Construct: ORF ccsbBroadEn_02416

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013520.1_s300c1, BRDN0000386340
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB4B (10383)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 100% 100%
2 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 88.6% 90.7% (many diffs)
3 human 260334 TUBB8B tubulin beta 8B NM_001358689.1 88.2% 88.9% (many diffs)
4 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 87.2% 98.4% (many diffs)
5 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 87.2% 98.4% (many diffs)
6 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 87.2% 96.4% (many diffs)
7 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 86.4% 96.8% (many diffs)
8 human 203068 TUBB tubulin beta class I NM_178014.4 84.4% 97.3% (many diffs)
9 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 84% 91.7% (many diffs)
10 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 83.2% 90.5% (many diffs)
11 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 83.2% 90.5% (many diffs)
12 human 203068 TUBB tubulin beta class I NM_001293212.2 79.6% 90.3% (many diffs)
13 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 79.2% 89.3% (many diffs)
14 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 78.2% 88.3% (many diffs)
15 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 77.4% 83.1% (many diffs)
16 human 203068 TUBB tubulin beta class I NM_001293214.2 75.5% 87.8% (many diffs)
17 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 74.4% 76.6% (many diffs)
18 human 260334 TUBB8B tubulin beta 8B XM_024451145.1 74% 75.5% (many diffs)
19 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 73.4% 82.4% (many diffs)
20 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 73.4% 82.4% (many diffs)
21 human 260334 TUBB8B tubulin beta 8B XM_024451143.1 71.8% 53.8% (many diffs)
22 human 260334 TUBB8B tubulin beta 8B XM_024451144.1 71.8% 53.8% (many diffs)
23 human 260334 TUBB8B tubulin beta 8B XM_024451146.1 71.6% 72.8% (many diffs)
24 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 71.4% 77.7% (many diffs)
25 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 71.2% 71.2% (many diffs)
26 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 70.7% 76.9% (many diffs)
27 human 203068 TUBB tubulin beta class I NM_001293215.2 70.5% 82% (many diffs)
28 human 203068 TUBB tubulin beta class I NM_001293216.2 70.5% 82% (many diffs)
29 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 70.4% 76.7% (many diffs)
30 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 66.3% 68.3% (many diffs)
31 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 66.3% 68.3% (many diffs)
32 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 65.4% 67.4% (many diffs)
33 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 57.4% 61.6% (many diffs)
34 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 57.4% 61.6% (many diffs)
35 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519460.2 57% 56.6% (many diffs)
36 human 260334 TUBB8B tubulin beta 8B XM_024451148.1 56.8% 55.9% (many diffs)
37 human 203068 TUBB tubulin beta class I NM_001293213.2 46.5% 52.1% (many diffs)
38 human 643224 TUBBP5 tubulin beta pseudogene 5 NR_027156.1 43.7% (many diffs)
39 human 203068 TUBB tubulin beta class I NR_120608.2 24.9% (many diffs)
40 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 90.1% 100% (many diffs)
41 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 85.8% 96.4% (many diffs)
42 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 85.4% 96.8% (many diffs)
43 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 84.1% 97.3% (many diffs)
44 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 83.9% 98.4% (many diffs)
45 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 83.9% 91.5% (many diffs)
46 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 82.2% 90.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1401
ORF length:
1335
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG GGAAATCGTG CACTTGCAGG CCGGGCAGTG CGGCAACCAA ATCGGCGCCA
121AGTTTTGGGA GGTGATCAGC GATGAGCACG GCATCGACCC CACGGGCACC TACCACGGGG
181ACAGCGACCT GCAGCTGGAA CGCATCAACG TGTACTACAA TGAGGCCACC GGCGGCAAGT
241ACGTGCCCCG CGCCGTGCTC GTGGATCTGG AGCCCGGCAC CATGGACTCC GTGCGCTCGG
301GGCCCTTCGG GCAGATCTTC CGGCCGGACA ACTTCGTTTT CGGTCAGAGT GGTGCTGGGA
361ACAACTGGGC CAAGGGGCAC TACACAGAAG GCGCGGAGCT GGTGGACTCG GTGCTGGATG
421TTGTGAGAAA GGAGGCTGAG AGCTGTGACT GCCTGCAGGG TTTCCAGCTG ACCCACTCCC
481TGGGTGGGGG GACTGGGTCT GGGATGGGTA CCCTCCTCAT CAGCAAGATC CGGGAGGAGT
541ACCCAGACAG GATCATGAAC ACGTTTAGTG TGGTGCCTTC GCCCAAAGTG TCAGACACAG
601TGGTGGAGCC CTACAACGCC ACCCTCTCAG TCCACCAGCT CGTAGAAAAC ACAGACGAGA
661CCTACTGCAT TGATAACGAA GCTCTCTACG ACATTTGCTT CAGAACCCTA AAGCTGACCA
721CGCCCACCTA TGGTGACCTG AACCACCTGG TGTCTGCTAC CATGAGTGGG GTCACCACCT
781GCCTGCGCTT CCCAGGCCAG CTCAATGCTG ACCTGCGGAA GCTGGCTGTG AACATGGTCC
841CGTTTCCCCG GCTGCACTTC TTCATGCCCG GCTTTGCCCC ACTGACCAGC CGGGGCAGCC
901AGCAGTACCG GGCGCTGACC GTGCCCGAGC TCACCCAGCA GATGTTTGAT GCCAAGAACA
961TGATGGCTGC CTGCGACCCC CGCCATGGCC GCTACCTGAC GGTTGCCGCC GTGTTCAGGG
1021GCCGCATGTC CATGAAGGAG GTGGATGAGC AAATGCTTAA TGTCCAAAAC AAAAACAGCA
1081GCTATTTTGT TGAGTGGATC CCCAACAATG TGAAAACGGC TGTCTGTGAC ATCCCACCTC
1141GGGGGCTAAA AATGTCCGCC ACCTTCATTG GCAACAGCAC GGCCATCCAG GAGCTGTTCA
1201AGCGCATCTC CGAGCAGTTC ACGGCCATGT TCCGGCGCAA GGCCTTCCTG CACTGGTACA
1261CGGGCGAGGG CATGGACGAG ATGGAGTTCA CCGAGGCCGA GAGCAACATG AATGACCTGG
1321TGTCCGAGTA CCAGCAGTAC CAGGATGCCA CAGCCGAGGA GGAGGGCGAG TTCGAGGAGG
1381AGGCTGAGGA GGAGGTGGCC TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
1441attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)