Construct: ORF ccsbBroadEn_08635
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001484.1_s300c1, BRDN0000394703
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF253 (56242)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_021047.3 | 99.8% | 100% | 1005A>T;1017C>T |
| 2 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_001331133.1 | 87% | 87.1% | 0_1ins192;813A>T;825C>T |
| 3 | human | 56242 | ZNF253 | zinc finger protein 253 | NM_001331134.1 | 84.6% | 84.7% | 0_1ins228;777A>T;789C>T |
| 4 | human | 199777 | ZNF626 | zinc finger protein 626 | NM_001076675.3 | 82.2% | 71% | (many diffs) |
| 5 | human | 100129842 | ZNF737 | zinc finger protein 737 | NM_001159293.2 | 80.5% | 69.7% | (many diffs) |
| 6 | human | 440515 | ZNF506 | zinc finger protein 506 | NM_001099269.3 | 79.2% | 69.6% | (many diffs) |
| 7 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_006722597.4 | 78.5% | 68% | (many diffs) |
| 8 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_011527599.3 | 78.5% | 68% | (many diffs) |
| 9 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_005259697.4 | 78.4% | 67.8% | (many diffs) |
| 10 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_011527598.3 | 78.4% | 67.8% | (many diffs) |
| 11 | human | 90649 | ZNF486 | zinc finger protein 486 | NM_052852.4 | 77% | 65.6% | (many diffs) |
| 12 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_017026116.1 | 74.8% | 63.9% | (many diffs) |
| 13 | human | 7617 | ZNF66 | zinc finger protein 66 | NM_001355197.2 | 74.2% | 64.2% | (many diffs) |
| 14 | human | 440515 | ZNF506 | zinc finger protein 506 | NM_001145404.2 | 73.3% | 63.8% | (many diffs) |
| 15 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_003429.5 | 72% | 60.8% | (many diffs) |
| 16 | human | 81931 | ZNF93 | zinc finger protein 93 | NM_031218.4 | 69.3% | 58.7% | (many diffs) |
| 17 | human | 7643 | ZNF90 | zinc finger protein 90 | NM_007138.2 | 69.1% | 58% | (many diffs) |
| 18 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256171.1 | 68.6% | 57.7% | (many diffs) |
| 19 | human | 440519 | ZNF724 | zinc finger protein 724 | NM_001355404.2 | 68.2% | 58.3% | (many diffs) |
| 20 | human | 100129842 | ZNF737 | zinc finger protein 737 | XM_017026117.2 | 67.4% | 56.7% | (many diffs) |
| 21 | human | 7639 | ZNF85 | zinc finger protein 85 | XM_011528263.2 | 67% | 56.5% | (many diffs) |
| 22 | human | 148213 | ZNF681 | zinc finger protein 681 | NM_138286.3 | 65.7% | 54.9% | (many diffs) |
| 23 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256173.1 | 62.6% | 51.7% | (many diffs) |
| 24 | human | 100129842 | ZNF737 | zinc finger protein 737 | XR_001753570.2 | 60.3% | (many diffs) | |
| 25 | human | 7608 | ZNF56 | zinc finger protein 56 | NM_001355194.2 | 41.6% | 32.8% | (many diffs) |
| 26 | human | 105372319 | LOC105372319 | zinc finger protein 430-like | XM_017027571.1 | 32.3% | 21.6% | (many diffs) |
| 27 | human | 7643 | ZNF90 | zinc finger protein 90 | XR_001753753.2 | 30.3% | (many diffs) | |
| 28 | human | 440519 | ZNF724 | zinc finger protein 724 | XM_024451521.1 | 13.6% | 12.6% | (many diffs) |
| 29 | human | 51427 | ZNF107 | zinc finger protein 107 | XM_024446791.1 | 13.2% | 11.6% | (many diffs) |
| 30 | human | 7697 | ZNF138 | zinc finger protein 138 | NM_001271637.2 | 10.9% | 8.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1566
- ORF length:
- 1497
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGGACCATTG CAATTTAGAG ATGTGGCCAT AGAATTCTCT CTGGAGGAGT 121 GGCATTGCCT GGACACTGCA CAGCGGAATT TATATAGGGA TGTGATGTTA GAGAACTACA 181 GAAACTTGGT CTTCCTTGGT ATTGTTGTCT CTAAGCCAGA CCTGGTTACC TGTCTGGAGC 241 AAGGAAAAAA ACCTTTAACT ATGGAAAGAC ATGAGATGAT TGCCAAACCC CCAGTTATGA 301 GTTCTCATTT TGCCCAAGAC CTTTGGCCAG AGAACATACA AAATTCTTTC CAAATAGGGA 361 TGCTGAGAAG ATATGAAGAA TGCAGACATG ACAATTTACA GTTAAAAAAA GGCTGTAAAA 421 GCGTGGGTGA GCATAAGGTG CACAAAGGAG GTTATAATGG ACTTAACCAA TGTTTGACAA 481 CTACCCAGAA AGAAATATTT CAATGTGATA AATATGGAAA AGTCTTTCAT AAGTTTTCAA 541 ATTCAAACAC ATATAAGACA AGACATACTG GAATAAATCT TTTCAAATGT ATAATATGTG 601 GCAAAGCTTT TAAACGGTCC TCAACCCTTA CTACACATAA GAAAATTCAT ACTGGAGAGA 661 AACCTTACAG ATGTGAAGAA TGTGGCAAAG CTTTTAACCA ATCTGCAAAC CTTACTACAC 721 ATAAGAGAAT TCATACCGGA GAGAAACCCT ACAGATGTGA AGAATGTGGC AAAGCCTTTA 781 AGCAGTCCTC AAACCTTACT ACACATAAGA AAATTCATAC TGGAGAGAAA CCCTACAAAT 841 GTGAAGAATG TGGCAAAGCC TTCAACCGAT CCACAGACCT TACTACACAT AAGATAGTTC 901 ATACTGGAGA GAAACCCTAC AAATGTGAAG AATGTGGCAA AGCCTTTAAG CACCCCTCAC 961 ACGTTACCAC ACATAAGAAA ATTCATACTA GAGGGAAACC CTACAACTGT GAAGAATGTG 1021 GCAAATCCTT TAAGCACTGC TCTAACCTTA CTATACATAA GAGAATTCAT ACTGGAGAGA 1081 AACCTTACAA ATGTGAAGAA TGTGGCAAAG CCTTTCACCT ATCCTCACAC CTTACTACAC 1141 ATAAGATACT TCATACTGGA GAGAAACCCT ACAGATGTAG AGAATGTGGC AAAGCTTTTA 1201 ACCATTCCAC AACCCTTTTT TCACATGAGA AAATTCATAC TGGAGAGAAA CCCTACAAAT 1261 GTGATGAATG TGGCAAAACC TTTACCTGGC CCTCAATCCT CTCCAAACAT AAAAGAACTC 1321 ATACTGGAGA GAAACCCTAC AAATGTGAAG AATGTGGCAA ATCCTTTACT GCATCCTCAA 1381 CTCTAACTAC ACATAAGAGA ATTCATACTG GAGAGAAACC TTACAAATGT GAAGAATGTG 1441 GCAAAGCTTT TAACTGGTCC TCAGACCTTA ATAAACATAA GAAAATTCAT ATTGAACGAA 1501 AACCCTACAT AGTGAAGAAT GTGACAGATC TTTTAAATGT TCCTCCACTT TTAATTAGCA 1561 TAAGATTGCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 1621 gttgcaacga ac