Construct: ORF ccsbBroad304_15300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019034.1_s304c1, BRDN0000459833
- Derived from:
- ccsbBroadEn_15300
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA6 (285220)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15300
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285220 | EPHA6 | EPH receptor A6 | NM_001080448.3 | 100% | 100% | |
| 2 | human | 285220 | EPHA6 | EPH receptor A6 | XM_006713592.3 | 98.6% | 98.6% | 1730_1777del |
| 3 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006210.1 | 94.9% | 94.8% | 1730_1777del;2434_2435ins126 |
| 4 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006212.1 | 86.7% | 85.7% | (many diffs) |
| 5 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006211.1 | 85.2% | 85.1% | 0_1ins425;26_66del;1346_1393del |
| 6 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006213.1 | 84.9% | 84.9% | 0_1ins468;1262_1309del |
| 7 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006214.1 | 66.7% | 66.4% | (many diffs) |
| 8 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006215.1 | 51.1% | 49% | (many diffs) |
| 9 | human | 285220 | EPHA6 | EPH receptor A6 | XR_924126.2 | 51% | (many diffs) | |
| 10 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006216.1 | 50.6% | 49.4% | (many diffs) |
| 11 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006217.1 | 49% | 48.9% | 0_1ins1723;3_4insAGAAA |
| 12 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006218.1 | 49% | 48.9% | 0_1ins1723;3_4insAGAAA |
| 13 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006219.1 | 47.3% | 47.3% | 1607_1609delGAA;1611_1612ins1782 |
| 14 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006220.1 | 45.7% | 44.5% | (many diffs) |
| 15 | human | 285220 | EPHA6 | EPH receptor A6 | XM_011512705.2 | 33.9% | 32.5% | (many diffs) |
| 16 | human | 285220 | EPHA6 | EPH receptor A6 | NM_001278301.2 | 33.3% | 33% | (many diffs) |
| 17 | human | 285220 | EPHA6 | EPH receptor A6 | XM_011512706.2 | 32.9% | 32.8% | 1113_1114insGCTTGCAGACCA;1117G>T;1119_1120ins2259 |
| 18 | human | 285220 | EPHA6 | EPH receptor A6 | XM_011512707.2 | 32.9% | 32.8% | 1114_1115insCTTGC;1116A>G;1119_1119delAins2267 |
| 19 | human | 285220 | EPHA6 | EPH receptor A6 | XM_017006223.1 | 32.4% | 27.2% | (many diffs) |
| 20 | human | 285220 | EPHA6 | EPH receptor A6 | NM_001278300.2 | 32.1% | 27.5% | (many diffs) |
| 21 | human | 285220 | EPHA6 | EPH receptor A6 | NM_173655.4 | 28.4% | 26.8% | (many diffs) |
| 22 | human | 285220 | EPHA6 | EPH receptor A6 | XR_001740110.1 | 25.9% | 1_43del;1773_1820del;3482_13069del | |
| 23 | mouse | 13840 | Epha6 | Eph receptor A6 | NM_007938.2 | 89.7% | 95% | (many diffs) |
| 24 | mouse | 13840 | Epha6 | Eph receptor A6 | XM_006521787.2 | 77.6% | 83.8% | (many diffs) |
| 25 | mouse | 13840 | Epha6 | Eph receptor A6 | XM_011245826.2 | 30.2% | 30.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3459
- ORF length:
- 3390
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaattcccc tcgcctccag ccgcgaggag ctccccggcg ccgcaggcag 121 cgtcctcctc cgaagcagct gcacctgcaa ctgggcagcc tggaccctcg tgccctgttc 181 ccgggacctc gcgcaggggg cgccccggga caccccctgc gggccgggtg gaggaggaag 241 aggaggagga ggaagaagac gtggacaagg acccccatcc tacccagaac acctgcctgc 301 gctgccgcca cttctcttta agggagagga aaagagagcc taggagaacc atggggggct 361 gcgaagtccg ggaatttctt ttgcaatttg gtttcttctt gcctctgctg acagcgtggc 421 caggcgactg cagtcacgtc tccaacaacc aagttgtgtt gcttgataca acaactgtac 481 tgggagagct aggatggaaa acatatccat taaatgggtg ggatgccatc actgaaatgg 541 atgaacataa taggcccatt cacacatacc aggtatgtaa tgtaatggaa ccaaaccaaa 601 acaactggct tcgtacaaac tggatctccc gtgatgcagc tcagaaaatt tatgtggaaa 661 tgaaattcac actaagggat tgtaacagca tcccatgggt cttggggact tgcaaagaaa 721 catttaatct gttttatatg gaatcagatg agtcccacgg aattaaattc aagccaaacc 781 agtatacaaa gatcgacaca attgctgctg atgagagttt tacccagatg gatttgggtg 841 atcgcatcct caaactcaac actgaaattc gtgaggtggg gcctatagaa aggaaaggat 901 tttatctggc ttttcaagac attggggcgt gcattgccct ggtttcagtc cgtgttttct 961 acaagaaatg ccccttcact gttcgtaact tggccatgtt tcctgatacc attccaaggg 1021 ttgattcctc ctctttggtt gaagtacggg gttcttgtgt gaagagtgct gaagagcgtg 1081 acactcctaa actgtattgt ggagctgatg gagattggct ggttcctctt ggaaggtgca 1141 tctgcagtac aggatatgaa gaaattgagg gttcttgcca tgcttgcaga ccaggattct 1201 ataaagcttt tgctgggaac acaaaatgtt ctaaatgtcc tccacacagt ttaacataca 1261 tggaagcaac ttctgtctgt cagtgtgaaa agggttattt ccgagctgaa aaagacccac 1321 cttctatggc atgtaccagg ccaccttcag ctcctaggaa tgtggttttt aacatcaatg 1381 aaacagccct tattttggaa tggagcccac caagtgacac aggagggaga aaagatctca 1441 catacagtgt aatctgtaag aaatgtggct tagacaccag ccagtgtgag gactgtggtg 1501 gaggactccg cttcatccca agacatacag gcctgatcaa caattccgtg atagtacttg 1561 actttgtgtc tcacgtgaat tacacctttg aaatagaagc aatgaatgga gtttctgagt 1621 tgagtttttc tcccaagcca ttcacagcta ttacagtgac cacggatcaa gatgcacctt 1681 ccctgatagg tgtggtaagg aaggactggg catcccaaaa tagcattgcc ctatcatggc 1741 aagcacctgc tttttccaat ggagccattc tggactacga gatcaagtac tatgagaaag 1801 aacatgagca gctgacctac tcttccacaa ggtccaaagc ccccagtgtc atcatcacag 1861 gtcttaagcc agccaccaaa tatgtatttc acatccgagt gagaactgcg acaggataca 1921 gtggctacag tcagaaattt gaatttgaaa caggagatga aacttctgac atggcagcag 1981 aacaaggaca gattctcgtg atagccaccg ccgctgttgg cggattcact ctcctcgtca 2041 tcctcacttt attcttcttg atcactggga gatgtcagtg gtacataaaa gccaagatga 2101 agtcagaaga gaagagaaga aaccacttac agaatgggca tttgcgcttc ccgggaatta 2161 aaacttacat tgatccagat acatatgaag acccatccct agcagtccat gaatttgcaa 2221 aggagattga tccctcaaga attcgtattg agagagtcat tggggcaggt gaatttggag 2281 aagtctgtag tgggcgtttg aagacaccag ggaaaagaga gatcccagtt gccattaaaa 2341 ctttgaaagg tggccacatg gatcggcaaa gaagagattt tctaagagaa gctagtatca 2401 tgggccagtt tgaccatcca aacatcattc gcctagaagg ggttgtcacc aaaagatcct 2461 tcccggccat tggggtggag gcgttttgcc ccagcttcct gagggcaggg tttttaaata 2521 gcatccaggc cccgcatcca gtgccagggg gaggatcttt gccccccagg attcctgctg 2581 gcagaccagt aatgattgtg gtggaatata tggagaatgg atccctagac tcctttttgc 2641 ggaagcatga tggccacttc acagtcatcc agttggtcgg aatgctccga ggcattgcat 2701 caggcatgaa gtatctttct gatatgggtt atgttcatcg agacctagcg gctcggaata 2761 tactggtcaa tagcaactta gtatgcaaag tttctgattt tggtctctcc agagtgctgg 2821 aagatgatcc agaagctgct tatacaacaa ctggtggaaa aatccccata aggtggacag 2881 ccccagaagc catcgcctac agaaaattct cctcagcaag cgatgcatgg agctatggca 2941 ttgtcatgtg ggaggtcatg tcctatggag agagacctta ttgggaaatg tctaaccaag 3001 atgtcattct gtccattgaa gaagggtaca gacttccagc tcccatgggc tgtccagcat 3061 ctctacacca gctgatgctc cactgctggc agaaggagag aaatcacaga ccaaaattta 3121 ctgacattgt cagcttcctt gacaaactga tccgaaatcc cagtgccctt cacaccctgg 3181 tggaggacat ccttgtaatg ccagagtccc ctggtgaagt tccggaatat cctttgtttg 3241 tcacagttgg tgactggcta gattctataa agatggggca atacaagaat aacttcgtgg 3301 cagcagggtt tacaacattt gacctgattt caagaatgag cattgatgac attagaagaa 3361 ttggagtcat acttattgga caccagagac gaatagtcag cagcatacag actttacgtt 3421 tacacatgat gcacatacag gagaagggat ttcatgtatt gccaactttc ttgtacaaag 3481 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3541 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta