490.5 | 99.9% | 100% | 321G>A |
| 2 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XM_017000394.1 | 92.3% | 92.4% | 110_232del;444G>A |
| 3 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XM_006711749.3 | 84% | 84% | (many diffs) |
| 4 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XM_017000395.1 | 80.7% | 80.9% | (many diffs) |
| 5 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XR_001736988.1 | 60.7% | (many diffs) | |
| 6 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | NM_001277155.3 | 52.2% | 51.9% | (many diffs) |
| 7 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XR_001736990.1 | 28.1% | (many diffs) | |
| 8 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XR_001736987.1 | 27.4% | (many diffs) | |
| 9 | human | 148789 | B3GALNT2 | beta-1,3-N-acetylgalactosam... | XR_001736989.1 | 25.8% | (many diffs) | |
| 10 | mouse | 97884 | B3galnt2 | UDP-GalNAc:betaGlcNAc beta ... | NM_178640.2 | 86.1% | 87.6% | (many diffs) |
| 11 | mouse | 97884 | B3galnt2 | UDP-GalNAc:betaGlcNAc beta ... | XM_006516818.3 | 80.6% | 81.3% | (many diffs) |
| 12 | mouse | 97884 | B3galnt2 | UDP-GalNAc:betaGlcNAc beta ... | XR_873137.1 | 49.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg aaactggctg gtgctgctgt gcccgtgtgt gctcggggcc gcgctgcacc 121 tctggctgcg gctgcgctcc ccgccgcccg cctgcgcctc cggggccggc cctgcagatc 181 agttggcctt atttcctcag tggaaatcta ctcactatga tgtggtagtt ggcgtgttgt 241 cagctcgcaa taaccatgaa cttcgaaacg tgataagaag cacctggatg agacatttgc 301 tacagcatcc cacattaagt caacgtgtgc ttgtgaagtt cataataggt gctcatggct 361 gtgaagtgcc tgtggaagac agggaagatc cttattcctg taaactactc aacatcacaa 421 atccagtttt gaatcaggaa attgaagcgt tcagtctgtc cgaagacact tcatcggggc 481 tgcctgagga tcgagttgtc agcgtgagtt tccgagttct ctaccccatc gttattacca 541 gtcttggagt gttctacgat gccaatgatg tgggtttcca gaggaacatc actgtcaaac 601 tttatcaggc agaacaagag gaggccctct tcattgctcg cttcagtcct ccaagctgtg 661 gtgtgcaggt gaacaagctg tggtacaagc ccgtggaaca attcatctta ccagagagct 721 ttgaaggtac aatcgtgtgg gagagccaag acctccacgg ccttgtgtca agaaatctcc 781 acaaagtgac agtgaatgat ggagggggag ttctcagagt cattacagct ggggagggtg 841 cattgcctca tgaattcttg gaaggtgtgg agggagttgc aggtggtttt atatatacta 901 ttcaggaagg tgatgctctc ttacacaacc ttcattctcg ccctcaaaga cttattgatc 961 atataaggaa tctccatgag gaagatgcct tactgaagga ggaaagcagc atctatgatg 1021 atattgtttt tgtggatgtt gtcgacactt atcgtaatgt tcctgcaaaa ttattgaact 1081 tctatagatg gactgtggaa acaacgagct tcaatttgtt gctgaagaca gatgatgact 1141 gttacataga cctcgaagct gtatttaata ggattgtcca aaagaatctg gatgggccta 1201 atttttggtg gggaaatttc agactgaatt gggcagttga ccgaaccgga aagtggcagg 1261 agttggagta cccgagcccc gcttaccctg cctttgcatg tgggtcagga tatgtgatct 1321 ccaaggacat cgtcaagtgg ctggcaagca actcggggag gttaaagacc tatcagggtg 1381 aagatgtaag catgggcatc tggatggctg ccataggacc taaaagatac caggacagtc 1441 tgtggctgtg tgagaagacc tgtgagacag gaatgctgtc ttctcctcag tattctccgt 1501 gggaactgac ggaactgtgg aaactgaagg aacggtgcgg tgatccttgt cgatgtcaag 1561 caagataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1621 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1681 acctctggat ta