Construct: ORF ccsbBroad304_09120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005923.1_s304c1, BRDN0000410797
- Derived from:
- ccsbBroadEn_09120
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMTC1 (83857)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09120
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_175861.3 | 99.9% | 99.8% | 954T>C;2116G>T |
| 2 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_024449218.1 | 90.9% | 90.9% | 407_408ins207;747T>C;1909G>T |
| 3 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_001193451.2 | 87.6% | 87.6% | 1_324del;1278T>C;2440G>T |
| 4 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | NM_001367875.2 | 81.9% | 81.8% | (many diffs) |
| 5 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020004.1 | 81.1% | 79% | (many diffs) |
| 6 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020005.1 | 79.8% | 79.8% | (many diffs) |
| 7 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020007.2 | 76.5% | 72.9% | (many diffs) |
| 8 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020003.1 | 76% | 75.9% | (many diffs) |
| 9 | human | 83857 | TMTC1 | transmembrane O-mannosyltra... | XM_017020006.1 | 52.2% | 51.9% | (many diffs) |
| 10 | mouse | 387314 | Tmtc1 | transmembrane and tetratric... | NM_198967.5 | 69.1% | 73% | (many diffs) |
| 11 | mouse | 387314 | Tmtc1 | transmembrane and tetratric... | NM_001347518.1 | 65.3% | 69% | (many diffs) |
| 12 | mouse | 387314 | Tmtc1 | transmembrane and tetratric... | XM_006507072.3 | 64.4% | 66.9% | (many diffs) |
| 13 | mouse | 387314 | Tmtc1 | transmembrane and tetratric... | XM_011241620.2 | 46.4% | 47.7% | (many diffs) |
| 14 | mouse | 387314 | Tmtc1 | transmembrane and tetratric... | XM_017321670.1 | 45.4% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2388
- ORF length:
- 2322
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa cccattctac tttcatgcag taaatataat tttacactgc ttagtgactc 121 ttgtgctgat gtacacctgt gataaaactg tcttcaagaa tcgtggactt gcttttgtaa 181 cggcattgct ttttgctgta catcctattc atactgaggc ggtggctggg atcgttggca 241 gagcggacgt gttagcgtgt ctgctgtttc tattggcctt tctctcgtac aacaggagtc 301 tggatcaggg ctgtgttggg ggaagtttcc cttccacggt gtctcccttc ttcttgctgc 361 tcagtttgtt tctggggacc tgtgcgatgc tggtgaaaga gacaggcatc acggtgtttg 421 gagtgtgctt ggtttatgac ctcttttccc tttccaacaa gcaagacaag tcgagcaatg 481 gggccctctg tccacgcagc ccacagcagc ccgggagccc ccagccctcc tcactgccag 541 gccatcctca ccgggagaat gggaagcagc agcggttccc tcacaaagga gcttggggtg 601 gctgccactc tccactgcca ccagaaccca agagcagtgg attcccagtg tccccacgag 661 ctgtgtggtc catgatgaga ttcctcacct attcctacct cttggccttc aatgtgtggc 721 ttctgcttgc acccgtgacc ctgtgctatg actggcaggt cggcagtatt cctctggtag 781 agaccatatg ggacatgcgg aacttagcca ccatctttct ggcggttgtg atggccttat 841 tgagcctgca ctgcttagca gcctttaaga gactggagca caaggaggtt ttagtcggct 901 tgttgttcct ggtgttcccg ttcattccag ccagcaacct cttcttcagg gtgggttttg 961 tggtggcgga gagagtcctt tacatgccta gcatgggcta ctgcatcctt tttgtgcacg 1021 gactgagcaa gctctgcact tggctgaatc gatgtggggc caccaccctg attgtgtcca 1081 ctgtgttgct gctgttgctt ttctcttgga aaactgtgaa acagaatgaa atttggctgt 1141 caagagagtc cctattcagg tctggagttc aaactctgcc ccacaatgcc aaggttcact 1201 acaactatgc caatttcctg aaggaccaag gtcggaacaa ggaagcgatc taccactaca 1261 gaacagctct caagttgtat ccacgccatg caagtgcgct caacaacctt ggaacactga 1321 cgagagacac agcagaggca aagatgtact atcagagggc tctccagctc catccacagc 1381 ataaccgggc tcttttcaat ctggggaatc tcctcaagtc ccaggagaaa aaggaagaag 1441 ctatcacctt actgaaggat tccatcaaat atggtccaga gtttgcagat gcatattcaa 1501 gcttagcttc gttattggct gagcaggagc ggtttaaaga agctgaagaa atataccaaa 1561 ctggaataaa gaactgtcca gacagctcag atttacacaa caactatggg gttttcttag 1621 ttgatactgg cttaccagaa aaggcagtgg cccattacca gcaggccatc aaacttagcc 1681 ccagtcatca cgtggccatg gtgaacttgg gaagactcta caggtcactg ggagagaaca 1741 gcatggctga agaatggtac aagcgcgccc tgcaggtggc acacaaagct gagatattgt 1801 cacctttggg agcactgtat tacaacactg gccgatacga agaggctttg cagatttacc 1861 aggaagctgc agcacttcag ccttctcaga gggagctccg cttggcactg gctcaggttt 1921 tggccgtgat gggtcagaca aaagaagctg aaaagatgac caatcacatt gtgtcagagg 1981 agaccggatg ccttgaatgc tatcgcctct tgtcagccat ctatagcaag caggagaacc 2041 acgacaaggc acttgatgct atagacaagg ctctccagct gaaaccaaag gacccaaaag 2101 tcatttctga actttttttc acaaaaggaa accaattaag agagcagaac cttctcgaca 2161 aagcttttga gagctataga ttggctgtgc aactaaaccc agaccaagca caggcctgga 2221 tgaacatggg tggcatccaa cacatcaagg gaaaatatgt gtctgcaaga gcttattatg 2281 agagagcctt acagctggtt ccagacagca aactgctgaa ggaaaatctt gccaaattgg 2341 atcgcctaga aaaacgatta caagaagttc gagaaaagga tcaaacatac ccaactttct 2401 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2461 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta