Construct: ORF ccsbBroadEn_06931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005955.1_s300c1, BRDN0000390496
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEMA3F (6405)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6405 | SEMA3F | semaphorin 3F | NM_004186.5 | 99.9% | 99.8% | 1507T>A |
| 2 | human | 6405 | SEMA3F | semaphorin 3F | XM_005265381.4 | 99.9% | 99.8% | 1507T>A |
| 3 | human | 6405 | SEMA3F | semaphorin 3F | XM_011533998.2 | 99.9% | 99.8% | 1507T>A |
| 4 | human | 6405 | SEMA3F | semaphorin 3F | NM_001318800.1 | 96% | 95.9% | 452_453ins93;1414T>A |
| 5 | human | 6405 | SEMA3F | semaphorin 3F | XM_005265382.4 | 96% | 95.9% | 452_453ins93;1414T>A |
| 6 | human | 6405 | SEMA3F | semaphorin 3F | XR_940487.2 | 89.2% | (many diffs) | |
| 7 | human | 6405 | SEMA3F | semaphorin 3F | NM_001318798.2 | 87.3% | 87.2% | 0_1ins204;248_249ins93;1210T>A |
| 8 | human | 6405 | SEMA3F | semaphorin 3F | XM_006713289.3 | 74.2% | 72.7% | (many diffs) |
| 9 | human | 6405 | SEMA3F | semaphorin 3F | XM_011534000.2 | 62.4% | 63% | 1454_1493del;1536_1537ins859 |
| 10 | human | 6405 | SEMA3F | semaphorin 3F | XM_006713290.3 | 61.2% | 60.6% | (many diffs) |
| 11 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | NM_001311151.1 | 89% | 95.9% | (many diffs) |
| 12 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XM_017313228.1 | 89% | 95.9% | (many diffs) |
| 13 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | NM_011349.3 | 85.6% | 92.6% | (many diffs) |
| 14 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XM_006511676.3 | 85.6% | 92.6% | (many diffs) |
| 15 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XM_017313226.1 | 78.6% | 76.6% | (many diffs) |
| 16 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XM_017313227.1 | 75.4% | 73.5% | (many diffs) |
| 17 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XR_379758.3 | 53.2% | (many diffs) | |
| 18 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XR_871078.2 | 53.2% | (many diffs) | |
| 19 | mouse | 20350 | Sema3f | sema domain, immunoglobulin... | XR_379759.3 | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2421
- ORF length:
- 2355
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCT TGTCGCCGGT CTTCTTCTCT GGGCTTCCCT ACTGACCGGG GCCTGGCCAT 121 CCTTCCCCAC CCAGGACCAC CTCCCGGCCA CGCCCCGGGT CCGGCTCTCA TTCAAAGAGC 181 TGAAGGCCAC AGGCACCGCC CACTTCTTCA ACTTCCTGCT CAACACAACC GACTACCGAA 241 TCTTGCTCAA GGACGAGGAC CACGACCGCA TGTACGTGGG CAGCAAGGAC TACGTGCTGT 301 CCCTGGACCT GCACGACATC AACCGCGAGC CCCTCATTAT ACACTGGGCA GCCTCCCCAC 361 AGCGCATCGA GGAATGCGTG CTCTCAGGCA AGGATGTCAA CGGCGAGTGT GGGAACTTCG 421 TCAGGCTCAT CCAGCCCTGG AACCGAACAC ACCTGTATGT GTGCGGGACA GGTGCCTACA 481 ACCCCATGTG CACCTATGTG AACCGCGGAC GCCGCGCCCA GGCCACACCA TGGACCCAGA 541 CTCAGGCGGT CAGAGGCCGC GGCAGCAGAG CCACGGATGG TGCCCTCCGC CCGATGCCCA 601 CAGCCCCACG CCAGGATTAC ATCTTCTACC TGGAGCCTGA GCGACTCGAG TCAGGGAAGG 661 GCAAGTGTCC GTACGATCCC AAGCTGGACA CAGCATCGGC CCTCATCAAT GAGGAGCTCT 721 ATGCTGGTGT GTACATCGAT TTTATGGGCA CTGATGCAGC CATCTTCCGC ACACTTGGAA 781 AGCAGACAGC CATGCGCACG GATCAGTACA ACTCCCGGTG GCTGAACGAC CCGTCGTTCA 841 TCCATGCTGA GCTCATTCCT GACAGTGCGG AGCGCAATGA TGATAAGCTT TACTTCTTCT 901 TCCGTGAGCG GTCGGCAGAG GCGCCGCAGA GCCCCGCGGT GTACGCCCGC ATCGGGCGCA 961 TTTGCCTGAA CGATGACGGT GGTCACTGTT GCCTGGTCAA CAAGTGGAGC ACATTCCTGA 1021 AGGCGCGGCT CGTCTGCTCT GTCCCGGGCG AGGATGGCAT TGAGACTCAC TTTGATGAGC 1081 TCCAGGACGT GTTTGTCCAG CAGACCCAGG ACGTGAGGAA CCCTGTCATT TACGCTGTCT 1141 TTACCTCCTC TGGCTCCGTG TTCCGAGGCT CTGCCGTGTG TGTCTACTCC ATGGCTGATA 1201 TTCGCATGGT CTTCAACGGG CCCTTTGCCC ACAAAGAGGG GCCCAACTAC CAGTGGATGC 1261 CCTTCTCAGG GAAGATGCCC TACCCACGGC CGGGCACGTG CCCTGGTGGA ACCTTCACGC 1321 CATCTATGAA GTCCACCAAG GATTATCCTG ATGAGGTGAT CAACTTCATG CGCAGCCACC 1381 CACTCATGTA CCAGGCCGTG TACCCTCTGC AGCGGCGGCC CCTGGTAGTC CGCACAGGTG 1441 CTCCCTACCG CCTTACCACT ATTGCCGTGG ACCAGGTGGA TGCAGCCGAC GGGCGCTATG 1501 AGGTGCTTTT CCTGGGCACA GACCGCGGGA CAGTGCAGAA GGTCATTGTG CTGCCCAAGG 1561 ATGACCAGGA GATGGAGGAG CTCATGCTGG AGGAGGTGGA GGTCTTCAAG GATCCAGCAC 1621 CCGTCAAGAC CATGACCATC TCTTCTAAGA GGCAACAACT CTACGTGGCG TCAGCCGTGG 1681 GTGTCACACA CCTGAGCCTG CACCGCTGCC AGGCGTATGG GGCTGCCTGT GCTGACTGCT 1741 GCCTTGCCCG GGACCCTTAC TGTGCCTGGG ATGGCCAGGC CTGCTCCCGC TATACAGCAT 1801 CCTCCAAGAG GCGGAGCCGC CGGCAGGACG TCCGGCACGG AAACCCCATC AGGCAGTGCC 1861 GTGGGTTCAA CTCCAATGCC AACAAGAATG CCGTGGAGTC TGTGCAGTAT GGCGTGGCCG 1921 GCAGCGCAGC CTTCCTTGAG TGCCAGCCCC GCTCGCCCCA AGCCACTGTT AAGTGGCTGT 1981 TCCAGCGAGA TCCTGGTGAC CGGCGCCGAG AGATTCGTGC AGAGGACCGC TTCCTGCGCA 2041 CAGAGCAGGG CTTGTTGCTC CGTGCACTGC AGCTCAGCGA TCGTGGCCTC TACTCCTGCA 2101 CAGCCACTGA GAACAACTTT AAGCACGTCG TCACACGAGT GCAGCTGCAT GTACTGGGCC 2161 GGGACGCCGT CCATGCTGCC CTCTTCCCAC CACTGTCCAT GAGCGCCCCG CCACCCCCAG 2221 GCGCAGGCCC CCCAACGCCT CCTTACCAGG AGTTAGCCCA GCTGCTGGCC CAGCCAGAAG 2281 TGGGCCTCAT CCACCAGTAC TGCCAGGGTT ACTGGCGCCA TGTGCCCCCC AGCCCCAGGG 2341 AGGCTCCAGG GGCACCCCGG TCTCCTGAGC CCCAGGACCA GAAAAAGCCC CGGAACCGCC 2401 GGCACCACCC TCCGGACACA TGCCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc 2461 attgcttatc aatttgttgc aacgaac